Biotechnology Fall Exam Review 2011

Fall 20
1
1

Biotech 1 Review Guide

1

Biotechnology Fall Exam Review

201
1


Biotechnology Introduction

1.

Define Biotechnology

2.

How has biotechnology changed through the decades?

3.

What are the domains or key areas of biotechnology? (text)

4.

List 10 Groundbreaking discoveries that are considered biotec
hnology.


Legal
-
Scientific Notebook

5.

Why is it important to keep a scientific notebook in a laboratory?

6.

How is a scientific notebook set
-
up and kept on a daily basis?


Safety

7.

What are MSDS data sheets and what information to they contain?

8.

What are some safe
ty “do’s and don’ts” in a laboratory?

9.

What are the 5 colors of the Chemical Storage System used at MHS?

10.

Define PPE, MSDS, GLP
, SLOP


Scientific Methodology

11.

Describe the characteristics of a good hypothesis.

12.

What are the components of a valid experiment?

13.

Wh
y are well written, concise protocols important in science?

14.

What do

the following components of the analysis mean: “REE, PE, PA”
?

and give an
example from one of the labs performed this semester.


Organic Chemistry

15.

In the cheese
-
making lab, what was the in
dependent variable?

dependent variable?


16.

What are chymosin and rennin?


Measurement in the Laboratory

17.

Describe how you would convert a number to scientific notation.

18.

How do you convert larger metric units to smaller units? Smaller to larger?

19.

What are the

metric prefixes for

10
0
,

10
-
3
, 10
-
6
, 10
-
9
,
10
-
12
,
10
-
15


20.

What are the rules for using a micropipet?

Draw pictures of this process.

21.

How do you read the following micropipets?
P
-
10, P
-
20, P
-
200, P
-
1000
. Draw diagram
of a sample reading for each and label t
he units for each number.


P
-
10

1

5

4




List the numbers and tell the volume you have selected & list the color of tips needed for each micropipette

P
-
10






P
-
20

1

5

4


P
-
200

1

5

4


P
-
1000

0

1

5


P
-
20





P
-
200





P
-
1000





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Biotech 1 Review Guide

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22.

List the appropriate volumes/masses tha
t each of the following measure:

a.

p
-
20,

b.

p
-
200,

c.

p
-
1000,

d.

10 ml pipet,

e.

1 ml pipet,

f.

graduated cylinder,

g.

analytical balance
,

h.

table top balance



23.

How do you dispose of practice tips vs. autoclaved (sterile) tips?

24.

What are 4 things a technician can do to minimize/eliminate sources of error when
using the micropipettes?

25.

What are 4 things a technician can do to minimize/eliminate sources of error when
using the serological pipettes?

26.

What is the major rule when loading a centrifuge?

Draw a
diagram

too!

27.

What is the difference between an analytical ba
lance and table top balances used
at
your bench
?

28.

Explain in detail how to measure the following masses:

a.

2.1 grams

b.

0.1 milligrams

29.

What are 4 things a technician can do to minimize/eliminate sources of error when
using the balances?


Microbiology

30.

Define Asep
tic Technique.

31.

Why does Aseptic Technique matter?

32.

Define Model Organism.

33.

Why is
E. coli

a good model organism for microbiology?

34.

Why 37°C for
E. coli
?

35.

Why do I keep italicizing
E. coli
?

36.

Why are plates stored upside down?

37.

Define media (bacterial….not the TV)
.

38.

Don’t you want a snack right now after all these questions?

39.

What is the difference in composition of agar and broth?

40.

What is a Gram stain? (Be sure to give steps and results after each step too!)

41.

Why do some cells stain pink and others purple during Gram

staining?

42.

How does a microscope work?


Spectrophotometry

43.

What is a s
pectrophotometer
?

Be able to label and identify parts and function of each.

44.

Describe the steps to using a spectrophotometer in biotech class.

45.

What is the Beer
-
Lambert Law and how does it
apply to biotechnology?

Diagram a
picture to demonstrate this Law.

46.

What are the two ways of measuring molecules in solution?

47.

Define Indicator

48.

Give an example of 3 indicators, what they indicate for, and what the reaction is for a
positive result.

49.

What does

a spec measure?

50.

What are some applications for the use of a spec in the “real world”

51.

Define standard curve
.

Also, given a standard curve
–

be able to determine the
concentration of an unknown solution based on its absorbance.

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Biotech 1 Review Guide

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52.

What is the purpose of an ab
sorbance spectrum?

You made one with the four colors
and with CuSO
4
x5H
2
O…

53.

What are 4 things a technician can do to minimize/eliminate sources of error when
using the spec?


54.

What is the advantage of a microplate reader vs. the Spec20D?


Solutions/Dilutions

55.

List the formulas for each of the following types of solutions:

a.

mass/volume
:

b.

% mass/volume
:

c.

Dilutions

56.

What are the components of a 5 ml of a 50 mg/ml copper sulfate pentahydrate
solution?

57.

What is the correct formula for determining the mass of NaCl needed
for 5 mL of a
0.1% NaCl solution

58.

Given the following solution,
3 mg NaCl in a total of 100 ml of water

determine
the
:


a.

mass/volume concentration and

b.

% mass/volu
me concentration. Show all your calculations.



59.

How would you make 60ml of a 1X NaCl solution f
rom a 20X NaCl solution?

60.

Su
zie made a solution by putting
0.25g of copper sulfate pen
tahydrate in
a graduated
cylinder and water to make a total volume of 50 mL.


a.

What is the mass/volume concentration of this solution?

b.

What is the %mass/volume concentrati
on of this solution?


61.

Define qualitative and quantitative data AND give
s
pecific

examples of each.

62.

W
hat is the purpose of the
chemical
fume hood?

63.

W
here do you dispose of your lab waste? Give examples of 4 types of waste and how
they are disposed?


Bonus
Questions:

What is biuret solution, and when would it be used in the lab? Give positive and negative
reactions for when it is used.


Explain how a micropipette draws up and dispenses samples (e.g. explain how the insides
work!)


Draw and label the visible

light spectrum with colors and wavelengths.