DNA isolation Protocol

mobiolaboratoriesServeurs

3 févr. 2015 (il y a 2 années et 9 mois)

202 vue(s)

MO BIO Laboratories providing innovative molecular biology research tools for DNA, RNA, protein isolation, extraction and purification. Call (800) 606-6246.

DNA isolation Protocol
MO BIO Services
MO
BIO
Laboratories
offers
testing
that
keeps
your
product
lines
competitive
and
in
demand
.
Our
services
specialists
can
also
work
with
you
to
develop
a
custom
testing
schedule
and/or
custom
tests
that
fit
the
specific
needs
of
your
products
.
MO
BIO
Laboratories
is
an
ISO
9001
:
2008
certified
company
.
CERTIFICATIONS

Arrow
DNA
-
Free
Certification

Arrow
RNase
-
Free
Certification

Arrow
DNase
-
Free
Certification

Arrow
ATP
-
Free
Certification

Arrow
Protease
-
Free
Certification

Arrow
Endotoxin
-
Free
Certification

Arrow
Endonuclease
-
Free
Certification
DNA
-
Free Certification
This
test
certifies
any
of
a
wide
variety
of
products
to
be
free
of
certain
types
of
DNA
and
common
contaminants
and
inhibitors
that
interfere
with
the
PCR
process
.
Features

Certify
your
lab
supplies
and
products
as
DNA
-
free
.
Certficate
of
Analysis
is
provided
for
each
lot
number
tested

Two
methods
available
--
choose
your
prefered
level
of
sensitivity

Custom
extraction
protocols
can
developed
for
each
product
Test
samples
are
extracted
and
a
portion
of
extract
is
added
to
a
multiplex
PCR
reaction
containing
primers
specific
for
human
and
mouse
genomic
DNA
.
These
tubes
receive
no
template
DNA
so
any
amplification
in
these
tubes
will
indicate
the
presence
of
contaminating
DNA
.
A
negative
control
reaction
is
done
with
DNA
-
Free
water
as
a
reference
.
RNase
-
Free Certification
A
wide
variety
of
products
are
certified
to
be
free
of
RNase
contamination
and
ready
for
direct
use
in
all
RNA
work
using
this
test
.
Features

Certify
your
products
and
lab
supplies
are
free
of
detecable
levels
of
RNase
contamination

Certificate
of
Analysis
is
included
for
each
product
lot
number
tested

Test
Sensitivity
:
10
-
9
Kunitz
Units/
µl
All
testing
apparatus
are
treated
with
Diethyl
Pyrocarbonate
(DEPC)
to
inhibit
possible
RNase
contamination
of
the
experiment
from
outside
sources
.
Test
samples
of
the
product
are
extracted
and
a
portion
of
extract
is
incubated
with
an
RNA
standard
(
6
kb
RNA)
in
a
buffered
solution
with
both
sodium
and
magnesium
ions
DNase
-
Free Certification
This
test
certifies
products
to
be
free
of
DNase
contamination
and
ready
for
use
in
any
of
the
many
experiments
and
applications
of
DNA
.
Features

Certify
your
products
and
lab
supplies
are
free
of
detecable
levels
of
DNase
contamination

Certificate
of
Analysis
is
included
for
each
product
lot
number
tested

Test
Sensitivity
:
10
-
7
Kunitz
Units/
All
testing
apparatus
are
treated
with
Diethyl
pyrocarbonate
(DEPC)
to
inhibit
possible
DNase
contamination
of
the
experiment
from
outside
sources
.
Test
samples
of
the
product
are
extracted
and
a
portion
of
extract
is
incubated
with
DNA
standard
(
1
kb
DNA
ladder)
in
a
buffered
solution
.
ATP
-
Free Certification
This
test
is
used
to
certify
a
variety
of
products
to
be
free
of
contaminating
ATP
molecules
which
can
interfere
with
several
types
of
enzymatic
reactions
and
also
further
indicate
the
presence
of
any
biological
activity
.
Features

Highly accurate
--
luciferase
-
based assay allows for the most accurate detection of
ATP levels

More sensitive than other methods
--
test sensitivity of
10
-
13
mg/µl

Certficate of Analysis
--
provided to document that your products and lab supplies
are ATP
-
Free.
A luciferase
-
based assay is used to detect the level of ATP in a sample of product.
Luciferase is the enzyme found in fireflies that causes them to emit light. The
luciferase enzyme catalyzes an ATP dependent oxidation of the luciferin substrate
(shown in the reaction below).
Endotoxin
-
Free Certification
This
certification
follows
USP
guidelines
and
FDA
regulations
to
certify
any
of
a
variety
of
products
to
be
free
of
contaminating
endotoxins
or
to
quantify
endotoxin
levels
.
Features

Certify that your lab supplies and products are free of endotoxin contamination

Test Sensitivity of
0.06 EU/ml

Certificate of Analysis and Data & Results sheet provided to document your products
are Endotoxin
-
Free
MO BIO Laboratories, Inc. and all its testing technicians have been qualified
according to FDA regulations to utilize the LAL (Limulus Amebocyte Lysate) Gel Clot
method to test raw materials or end products for the presence of endotoxins. This
method uses components found in the blood of the Blue Horseshoe Crab, Limulus
polyphemus, which forms a gel
-
like clot when incubated in the presence of
endotoxins.
About
MO
BIO
Laboratories,
Inc
.
MO
BIO
Laboratories,
a
privately
held
company
located
in
the
San
Diego
biotech
corridor
(Carlsbad,
CA
USA),
was
founded
in
1993
.
Initially
a
small
business
start
-
up,
MO
BIO
has
grown
into
a
multi
-
million
dollar
operation
with
international
production,
research,
and
distribution
.
Today,
MO
BIO's
growth
and
customer
loyalty
is
challenging
some
of
the
world's
leading
molecular
biology
tool
companies
with
innovative
technologies
and
a
commitment
to
value
.
MO
BIO
is
the
World
Leader
in
Soil
DNA
&
RNA
Isolation
®
.
One
of
the
fastest
growing
companies
in
Life
Science,
the
product
innovation,
company
philosophy,
and
customer
service
focus
continues
to
draw
customers
and
outstanding
industry
talent
to
MO
BIO
.
The
founders
and
employees
of
MO
BIO
are
dedicated
to
the
preservation
of
the
environment
and
to
bettering
the
quality
of
the
Earth
through
science
.
Programs
New Lab Program
For
nearly
20
years,
MO
BIO
Laboratories
inc
.
has
been
committed
to
helping
further
scientific
research,
through
providing
cutting
edge
DNA
and
RNA
isolation
kits
and
technology
.
To
enhance
this
commitment
to
the
scientific
community,
MO
BIO
has
established
the
New
Lab
Start
Up
Program
to
support
the
very
research
that
made
MO
BIO
what
is
it
today
.
Eligible
participants
will
receive
a
choice
of
FREE
MO
BIO
New
Lab
Start
-
Up
Package
valued
at
over
$
462
after
a
purchase
of
over
$
1500
.
Or
MO
BIO
New
Lab
Start
-
Up
Package
at
50
%
discount
.

UltraClean®
PCR
Clean
-
Up
Kit
Cat
#
12500
-
50

RTS
DNase
Kit
Cat
#
15200
-
50

UltraClean®
GelSpin®
DNA
Extraction
Kit
Cat
#
12400
-
50

PCR
Grade
Water,
10
x
1
ml
Cat
#
17000
-
10
Vortex
Adapter,
Genie,
holds
24
(
1
.
5
-
2
.
0
ml)
tubes
Cat
#
13000
-
V
1
-
24

Up
to
four
free
samples
of
your
choice!
MO
BIO Laboratories, Inc
.
2746 Loker Avenue
West
Carlsbad, CA 92010
For
more information please
visit
http
://
www.mobio.com
Toll
-
free in US: 800
-
606
-
6246