Chapter 9, part B

whipmellificiumBiotechnology

Feb 20, 2013 (4 years and 3 months ago)

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Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings

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Lecture Slide Presentation prepared by Christine L. Case

Micr
o
biology

B.E Pruitt & Jane J. Stein

AN INTRODUCTION

EIGHTH EDITION

TORTORA
• FUNKE • CASE

Chapter 9, part B

Biotechnology and Recombinant DNA

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings

Genetic Engineering

Figure 9.11.1

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings

Genetic Engineering

Figure 9.11.2

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings

Making a Gene Product

Figure 9.12.1

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings

Making a Gene Product

Figure 9.12.2

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings


Used because it is easily grown and its genomics are
known


Need to eliminate endotoxin from products


Cells must be lysed to get product

E. coli

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Making a Gene Product

Figure 9.13

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Subunit vaccines


Nonpathogenic viruses carrying genes for pathogen's
antigens as vaccines


Gene therapy to replace defective or missing genes


Human Genome Project


Nucleotides have been sequenced


Human Proteome Project may provide diagnostics
and treatments

Therapeutic Applications

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Random Shotgun Sequencing

Figure 9.14

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Understanding of
DNA


Sequencing
organisms'
genomes


DNA fingerprinting
for identification

Scientific Applications

Figure 9.16

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Southern Blotting

Figure 9.15.1

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Southern Blotting

Figure 9.15.2

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Southern Blotting

Figure 9.15.3

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Agricultural Applications

Table 9.2

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Genetic Engineering Using
Agrobacterium

Figure 9.18

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Avoid accidental release


Genetically modified crops must be safe for
consumption and for the environment


Who will have access to an individual's genetic
information?

Safety Issues and Ethics