Biotech Pre Test

vinegaryellowwaterBiotechnology

Oct 23, 2013 (3 years and 9 months ago)

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Biotechnology

Pre
-
Test



Name ________________________________________


May 11
, 2013


SC.912.L.16.9
Explain how and why the genetic code is universal and is common to almost all organisms.


SC.912.L.16.3 Describe the basic process of DNA replication and
how it relates to the transmission and
conservation of the genetic information
.


SC.912.L.16.10 Evaluate the impact of biotechnology on the individual, society and the environment,
including medical and ethical issues.



_____1.
The job of the lysis buffe
r is to:


A. Rinse any remaining debris from the spin

column.


B. Break open the cell and nuclear membranes to

release DNA.


C. Convert DNA back to its original structure.


D. R
emove DNA from the spin column


http://missionbiotech.com



_____2
) The purpose of DNA extraction is to:


A. Heat the DNA to bind it with RNA.


B. Remove DNA from the patient sample, leaving

only proteins.


C. Isolate DNA strands for use with PCR.


D. Cool the DNA, keeping it in
a dormant state for

later use.


http://missionbiotech.com





_____3
) Which of the following statements about DNA and

RNA is
NOT

true?


A. DNA contains the nitrogenous base uracil;


B. They can both be found in t
he nucleus of a cell;


C. They can both be found in viruses;


D. RNA can be found in ribosomes.


http://missionbiotech.com



_____4) The rate of migration of DNA within an agarose gel during electrop
horesis is primarily based on




what factor??


A. The size of the DNA fragments.


B. The size of the wells in the gel.


C. The volume of the DNA sample loaded


D. The number of DNA fragments.


2012 FL Holt McDougal Biology





_____5) Res
triction enzymes:


A. Restrict the length of a growing DNA strand


B. Determine which nucleotides will be added to a growing strand


C. Cut DNA at specific nucleotide sequences


D. Bind DNA fragments


2012 FL Holt McDougal Biology


_____6) Electrophore
sis can be useful in as a biotechnology tool for:


A. Diagnosis of inherited disorders.


B. Forensic or criminal applications.


C. Developing cures for inherited disorders.


D. All of the above.


2012 FL Holt McDougal Biology


_____
7
) PCR is a techniqu
e used to:


A. Create a master mix for DNA extraction.


B. Replicate DNA strands millions of times (amplify).


C. Convert DNA into RNA.


D. Rinse debris from DNA.


http://missionbiotech.com



_____
8
)

S
hort strands of DNA that attach to specific regions of single
-
stranded DNA are called
:

A.

Fluorescent dye

B.

Nucleotides

C.

Polymerase

D.

Primers


http://missionbiotech.com



_____
9
)
Th
e

enzyme that adds nucleotid
es to a growing strand of DNA in a PCR reaction is called
:


A.

Nucleotase

B.

Polymerase

C.

Primerase

D.

Reverse transcriptase


Florida EOC Coach Jumpstart


_____
10
)
What piece of laboratory equipment would you use to heat and cool reactants for a PCR reaction?

A.

Centr
ifuge

B.

Spin column

C.

Thermocycler

D.

Water bath


F
lorida EOC Coach Jumpstart


_____1
1
) Which of these in NOT an advantage of genetically engineered crop plants?


A. They can produce their own pesticides


B. They can grow larger than unmodified crops


C. The
y cannot cause an allergic reaction


D. They can contain extra nutrients


Florida EOC Coach Jumpstart


_____12
) Consider the following statements about genetic engineering:



A. They can produce their own pesticides


B. They can grow larger than unm
odified crops


C. They cannot cause an allergic reaction


D. They can contain extra nutrients








Which of the statements above are true?

A.

I, II, and IV

B.

I, III, and IV

C.

I and IV only

D.

I, II, III, and IV


Florida EOC Coach Jumpstart




I.

Pieces of DNA f
rom two different organisms can be joined

II.

Genes from complex organisms such as animals can be inserted into simpler organisms
such as bacteria

III.

Recombinant DNA is not passed down to offspring during reproduction

IV.

Genetic engineering can benefit medicine and
society by producing large amounts of
proteins that are used to treat certain diseases.