(Recombinant DNA) Definitions: Genetic engineering - nimitz126

triteritzyBiotechnology

Dec 14, 2012 (4 years and 8 months ago)

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Definitions:

1.
Genetic engineering
-

remaking genes for
practical purposes

2.
Recombinant DNA
-

DNA made from two or
more different organisms

3.
Restriction enzyme
-

enzymes that recognize
short specific DNA sequences and that cut the
DNA there

4.
Plasmid
-

small, circular DNA molecules that
can replicate
independantly

of the main
chromosome

5.
Vector
-

something used to carry the gene of
interest into another cell

1. Cutting DNA
-

restriction enzymes cutout
the gene of interest so there are short,
single strand ends, called
Sticky ends
, on
the DNA



2. Making recombinant DNA
-

the gene of
interest is put into a plasmid that has been
cut the same way.






3. Gene cloning
-

many copies of the gene are
made whenever the host cell reproduces


4. Screening for
transformation
-

scientists look
for a certain
trait to be
expressed in
certain
colonies of
cells following
an attempted
recombination



Screening can
also be done
by using
electrophoresis
and Southern
blot techniques