Genetic Technology Study Guide

vinegaryellowwaterBiotechnology

Oct 23, 2013 (3 years and 9 months ago)

95 views

Biotechnology Unit Study Guide



Name ________________________

Chapter 13, lab and internet
activities



Test

Date _____________________



Biotechnology is a field of biology that uses the latest technologies for manipulating and
investigating living organ
isms. Some of the techniques that fall under biotechnology include gene
therapy, DNA fingerprinting, and genetically modifying organisms (i.e., for agriculture). Our
biotechnology unit also afforded us the opportunity to examine some of the ethical consi
derations in
using such technology.



After completing this unit, students should be able to:



Identify some of

the ethical implications of genetic
technology (recall
Gattaca
)



Define
gene therapy

and explain why it is a useful
technique

o

Name some of the di
sorders for which gene
therapy is currently being investigated as a
possible treatment

o

Identify advantages of gene therapy

o

Know
, in general, the process of using a virus as
a
vector

for gene therapy.





Define the term “
genetically modified
” or “
transgenic
” organism



Describe some of the
current
uses of genetically modified/transgenic organisms



Detail the
basic
steps for creation of a
transgenic organism



List the steps of
DNA extraction

and know the
barriers that must be broken through in the
procedures
; u
nderstand how various
chemicals are involved in this procedure



Identify
some common ways that DNA is
obtained from people.



Identify various uses of
DNA fingerprinting



Describe the process and steps of DNA fingerprinting (i.e.,
extraction, making an
agaros
e gel
, loading DNA, gel
electrophoresis
, staining or
radioactive probes
)




Identify the charge on DNA and
describe what happens to
DNA fragments during electrophoresis.



Compare DNA fingerprints and
make conclusions based on the
data.



Explain
restriction enz
ymes

and how they are used in DNA fingerprinting

and/or
other
biotechnology
applications



Relate DNA restriction fragment size to the position of DNA “bands” on a DNA
fingerprint

(i.e.
which fragment in the picture above is largest? smallest?
)



Predict where

a restriction enzyme will cut when given the restriction
sequence
recognized and one or more DNA sequences. Know the #s and sizes of the fragments.



Predict what a gel will look like, based on DNA sequences and restriction
sites/fragments, and probe seque
nces.