1
DR. AMJAD KHALIL
ASSOCIATE PROFESSOR OF BIOTECHNOLOGY
DEPARTMENT OF PHYSICS
Building 6
-
Room 134
Ext: 7152
Course Title: Principles of Biotechnology
(Under Special topics in Physics
(
PHY49
3)
Course Description:
This
course
d
iscusses the fundamental aspects of biotechnology and its importance
to mankind in a concise and lucid explanation of this newly founded science. The course
emphasizes how cell structure and function is a vital starting point for knowledge of
genetic engi
neering and gene technology.
This course explores the fundamental issues of
biotechnology and will have an answer to the main fundamental questions, such as
w
hat
is biotechnology and how did it come about? How is biotechnology done and how is it
being used
today? What are the
consequences of biotechnology applications to the lives
of humans and other animals, plants and to the environment? Finally, what are some of
issues
th
at
biotechnology
raises about the role of science and technology in society
?
Lecture
s will underline the principles and application of Recombinant DNA technology
in industrial, agricultural, pharmaceutical, and biomedical fields.
Bioinformatics and
analysis of biological data using computer software will be introduced.
Course Objective:
1.
To describe the fundamental aspects of biotechnology
2.
To describe the importance of biotechnology to mankind
3.
To underline the principles of Recombinant DNA technology
4.
To describe the applications of microorganisms in industry, environment,
agriculture and
biomedical fields.
5.
To underline the application of Recombinant DNA technology in plant and animal
biotechnology.
6.
Give examples of biotechnology products and biotechnology techniques.
7.
Discuss the importance of transgenic Plants, Bacteria and Animals
.
8.
To def
ine what is bioinformatics and it importance for analysis of data related to
biotechnological applications.
2
COURSE CONTENT
Week
Topics
Chapter in Text
(handout)
1
Modern Biotechnology
What is Biotechnology
Ancient Biotechnology
Classical Biotechno
logy
The Foundation of Modern Biotechnology
1
2
From DNA to proteins
Central Dogma of Molecular Biology
DNA Replication
Transcription & Translation
2
3
-
5
Basic Principles of DNA Technology
Restriction Enzymes, Cloning Vectors and Host Cells
Expressio
n of Cloned Genes
Problems Associated with Gene Expression
Cloning strategies
S
election of cloned Genes
Expression systems
Applications of DNA Technology
Human Therapy, Industry & Agriculture
Environmental Protection
3
6
-
8
Microbial Biotechnology
F
ermentation, Fermentation Systems,
Fomenter Design
Upstream and Downstream Processing
I
-
Production of Whole cell Biomas (starter culture,
single
-
cell protein, bacterial vaccines)
II
-
Micoorganisms and agriculture
Ice
–
Minus Bacteria, Microbial Pesticides
III Products from microorganisms
a
-
Low M.Wt.compounds
1
-
Metabolic End Products
2
-
Essential Metabolites
3
-
Bioconversions
b
-
High M.Wt. Compounds
1
-
Microbial Polysaccharides
2
-
Plastics
5
3
3
-
Proteins
c
-
Fuels
IV
-
Process Dependent on Ge
neral
Microbial
Metabolism
1
-
Bioremediation
2
-
Oil Spills
3
-
Degradation Pathway
4
-
Waste Water Treatment
5
-
Heavy Metals
6
-
Oil and Mineral Recovery
9
-
10
Plant Biotechnology
Plant Genetic Engineering
Applications of Plant GE in:
Corp improvement, Herbicide Resistant, Inse
ct
Resistant, Virus Resistant, Plants as Bioreactors, Risk of
Genetically Modified Organisms (GMOs)
Possib
le Effects of Releasing GMOs into the
Environment
6
11
Animal Biotechnology
Gene Transfer Methods in Animals
1
-
Microinjection
2
-
Embryonic stem c
ell Gene Transfer
3
-
Retrovirus and Gene Transfer
4
-
Transgenic Animals & their applications
5
-
Human Interference in Animal Biotechnology
7
12
-
13
Medical Biotechnology
Gene therapy
Gene Therapy Models
1
-
Liver Diseases (LDLR&FH)
2
-
Lung Diseases (Cystic Fi
brosis)
-
10
13
-
14
Introduction to Bioinformatics in Biotechnology
-
Hand
-
out
Prerequisite: None
Facul ty
Dr.
Amjad Khal i l
4
10
.
Textbook:
Title: Biotechnology, An introduction
Author(s): Susan, R. Barnum
Publisher: Thomson Learning/Thom
son Brooks/Cole USA
Year: 2005
Edition: 2
nd
Book Website:
http://www.biology.brookscole.com
Reference
s
:
The following references are extra
useful re
sources of learning for the students.
1
-
Molecul
ar Biotechnology: Principles and Practices (Hardcover)
by
Channarayappa
(Author)
Publisher: CRC; 1 edition (2007)
ISBN
-
10: 1420051571
ISBN
-
13: 978
-
1420051575
2
-
Biotechnology from A
to
Z, 2nd ed.
ISBN
-
13 978
-
0
-
19
-
963693
-
8
P
ublisher
:
W. Bains, IRL Press at Oxford University Press, 1998
3
-
-
Molecular Biotechnology, Primorse, 1991
4
-
Molecular Biotechnology, Glick & Pasteinak, 1994
5
-
Recombinant DNA, Watson, Witknowski & Zoller, 1993
6.
Environmental Biotechnology, Alan Scragg, 1999
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