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whipmellificiumBiotechnology

Feb 20, 2013 (4 years and 5 months ago)

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Abstract

Background


The use of bioreactors to
combat the growing
problem of greenhouse
gases has been extensively
studied in recent decades.

(Chisti, 2007)

http://www.ieagreen.org.uk/newsletter/dec80/images/biofixation.JPG



The U.S. has
reported a 3.3%
increase of carbon
dioxide emissions in
the past year


Mostly due to car
emissions and
industrial factories.
(Hopwood,

2007)


http://photos.mongabay.com/08/0423methaneglobal.jpg


Knowledge Base

Raceway Pond Design


A raceway
pond is made
of a closed
loop
recirculation
channel

(Chisti, 2007)

Tubular Photo Bioreactor Design


Consists of straight transparent tubes
either made out of glass or plastic (also
known as solar collectors)

(Chisti, 2007)

http://www.agric.wa.gov.au/content/SUST/BIOFUEL/110407_Biod
ieselfrommicroalgae.pdf


Original Design

Control: Airlift Design


A self
-
contained
bioreactor

Taken on:

December 21, 2008

Organism: S.limacinum


Contains pigments
for photosynthesis


Known to contain
EPA, DHA, and
omega
-
3 fatty acids


Reliable source of
oil production for
biodiesels

(Kamlangdee, 2003)

http://roweb.cityu.edu.hk/researchreport/2002
-
2003/Project/020.jpg


Organism: C.reinhardtii


Contains an enzyme
called hydrogenase that
allows creation of
hydrogen

(Tiede, 2008)


Ability to produce
hydrogen under anoxic
conditions

(Fouchard, 2005)


http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii


Magnified 3000X

Literature Review


Molina Grima; et al (2001):


Experiment on tubular photobioreactors using
P. tricornutum


Tested tubular diameter on the amount of sunlight that penetrated through the culture broth


(Najafpour, 2003):


Observed the effect of light intensity, agitation, and liquid dilution rate on hydrogen production


Found use of biocatalyst can be considered alternative to Fischer Tropsch synthetic reactions


Kamlangdee (2003):


Experiment on polyunsaturated fatty acids production by
Schizochytrium sp.


Found single isolate reliable in production of DHA

Purpose

To create a bioreactor design that
would enhance growth rate and
energy yield in
Schizochytrium
limacinum

and
C.reinhardtii

Hypothesis

Modified Design

Taken: January 17, 2009

http://www.agric.wa.gov.au/content/SUST/BIOFUEL/110407_Biodieselfrom
microalgae.pdf


Original Design

Taken: January 17, 2009

Modified Design

Null Hypothesis H(o):

No significant difference
will be found in the growth of C.reinhardtii and
S.limacinum in either bioreactor.

Alternate Hypothesis H(a1):

The airlift bioreactor
will have the most overall growth of biomass

Alternate Hypothesis H(a2):

The growth of
C.reinhardtii and S.limacinum will be greatest in
the Tubular Photobioreactor when exposed to
carbon dioxide.

Discussion


A Tubular Photobioreactor is a suitable
environment for growth of algae


Performance of the Tubular
Photobioreactor surpassed the control
bioreactor


Daily exposure to carbon dioxide did not
greatly effect pH levels in bioreactors


Growth in Tubular Bioreactor was greater
than growth in the Raceway Pond possibly
due to more efficient pump

Conclusion


Data supports the Alternate
Hypothesis


The Tubular Photo Bioreactor
demonstrated a greater amount of
growth as compared to the Raceway
Pond


There was no significance regarding
the Carbon Dioxide levels when
pumped through the Tubular,
Raceway Pond and Airlift
Bioreactors


Limitations


Errors while using the
Spectrophotometer occurred,
causing incongruous data:
Possibly caused by cuvette or
contaimination


Possible errors in GLX Xplorer
readings for carbon dioxide


Possible bacterial contamination
in bioreactors

Future Studies


Revision of Tubular
Photobioreactor and
Raceway Pond designs


Testing various tube
diameters


Using grown C.reinhardtii
and S.limacinum from
bioreactors to extract
hydrogen and oils,
respectively, to test energy
content

Select Bibliography


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-
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