MCB 212/326 Genetic Engineering and Functional Genomics ...

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Dec 11, 2012 (4 years and 10 months ago)

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MCB 212/326
Genetic Engineering and Functional Genomics
Syllabus

Week:
I. Jan 18: Introduction-Genetic Engineering and a tour of Genome Space
Jan 20: DNA/RNA Processing and Gene Expression (a review): Chpt. 3

II. Jan 25: Basic Techniques of Molecular Biology
Jan 27: Restriction Endonucleases, Vectors, Cloning: Chpt. 4

III. Feb 1: Library Screens: Chpt. 4
Feb 3: PCR, Sequencing: Chpt. 5

IV. Feb 8: Prokaryotic Gene Expression: Chpt. 6
Feb 10*: EXAM I
*Assignment #1 due (MCB 212 and MCB 326)

V. Feb 15: Prokaryotic Gene Expression: Chpt. 6 cont’d &
Eukaryotic Gene Expression: Chpt. 7
Feb 17: Genetic Engineering in Plants I: Chpt. 17
VI. Feb 22: 10. Genetic Engineering in Plants II: Applications: Chpt. 18
Feb 24: 11. Genetic Engineering in Plants III: the Next Generation of Rice

VII. Mar 1: 12 & 13a Sequence Analysis, Genome Structure
Mar 3*: 13a and 13b. Comparative Genomics
*Assignment #2 due (MCB 212 and MCB 326)
VIII. SPRING BREAK:
IX. Mar 15: 14. Functional Genomics: Analysis of Gene Expression
Mar 17: 15. Modifying Gene Expression and Cellular Function

X. Mar 22: EXAM II
Mar 24: OPV and the emergence of AIDS

XI. Mar 29: The Origin of AIDS, con’t; vaccine intro; Edible vaccines
Mar 31: vaccine targets: malaria and ebola
XII. April 5: Human Gene therapy I: Chpt. 10
April 7*: Human Gene therapy II: examples
*Assignment #3 due (MCB 212 and MCB 326)

XIII. April 12: Genetic engineering in animals I: Cloning Chpt. 19
April 14*: Genetic engineering in animals II: Knockouts and Knockins,
Inducible GeneTargeting

XIV. April 19: Genetic engineering in animals III: examples
Genetic engineering in animals IV: xeno transplantation part a
April 21: Genetic engineering in animals IV: xeno transplantation part b

XV. April 26: Ethics and Patent Law Guest Lecture
April 28*: EXAM III
*Assignment #4 due (MCB 212 and MCB 326)



FINAL EXAM May 5 8am
Required Textbook: (can be found in the UCONN COOP)
Molecular Biotechnology: principles and applications of recombinant DNA

B.R. Glick and J.J. Pasternack, 3
rd
edition 2003

Supplemental Reading: (on reserve in Babbidge Library)
Principles of Gene Manipulation : an introduction to genetic engineering

Old, RW and Primrose, S.B., 1985

An Introduction to Genetic Engineering

D. Nicholl, 2002

Other good texts: (can be purchased from publisher)
A Primer of Genome Science

G. Gibson and S. Muse, 2002

From Genes to Genomes

J. Dale and M. von Schantz