SAFETY GUIDELINES AND INSTRUCTIONS

taxmanstrongBiotechnology

Dec 11, 2012 (5 years and 26 days ago)

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SAFETY GUIDELINES AND INSTRUCTIONS

FOR LABORATORY WORK IN GENETIC
ENGINEERING

according to Section 2 Para. 2 GenTSV


Containment Level 1


Affected Area:

Institution, Address, Department, Room Numbers


(bitte oben die entsprechenden Angaben einfügen)




Roo
ms are labeled with a sign indicating installation to con
-




duct genetic engineering and biotechnology laboratory work


Dangers for Laboratory Workers, Population and Environment

Section 7 GenTG : "Containment Level 1 is relating to laboratory work in ge
netic
engineering which, according to the current knowledge of science, does not constitute any
risk for human health and environment." The installation for genetic engineering laboratory
work is used to process recombinant eucaryontic cell lines as well a
s recombinant E. coli K12
derivatives classified at Containment Level 1
(ggf. an die tatsächlichen Arbeiten
anpassen)
. According to the current stand of science these organisms do not constitute
either risk or danger for man or environment.


Precautions an
d Safety Instructions




Eating and Drinking



Lab Coats



Smoking

Prohibited!




Mandatory!



Prohibited !


Any work in the laboratory requires approval by the project supervisor. Personnel performing
genetic engineering procedur
es must be qualified and adequately instructed by the project
supervisor. Personnel must be lectured on possible hazards of the respective work place on an
annual basis. In addition to that female personnel must be advised on possible risks for
pregnant wo
men in particular with regard to hazardous material handled by them.
Participation in such instruction sessions requires written confirmation. Laboratory work in
genetic engineering is subject to the keeping of appropriate records.


The rules of good labor
atory practice when working with
microbiological material are to be adhered to:




When work is in progress, laboratory doors must be closed.



Use personal protective equipment! Wear laboratory coats or gowns, disposable gloves
where required, and always prot
ective goggles at the remotest risk for the eyes.







Eating, drinking, smoking, snuffing, applying of cosmetics and storing of food, drinks
and tobacco are absolutely prohibited in the laboratory!



Mouth pipetting is prohibited! Mechanical pipetting instrument
s should be used instead.



Minimize the generation of aerosols.



Use syringes and cannulas only if absolutely necessary.



Personnel wash their hands after finishing work.



Keep laboratory tidy and clean; only instruments and material actually required in the
w
ork process are to be placed on work benches; keep stocks of regulated material in
separate storage rooms or cabinets, if possible.


Transport and Waste Disposal

Transport of genetically modified organisms between genetic engineering installations in
break
-

and leakproof containers only.
All microbiological waste must be autoclaved or
properly inactivated before
disposal
(
bitte hier den Standort des Autoklavenraums angeben
)

Observe autoclave operating instructions! Test working order safety of autoclave wit
h spore
sample on a six
-
monthly basis, recording results.


Incident Regulations



Disinfect contaminated work surfaces and instruments (e.g. with a solution of 70%
ethanol). Remove more substantial spills with cellulose or similar material prior to
disinfec
ting, and autoclave wiping material subsequently.



Disinfect contaminated clothing or skin with alcoholic disinfectant.



Rinse eyes and mucouse membranes profusely under running water.



Treat injuries immediately under first aid conditions, if possible (if re
quired, apply
disinfectant).



Report all injuries to the laboratory supervisor without delay. See a physician.



Observe fire protection rules and evacuation plans; familiarize with these manuals to
prevent unnecessary delays in incidents of emergency.






Fire Brigade :






Emergency call






Laboratory Supervisor :




Safety/Security Manager :

PD Dr. E. Kaufmann


23886

Facility Doctor :

Arbeitsmedizinische
Untersuchungsstelle


6619
0


For any questions pertaining to relevant statutory regulations

covering biological safety etc. please contact the

Commissioner for Biological Safety

PD Dr. E. Kaufmann


Phone: 238
86

e
-
Mail: eckhard.kaufmann@uni
-
ulm.de