pogil 7

wirelessthrillBiotechnology

Dec 11, 2012 (4 years and 8 months ago)

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POGIL 7

biol 212



Genetic engineering


Model 1
, Fig. 18.2 9
th

edition
-


gene splicing





Model 2




Fig. 18.6 9
th

edition.
Plasmids and genetic engineering (genetic
engineering is also referred to as recombinant DNA technology)




Using the diagrams

above, your textbook, and your note set, answer the following
questions.

1.

Model 1 shows
two DNA molecules
.
Point them out with an arrow on
your diagram.


2.

In model 1, pencil in the 5’phosphate and 3’ hydroxyl designations at
ends of the DNA molecules in th
e diagram.


3.

What is EcoR1, and what does it do to a DNA molecule
?





4.

Indicate the sticky ends in model 1with an arrow and label. Why are these
regions of DNA referred to as ‘sticky ends’
?

Add 3’ and 5’ designations to
the sticky ends.












5.

According

to model 1, which enzyme covalently links (or seals) the sticky
ends together
?




6.

In the final step shown by model 1, sticky ends are ligated, or sealed, so to
speak. Chemically speaking, w
hat occurs between the
5’
phosphate and
3’
hydroxyl
during ligatio
n
?









7.

Model 2 shows a DNA sample and
a group of plasmids. What
macromolecule are the plasmids
?





8.

What is the source of the DNA in the DNA sample shown in model 2
?








9.

The second step of model 2 shows that the DNA sample and plasmids
have been ‘cl
eaved’. Which type of protein did this cleaving
?







10.

In the third step, fragments from the DNA sample are joined to plasmids.
Using the knowledge you gained through study of model 1, explain how
this occurs.














11.

Step 4 of model 1 shows a shallow

plate containing bacterial colonies.
How many colonies are on the plate?
What is a colony
?












12.

At the very bottom of model 2, there are five oblong objects containing
plasmid+sample DNA. What are these objects
?

In relation to the plate of
colonies,

where did each of these objects come from?




13.

Are the pieces of sample DNA in each of the
plasmids shown in the last
illustration identical or different
?

How do you know?








14.

What is the purpose of joining the sample DNA to the plasmid and then
introd
ucing it into
E. coli
?

















15.


What are some of the things you might do with the sample DNA now that
you can grow large quantities of it?