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Tuesday 17 Jan 2006

Lecture 1

Introduction to the biomolecular sciences

Andrew Pearson

BC10M Introductory Biochemistry

Biochemistry arose many years ago as
the application of chemistry to human
physiology, is has grown so large that it
has now spun off many new disciplines.


It now occupies a foundation position
which underpins a large number of
disciplines, following the realisation that
all living things so far discovered all
share the same biochemistry, differing
in only minor details.

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Biotechnology

Microbiology

Forensic science

Molecular biology

Immunology

Food science

Neuroscience

Physiology

Virology

Genetics

Pharmacology

Natural products
biochemistry

Industrial
fermentation

GMO studies

Medical & Clinical
Biochemistry




Biochemical

Sciences

Core


BIOCHEMICAL SCIENCES at MONA


Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

BC10m 2005
-
06.

Timetable

Lectures: Tuesdays 9 am; Thursdays 8 am; Fridays 9 am, Interfaculty Lecture theatre


Week

Date

Event

Title

Tutor

1

Tuesday 17 Jan

Lecture1

Introduction to the biomolecular sciences

Dr Pearson

Thursday 19 Jan

Lecture2

The development of microscopy and cell theory

Dr Brown

Friday 20 Jan

Lecture3

Prokaryotes, eukaryotes, archaea and viruses 1

Dr Brown

2

Tuesday 24 Jan

Lecture4

Prokaryotes, eukaryotes, archaea and viruses 2

Dr Brown

Thursday 26 Jan

Lecture5

Fundamental techniques in microbiology.

Dr Brown

Friday 27 Jan

Lecture6

Quantitative techniques in microbiology

Dr Brown

3

Tuesday 31 Jan

Lecture7

The microbial growth curve in batch culture

Dr Brown

Thursday 2 Feb

Lecture8

Plaque assay; turbid plaque and temperate phage

Dr Brown

Friday 3 Feb

Lecture9

Microbial diversity 1: phylogenetic diversity.

Dr Daniels

4

Tuesday 7 Feb

Lecture1
0

Microbial diversity 2: substrate utilization

Dr Daniels

Thursday 9 Feb

Lecture11

Microbiology applied to the food and beverage
industries.

Dr Daniels

Friday 10 Feb

Lecture1
2

Microbiology applied to chemical & pharmaceutical
industries.

Dr Daniels

5

Tuesday 14 Feb

Lecture1
3

Environmental microbiology: biogeochemical cycles

Dr Daniels

Thursday 16
Feb

Lecture1
4

An introduction to medical microbiology.

Dr Daniels

Friday 17 Feb

Test

Test 1 (Microbiology)


Wee
k

Date

Event

Title

Tutor

1

Tuesday 17 Jan

Lecture1

Introduction to the biomolecular sciences

Andrew Pearson

Thursday 19
Jan

Lecture2

The development of microscopy and cell theory

Paul Brown

Friday 20 Jan

Lecture3

Prokaryotes, eukaryotes, archaea and viruses 1

Paul Brown

2

Tuesday 24 Jan

Lecture4

Prokaryotes, eukaryotes, archaea and viruses 2

Paul Brown

Thursday 26
Jan

Lecture5

Fundamental techniques in microbiology.

Paul Brown

Friday 27 Jan

Lecture6

Quantitative techniques in microbiology

Paul Brown

3

Tuesday 31 Jan

Lecture7

The microbial growth curve in batch culture

Paul Brown

Thursday 2 Feb

Lecture8

Plaque assay; turbid plaque and temperate phage

Paul Brown

Friday 3 Feb

Lecture9

Microbial diversity 1: phylogenetic diversity.

Larry Daniels

4

Tuesday 7 Feb

Lecture10

Microbial diversity 2: substrate utilization

Larry Daniels

Thursday 9 Feb

Lecture11

Microbiology applied to the food and beverage
industries.

Larry Daniels

Friday 10 Feb

Lecture12

Microbiology applied to chemical & pharmaceutical
industries.

Larry Daniels

5

Tuesday 14 Feb

Lecture13

Environmental microbiology: biogeochemical cycles

Larry Daniels

Thursday 16
Feb

Lecture14

An introduction to medical microbiology.

Larry Daniels

Friday 17 Feb

Test

Test 1 (Microbiology)

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

6

Tuesday 21 Feb

Lecture15

The biochemistry of functional groups and bonds

Andrew Pearson

Thursday 23 Feb

Lecture16

Intro. to the common biomols

Andrew Pearson

Friday 24 Feb

Lecture17

Carbohydrate structures and functions 1

Andrew Pearson

Tuesday 28 Feb

Spring Break/Carnival

Thursday 2 Mar

Spring Break

Friday 3 March

Spring Break

7

Tuesday 7 March

Lecture18

Amino acid structures and functions

Andrew Pearson

Thursday 8 Mar

Lecture19

Polypeptide structures and functions

Andrew Pearson

Friday 10 March

Lecture20

Nucleic acid & lipid structures and functions

Andrew Pearson

8

Tuesday 14 Mar

Lecture21

Metabolism and its control

Andrew Pearson

Thursday 16 Mar

Lecture22

Intro. to enzymic catalysis, mechanisms and M
-
M
kinetics

Andrew Pearson

Friday 17 March

Lecture23

Inhibition and regulatory enzymes

Andrew Pearson

9

Tuesday 21 Mar

Lecture24

Carbohydrate fermentations and β
-
ox. of lipids

Andrew Pearson

Thursday 23 Mar

Lecture25

Glucose synth. in plants & liver;
de novo

fatty acid
biosynthesis

Andrew Pearson

Friday 24 Mar

Lecture26

Krebs cycle and integrated carbon metabolism

Andrew Pearson

1
0

Tuesday 28 Mar

Lecture27

Redox reactions, proton pumps & ox. Phos.

Andrew Pearson

Thursday 30 Mar

Lecture28

N
2

& CO
2

fixation; N metabolism & photosyn.

Andrew Pearson

Friday 31 March

Test

Test 2 (Biochemistry)

Andrew Pearson

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

11

Tuesday 4 Apr

Lecture29

Intro. to molecular biology: nucleic acids as genetic
material

Marcia Roye

Thursday 6 Apr

Lecture30

Genome diversity: bacteria, eukaryotes, viruses and
plasmids

Marcia Roye

Friday 7 Apr

Lecture31

The cell cycle, mitosis and meiosis; DNA
replication

Marcia Roye

12

Tuesday 11 Apr

Lecture32

Recombinant DNA technology

Marcia Roye

Thursday 13 Apr

Lecture33

The control of gene expression and transcription.

Marcia Roye

Friday 14 Apr

Good Friday

Marcia Roye

13

Tuesday 18 Apr

Lecture34

Translation and protein maturation

Marcia Roye

Thursday 20 Apr

Test

Self
-
assessment Test (Comprehensive)

Friday 21 Apr

Teaching Ends

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Week

Days

Title

Tutor

1

Mon 16 Tues 17

An introduction to the laboratory and its equipment.

Dr Pearson

Jan

Weds 18 Fri 20

The use of microscopes; examining stained cells

Dr Brown

2

Mon 23 Tues 24

Preparing and sterilizing culture media

Dr Brown

Jan

Weds 25 Fri 27

Culturing microorganisms: (results next day)

Dr Brown

3

Mon 30 Tues 31

Pure culture (results next day) and Effects of temperature
on growth (results 2 and 7 days)

Dr Brown

Feb

Weds 1 Fri 3

Differential stains (results same day)

Dr Brown

4

Mon 6 Tues 7

Environmental Influences: Effect of heat (results after 4
days) Determining numbers of microbes: pipetting and
dilution

Dr Daniels

Feb

Weds 8 Fri 10

Quant. plating methods: spread & pour plates (results next
day)

Dr Daniels

5

Mon 13 Tues 14

Turbidimetric estimation of bacterial growth (results next
day)

Dr Daniels

Labs

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

6

Mon 20 Tues 21

pH meter, amino acid titrations & the Henderson
-
Hasselbalch eqn.

Dr Pearson

Feb

Weds 22 Fri 24

Spectrophotometers and buffers.

Dr Pearson

Mon 27 Tues 28

Spring Break

Mar

Weds 1

ASH WEDNESDAY (no labs Friday either)

7

Mon 6 Tues 7

Gel permeation chromatography

Dr Pearson

Mar

Weds 8 Fri 10

Thin layer chromatography of lipids.

Dr Pearson

8

Mon 13 Tues 14

Transaminations and paper chromatography.

Dr Pearson

Mar

Weds 15 Fri 17

Enzymology lab1.

Dr Pearson

9

Mon 20 Tues 21

Enzymology lab 2.

Dr Pearson

Mar

Weds 22 Fri 24

Lysozyme purification by ion exchange chromatography

Dr Pearson

10

Mon 27 Tues 28

Protein estimation by method of Bradford

Dr Pearson

Mar

Weds 29 Fri 31

Restriction endonucleases and mapping a plasmid. 1

Dr Roye

11

Mon 3 Tues 4

Restriction endonucleases and mapping a plasmid. 2

Dr Roye

April

Weds 5 Fri 7

.

12
April

Mon 10 Tues 11

Final date for handing in lab reports

13

Tues 18 Weds 19

Labs returned and final lab marks posted

April

Thurs 20

Labs returned and final lab marks posted

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

Course learning objectives:



on
-
line at the course website by Friday 20
th

Jan. 2006


Assessment:

Coursework


= 40%







(20% lab reports)







(10% each Tests 1 &2)



Final written exams

= 30% (theory paper)







30% (practical paper)


Since the tests contain theory and practical assessment instruements, you
should be able to see that we place great emphasis on practicals.


If we think that you are not paying sufficient attention to our practicals, you will
fail the coursework component of the course: book
-
work can be done by
anyone but labwork must be instructed.


We intend to teach you valuable laboratory skills; punctuality, attire, report
-
writing and behaviour are all as important as comprehension.

Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson

General points:




you learn more if you are having fun




give us a chance to teach you




you will learn more if you make an effort




we know that many of you will not become



biochemists, microbiologists, molecular



biologists, or biotechnologists




but we hope you will be able to understand


us better afterward taking this course.


Tuesday 17 Jan

Lecture

Introduction to the biomolecular sciences

Dr Pearson