structural bioinformatics study of amylases, chitinases and cellulases

yokeenchantingBiotechnology

Sep 29, 2013 (3 years and 6 months ago)

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Master

«

Sciences, Technologie, Santé

»

Mention «

In Silico Drug Design

»

2èm
e année



PROPOSITION DE STAGE

Année Universitaire 2012



2013

A envoyer à

Mme Pr Camproux

:

anne
-
claude.camp
roux@univ
-
paris
-
diderot.fr




Nom du Responsable du Laboratoire

ou de l

Entreprise:

Affiliation administrative (CNRS, INSERM,…) et Numéro d’affiliation de l'unité

:

Adresse précise du Laboratoire

:

Head of Biology and Chemistry Department

West University of Timisoara

(Roumanie)


Nom du Responsable de l'équipe d'accueil (EA) :

E
-
mail

:


Nom du Responsable du stage

:

Prof. Dr. Adriana Isvoran

Téléphone

:

+04 0256 592 634




Fax

:





E
-
mail

:
aisvoran@yahoo
.com

HDR

: oui ou non

Ecole doctorale de rattachement

:


Sp
é
cialit
é
du stage

:


Recherche





Professi
onnel


Indiquez par quelques mots clés, l’
orientation scientifique du sujet

:


Titre du stage

:

STRUCTURAL BIOINFORMATICS STUDY OF AMYLASES,
CHITINASES AND
CELLULASES

Ce sujet constitue
-
t
-
il un premier pas vers un travail de thèse : Oui
-

Non

Description du sujet (quelques lignes):

The most abundant organic compounds on earth are cellulose, starch,
and chitin, all of them being
renewable
and
important sources of energy, their digestion in glucose
being

the sub
ject of large amount of
research work.
To valorize the
carbohydrate
-
rich
wa
ste by extracting useful carbohy
drates is still an open
problem with high importance for environmental protection.

Us ua l l y, t he r e a r e t wo c ommon wa ys t o
c onve r t c e l l ul os e, s t a r c h a
nd c hi t i n t o gl uc os e: c he mi c a l a nd e nz yma t i c. Fr om e nz yma t i c vi e wpoi nt,
c e l l ul os e i s de gr a de d by c e l l ul a s e s, s t a r c h i s de gr a de d by a myl a s e s a nd c hi t i n i s de gr a de d by c hi t i na s e s.
Al pha 1
-
4 l i nka ge s ma ke s t a r c h f a i r l y e a s i l y br oke n down by e nz yme s whe r e a s b
eta 1
-
4 linkages result in
linear microfibrils of cellulose which are difficult to break down. In case of chitin, the substitution of the
hydroxyl group by an acetyl amine
one

increases the hydrogen bonding between adjacent polymers and
chitin is
also
dif
ficult to break down.

There are major differences regarding the efficiency of these three
classes of enzymes.


Compari
son of the kinetic parameters reveal that
chitinases and cellulases are less
efficient than amylases.

The aim of this study is to
perform

a bioinformatics analysis in order to identify
sequence and structure similarities/dissimilarities between amylases, cellulases and chitinases with direct
consequences on their catalytic activity a
nd to identify possible actions

to enhance catalytic activ
ity
of
cellulases and chitinases.



___________________________________________________________________
__________________
R
etour par e
-
mail

:
anne
-
claude.camproux@univ
-
paris
-
diderot.fr