Course Title: Principles of Biotechnology (Under Special topics in Physics (PHY49

1

DR. AMJAD KHALIL

ASSOCIATE PROFESSOR OF BIOTECHNOLOGY

DEPARTMENT OF PHYSICS

Building 6
-
Room 134

Ext: 7152



Course Title: Principles of Biotechnology

(Under Special topics in Physics


(
PHY49
3)



Course Description:


This
course

d
iscusses the fundamental aspects of biotechnology and its importance
to mankind in a concise and lucid explanation of this newly founded science. The course
emphasizes how cell structure and function is a vital starting point for knowledge of
genetic engi
neering and gene technology.

This course explores the fundamental issues of
biotechnology and will have an answer to the main fundamental questions, such as
w
hat
is biotechnology and how did it come about? How is biotechnology done and how is it
being used

today? What are the
consequences of biotechnology applications to the lives
of humans and other animals, plants and to the environment? Finally, what are some of
issues

th
at

biotechnology

raises about the role of science and technology in society
?

Lecture
s will underline the principles and application of Recombinant DNA technology
in industrial, agricultural, pharmaceutical, and biomedical fields.

Bioinformatics and
analysis of biological data using computer software will be introduced.


Course Objective:


1.

To describe the fundamental aspects of biotechnology

2.

To describe the importance of biotechnology to mankind

3.

To underline the principles of Recombinant DNA technology

4.

To describe the applications of microorganisms in industry, environment,
agriculture and
biomedical fields.

5.

To underline the application of Recombinant DNA technology in plant and animal
biotechnology.

6.

Give examples of biotechnology products and biotechnology techniques.

7.

Discuss the importance of transgenic Plants, Bacteria and Animals
.

8.

To def
ine what is bioinformatics and it importance for analysis of data related to
biotechnological applications.






2




COURSE CONTENT


Week

Topics

Chapter in Text
(handout)

1

Modern Biotechnology

What is Biotechnology

Ancient Biotechnology

Classical Biotechno
logy

The Foundation of Modern Biotechnology



1

2

From DNA to proteins

Central Dogma of Molecular Biology

DNA Replication

Transcription & Translation



2

3
-
5

Basic Principles of DNA Technology

Restriction Enzymes, Cloning Vectors and Host Cells

Expressio
n of Cloned Genes

Problems Associated with Gene Expression

Cloning strategies

S
election of cloned Genes

Expression systems

Applications of DNA Technology

Human Therapy, Industry & Agriculture

Environmental Protection






3


6
-
8

Microbial Biotechnology

F
ermentation, Fermentation Systems,

Fomenter Design

Upstream and Downstream Processing

I
-
Production of Whole cell Biomas (starter culture,
single
-
cell protein, bacterial vaccines)

II
-
Micoorganisms and agriculture

Ice
–

Minus Bacteria, Microbial Pesticides

III Products from microorganisms

a
-
Low M.Wt.compounds


1
-
Metabolic End Products

2
-
Essential Metabolites

3
-
Bioconversions

b
-
High M.Wt. Compounds

1
-
Microbial Polysaccharides

2
-
Plastics













5


3

3
-
Proteins

c
-
Fuels

IV
-
Process Dependent on Ge
neral
Microbial

Metabolism

1
-
Bioremediation

2
-
Oil Spills

3
-
Degradation Pathway

4
-
Waste Water Treatment

5
-
Heavy Metals

6
-
Oil and Mineral Recovery

9
-
10

Plant Biotechnology

Plant Genetic Engineering

Applications of Plant GE in:

Corp improvement, Herbicide Resistant, Inse
ct
Resistant, Virus Resistant, Plants as Bioreactors, Risk of
Genetically Modified Organisms (GMOs)

Possib
le Effects of Releasing GMOs into the
Environment





6

11

Animal Biotechnology

Gene Transfer Methods in Animals

1
-
Microinjection

2
-
Embryonic stem c
ell Gene Transfer

3
-
Retrovirus and Gene Transfer

4
-
Transgenic Animals & their applications

5
-
Human Interference in Animal Biotechnology




7

12
-
13

Medical Biotechnology

Gene therapy

Gene Therapy Models

1
-
Liver Diseases (LDLR&FH)

2
-
Lung Diseases (Cystic Fi
brosis)

-







10

13
-
14

Introduction to Bioinformatics in Biotechnology
-


Hand
-
out



Prerequisite: None


Facul ty


Dr.

Amjad Khal i l







4

10
.

Textbook:


Title: Biotechnology, An introduction


Author(s): Susan, R. Barnum


Publisher: Thomson Learning/Thom
son Brooks/Cole USA


Year: 2005


Edition: 2
nd


Book Website:
http://www.biology.brookscole.com


Reference
s
:


The following references are extra
useful re
sources of learning for the students.


1
-

Molecul
ar Biotechnology: Principles and Practices (Hardcover)


by
Channarayappa

(Author)



Publisher: CRC; 1 edition (2007)


ISBN
-
10: 1420051571


ISBN
-
13: 978
-
1420051575

2
-

Biotechnology from A

to

Z, 2nd ed.



ISBN
-
13 978
-
0
-
19
-
963693
-
8



P
ublisher

:
W. Bains, IRL Press at Oxford University Press, 1998

3
-

-

Molecular Biotechnology, Primorse, 1991

4
-

Molecular Biotechnology, Glick & Pasteinak, 1994

5
-

Recombinant DNA, Watson, Witknowski & Zoller, 1993

6.
Environmental Biotechnology, Alan Scragg, 1999