Study Guide for Week 10 – Genetic Engineering - ASU West

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Dec 11, 2012 (4 years and 8 months ago)

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Study Guide for Chapter 10


Genetic Engineering


1. For the following techniques/procedures know why they are used, the mechanics of
how they work, and how the results are interpreted (if it is an analytical technique).





Standard Gene Cloning



DNA Sequen
ce Analysis



Southern Hybridization



Polymerase chain Reaction



Gel electrophoresis



Restriction endonuclease digestion



microarray analysis



2. Be able to name 4 commercial products that are a result of genetic engineering of
microbes.


3. What is a general

pathway to get a commercial drug overexpressed in bacteria.


4. What is a GMO?


5. Be able to name 4 GMO’s that include a bacterium, a plant, and an animal.


6. How are plant GMO’s made?


7. How are animal GMO’s made?


8. What is the difference and s
imilarities between gene therapy, antisense RNA, and
triplex DNA?


9. Why is the combination of genome mapping and microarray analysis such a powerful
technique with so much promise?


10. Know four products that are made through recombinant DNA technolo
gy.


11. Words to know:


Reverse transcriptase

Ligase

Palindrome