Lab practical # 2 - South Texas College

tomatoedgeBiotechnology

Feb 20, 2013 (4 years and 8 months ago)

151 views

South Texas College

Division of Math and Sciences

Biology Department

General Microbiology (Biol 2421)

Master Syllabus

/

Tentative Section Outlines

SPRING 2010

________________________________________________________________________


Name:




Dr. Murad
Odeh



Office location:



J
-
3.1118


Office hours:


MON / WED 9
-
10 AM





TUE / THR 830
-
10 AM (online)



Office Telephone:


Office

…..
(956)

872
-
218
7






Fax:




(956) 872
-
2117


Email
:




modeh@southtexascollege.edu


Course Name
:


General Microbiology



Course Time and Location
:


Microb
iology
-

10530

-

BIOL 2421
-

P03

Class

10:00 am
-

12:50 pm

M

South Academic
-
Bldg. J 3
-
208


Lecture


Class

10:00 am
-

12:50 pm

W

South
Academic
-
Bldg. J 3
-
504


Laboratory





Microbiology
-

10531

-

BIOL 2421
-

P04

Class

10:00 am
-

12:50 pm

T

South Academic
-
Bldg. J 3
-
208


Lecture


Class

10:00 am
-

12:50 pm

R

South Academic
-
Bldg. J 3
-
504


Laboratory





Microbiology
-
HYBRID
-

10535
-

BIOL
2421
-

PY1

Class

7:00 pm
-

9:50 pm

W

South Academic
-
Bldg. J 3
-
504

Laboratory

Class

To Be Announced



Internet Classes NT

Lecture

















Textbook
:













Title: Microbiology an Introduction

Author
: Gerard J. Tortora, Berdell R.
Funke, Christine L. Case

Release
: 2009

Publisher
: Benjamin Cummings

Format
: Hardcover 960 pages

ISBN 13
: 9780321550071


Lab Manual



Title: Laboratory

Applications in Microbiology (
A case Study Approach)

Author:
Barry

Chess,
Pasadena Area Community
College

Format
Spiral Bound/Comb,

576 pages

Release: ©2009


ISBN
-
13 9780073375250


Prerequisites:




Biology 2401
with a grade of C or better



Recommended:




Chemistry 1411 and Chemistry 1412

Course Description:




This course is designed to give an overview of the Biology of Microbes, including
Bacteria, Viruses, Fungi, Algae, Protozoa and Helminthes. Information is directed
towards students interested in careers in diverse fields within the Health Sciences,
includi
ng nursing, home economics, dental hygiene, dental and X
-
ray technology,
physician’s

assistant. It is also designed as a class that will fulfill the Microbiology
requirements for food science, environmental monitoring, crop and animal science as
well as Bi
ological science. Wherever possible, new developments in Recombinant DNA
technology/Biotechnology, Virology and Immunology are discussed and expanded upon
in laboratory sessions to provide students with up to date technology as well as a solid
foundation i
n classical Microbiology.

Course Objectives
:



Understand basic information concerning microbial morphology, physiology, genetics,
behavior and ecology.



Describe and analyze interactions between microbes and the host organism in disease
and health states.



Describe and assess the common sources of infection, pertinent routes of entry and
communicability of pathogens.



Evaluate the contributions of microbes
in food production, water contamination,
sewage treatment and soil ecology.



Understand the role of the Immune system in health and disease, and evaluate the
applied uses of serology in diagnosis and research.



Compare the strategies used by bacterial, proto
zoan, fungal, algal and viral pathogens.



Evaluate the effectiveness of a wide range of anti
-
microbial drugs.



In the laboratory, an integrated series of modules will develop an ability to
independently identify unknown bacteria.


Departmental
Course

Goal
s:

To provide students for future academic and work force success by providing instruction. To
provide outstanding educational experience that leads to lifelong learning.


Departmental Course Competencies:




Relate and apply method and appropriate
technology to the study of natural sciences



To identify scientific quantitative methods and the differences between these
approaches and other methods of inquiry



To carry out quantitative methods in a laboratory situation



To demonstrate knowledge of the ma
jor issues and problems facing modern science,
including issues that touch upon ethics and values



To demonstrate knowledge of the interdependence of science and technology and their
influence on, and contribution to modern culture



To provide insight into t
he impact microorganisms have on our daily lives, both positive
and negative



To acquire a knowledge of microbial mechanisms of pathogenicity






Learning Objectives:




The instructor will include a variety of learning activities that are

designed to engage the
student

on task.



Learning activities include: class participation during the lectur
e and lab with class
discussion

relevant to the material presented.


Core Course Objectives
with

SCANS:


Course Learning Outcomes (CLOs):




To int
roduce students to the development of microbiology as a science.



To provide students with a basic understanding of the chemistry underlying the field of
microbiology.



To introduce students to various procedures and tools used to observe microorganisms.



To
introduce students to the functional anatomy of prokaryotic and eukaryotic cells and
viruses.



To introduce students to all major aspects of microbial metabolism and energy
production.



To provide insight into the impact microorganisms have on our daily live
s, both positive
and negative.



To inform students of the nature and importance of the techniques used to isolate,
culture,

observe, and identify microorganisms safely.



To introduce students to the various control procedures employed in the control of

microorganisms, and the reduction of their detrimental effects.



To acquaint students with the genetics of microorganisms and their importance to the
fields of

biotechnology and recombinant DNA technologies in general.



To introduce students to the taxonomy
of microorganisms, and various methods of
identifying and classifying them into groups.



To enlighten students as to microbial mechanisms of pathogenicity and their
relationship to disease and epidemiology.



To allow students to u
nderstand the role of the no
rmal and abnormal immune system in
health

and disease, and to evaluate the applied uses of serology in diagnosis and
research.



To e
valuate the effectiveness of a wide range of anti
-
microbial drugs and learn their
effects and possible side effects on pathog
en and host.



Allow students to describe and analyze interactions between microbes and the host
organism in

disease and health states on a system
-
by
-
system basis.



To provide information about the ways in which microorganisms interact with their
environment
and the practical consequences of these interactions including microbial
roles in biogeochemical




Basic Skills:




(Reading, writing, mathematics, arithmetic, speaking and listening)
.



Students are required to read the textbook for the instructor to teach the concepts that
the
c
ourse

encompasses.



Students will take lecture notes and ask necessary questions. The



I
nstructor will

insure that the students understand the concepts by providing examples
that are relevant to the topic and the student and by asking the students questions.
Upon completion of the course, the student will:

o

H
ave

students

read the required chapters and
related material,

o

H
ave a basic understanding of the topics, and

o

A
pply the concepts to everyday life.



Thinking Skills:




(Learning and Reasoning)

The student will be able to demonstrate an understanding of
concepts posed by the instructor. The questions

are related to the concepts that are
being taught in the course. The answers will require critical thinking skills that inc
lude
reasoning. The instructo
r

will provide the necessary prompts to insure student success.


Technology:




(Computer and scientific apparatus)
Students will have access to the Internet. Students
will utilize scientific tools and methodology.


Personal Qualities:




(Responsibility
, self
-
esteem, sociability, self
-
management, integrity and honesty).
The
student m
ust exert a high level of effort and persevere toward goal att
ainment.
Students must believe
one’s own self
-
worth and maintain a positive view
of one
-
self.
Also, demonstrate

understanding, friendliness, adaptability, empathy, and politeness in
-
group
settings. Stu
dents

must assess one
-
self accurately, set personal goals, monitor
progress, exhibit self
-
control, and choose ethical

courses of action.


Departmental Course Grade Criteria

Basis for Course Grades:



Your course grade will be determined from
a class
-
wide point distribution. The general
distribution of grades will be as follows:

90
-
100 (A)
/ 80
-
89

(B) / 70
-
79 (C) / 60
-
69 (D) /
< 60 (F)



There are 5 scheduled lectures Exams through the course of the semester. Each Exam is
worth 100 pts.

o

Each
Exam will be composed of roughly 50 multiple
-
choice questions. The
number of exams that would be counted toward your final grade is 4. Total of
400 pts.

o

Eleven multiple choice questions worth 10 points each posted on WebCT.
Please observe the date’s ava
ilability for each quiz.



Two
-
Three lab practical exams will be given during the normally scheduled lab period.
Content of the lab practical exam will be discussed at a later time.


Class
-
wide evaluation:

A. Five lecture exams (100 pts. each)………

400 points

possible

B. Eleven Lecture quizzes (10 pts. each)

OPTIONAL
*


Up to 20 points

C. Two
-

Three lab practical exams………..

250 points possible

D
*. Attendance / Participation / Observations……

5
0 points possible

Total Points ………………………………………….

4
00 P
oints
possible for lecture

300

Points

for laboratory.

*Quizzes are optional and considered to be extra credit. To access quizzes please log in to
blackboard using the following link
http://ecourses.southtexascollege.edu/webct/logonDisplay.dowebct

REGULAR CLASS: Lecture portion 70%..... Lab portion 30% of the total grade

HYBRID ONLINE CLASS: Lecture portion
60%..... Lab portion 40% of the total grade

NOTE:

THE LOWEST GRADE OF ANY OF THE LECTURE EXAMS
EXCLUDING THE FINAL EXAM

SCORE WILL BE DROPPED

The evaluation of your lecture / laboratory work will include observations of the following:



Any lecture / exercise not performed



Arrival to class
on time




Preparations for the exercises (pre
-
labs)



Microbiological technique and organization



Sharing of work with lab partner



Demonstrated interest in the subject



Carefulness in working



Quality o
f data you obtained

Attendance Policies



Attendance will be recorded at every lecture and lab and will be taken into
consideration when determining the student's participation grade.


One unexcused
absence will not affect your grade.


Two or more
unexcus
ed

absences will lower your
course grade.



Students are required to attend one scheduled laboratory periods each week (2 hours
and 50 min.) as well as one 2 hours and 50 min minute lecture session.


It is important
to
arrive on time

in order to receive all

pertinent information.



Students are expected to spend on average no more than 6 hours total per week in the
BIO 2421



Students who provide acceptable written, dated documentation of
personal
emergency, confining illness, religious conflict
in a timely fash
ion

may be allowed to
make up missed work for credit. Documentation must include relevant names, times,
dates and an explanation of the date(s) and reasons(s) for absence.



Students who have advanced knowledge of events that will conflict with BIO 2421 must

present acceptable documentation of such events prior to their absence.



Make
-
up labs are not guaranteed to be available
, as there are time constraints involved
in several of the lab set ups as well as a limit to the number of students involved in the
lab
exercises. If you have an acceptable reason for absence we will make every effort to
accommodate you.



There will be no make
-
up quizzes given. Students will however be allowed to drop their
two lowest quizzes



Students who wish to have consideration for rel
igious observances which conflict with
labs, exams or quizzes must present verification in writing to Dr. Odeh within one week
of the first lecture. If the religious observance is such that the exact date cannot be
determined, contact Dr. Odeh within the f
irst week of classes and inform him about the
approximate date or dates of absence(s).



Class absences before and after vacations (
e.g.

Thanksgiving Break, Spring Break) are
not excusable, except as aforementioned.

Developmental Studies Policy Statement:


The College’s Developmental Education Plan requires students who have not met the
college
-
level placement standard on an approved assessment instrument in reading,
writing, and/or mathematics to enroll in Developmental Studies courses including
College Suc
cess. Failure to attend these required classes may result in the student's
withdrawal from ALL college courses.



Statement of Equal Opportunity:

No person shall be excluded from participation in,
denied the benefits of, or be subject to discrimination und
er any program or activity
sponsored or conducted by South Texas College on the basis of race, color, national
origin, religion, sex, age, veteran status or disability.




Alternative Format Statement:

This document is available in an alternative f
ormat
upon

request by calling (
956)
872
-
2023
.




A
DA Statement:

Individuals with disabilities requiring assistance or access to rec
eive
services should contact DIS
ABIL
ITY Support Services at (956
) 872
-
2173.






















DATE

LABORATORY EXERCISES

LECTURE TOPICS/CHAPTERS

WEEK 1

(January 19
-
22)

Exercise 1 : SAFETY; P 1

Remaining Period could be used for lecture
material…
History and scope of Microbiology (1)

Class Introduction/Handouts

The microbial world and you (1)

WEEK 2

(January 25
-
29
)

Exercise 7 Aseptic Techniques ad pure culture
techniques P61 and P67

Exercise 3 Ubiquity of Bacteria P41

Exercise 2 microscopy P9
-
20

Appendix C Preparation of culture media P553


Chemical
Principles (
2)


WEEK 3

(Feb 1
-

5
)

February 3
rd
: 12
th

day of
class

Exercise 43 Wet mount Hanging Drop Slide
P343

Exercise 37 Smear Preparation and Simple Stain
P315

Environmental conditions



Exercise

14 Temperature P119,



Exercise

15 Ph P127



Exercise

16 Osmotic Pressure P127


Observing microorganisms
through a
microscope (3)


WEEK 4

(February 8
-
12

Exercise 45 Streak Plate P351

Exercise 38 Negative Stain P321

Exercise 40 Gram Stain P329


Functional anatomy and prokaryotic and
eukaryotic cells (4)

WEEK 5

(February 15
-
19

)

Exercise 42 Acid fast stain
P339

Exercise 41 Endospore stains P335

TEST # 1(chapters 1, 2, 3, 4
)

Microbial Metabolism (5)

WEEK 6

(February 22
-
26
)

REPEAT

Exercise 40 … Gram stain P329

Exercise 39 Capsule stain P325

Selective and Differential Media



Exercise 53 EMB P391



Exercise 49 MSA P375



Exercise 50 MacConkey P 379



Exercise 56 blood Agar P 403

Microbial Metabolism Cont’d

Microbial Growth (6)

WEEK 7

(March 1
-

5
)

Exercise 13 Cultivation of anaerobes P111

Exercise 89 Quantitative Techniques: viable
plate Count P537

Exercise 64 Carbohydrates Fermentation
(phenol red broth) P435

Exercise 60 Triple sugar Iron agar P419


The control of microbial growth (7)

WEEK 8

(March 8
-
12
)

LAB PRACTICAL #1

Microbial Genetics

cont’d

TEST #2 ( Chapters 5, 6, and 7)

Microbial genetics (8)

WEEK 9

(March 15
-
19
)


SPRING BREAK

SPRING BREAK

WEEK 10

(
March 22
-
26
)

Exercise 76 Starch hydrolysis P481

Exercise 79 Caseinase P493 Exercise 80
Gelatinase P497

Biotechnolgy and Recombinant DNA
technology (9)


WEEK 11

(March
29
-

April2
)

Exercise 67
Catalase P447

Exercise 81 DNase P501

Exercise 68 Oxidase P451

Exercise 58 (SIM) P411

Anti
-
microbial drugs

(20)


Test #3 (Chapters 8,9,20
)


May 8
-
14 (Final
s
). Chapters and specific Exam date TO BE ANNOUNCED































Exercise 74Phenylalanine Deamination P473

WEEK 12

(April 5
-
April 9
)

Lab practical # 2

Eukaryotes (12)

Viruses, Viroids, and Prions (13)

WEEK 13

(April 12
-
16

IMViC Test P 411, P443, P461

Exercise 27 Water analysis Phase I Lauryl sulfate
tubes inoculation P221


Mechanism of Pathogenicity (15)


WEEK 14

(April 19
-
23
)

April 20 Last Day to
withdraw

Exercise 20 Kirby Bauer Antimicrobic sensitivity
P163

Exercise 27
Water analysis(Phase II and III)
Inoculation of Brilliant Green tube & EMB
plates P221

EXAM # 4 (chapters 13, 15
)



WEEK 15

(April 26
-
30
)

Exercise 62 Litmus Milk P427

Exercise 69 Nitrate Reduction P455

Exercise 78 Urease Test P489

Exercise 27 Water Analysis (III) Results

Innate
-

Non
-
specific immunity (16)


WEEK 16

(May 3
-
May 7
)

LAB PRACTICAL # 3

Adaptive
-

specific Immunity (17)

May 8
-
14 (Finals).Chapters and
exact
final
e
xam date

and time…

TO BE ANNOUNCE