Genetic Engineering Paper Lab - Biology

taxmanstrongBiotechnology

Dec 11, 2012 (4 years and 8 months ago)

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Genetic Engineering
Simulation


Name:_____________________

Class:_________


Date:____________



Part One: PLASMID CONSTRUCTION


1.

From the Plasmid sheet cut the strips along the lines and tape them together in any
order. You may use from four to six stri
ps for your plasm
id. Make sure you have an
ORI
site!

2.

Tape the two remaining ends together to form a circular shape.

Why are the two ends
taped together? ______________________________________________________.

3.

On the back of the plasmid identify and label

each antibiotic resistance area and the
replication area (ORI).

4.

Cut the nine restriction enzyme cards apart from the Restriction enzyme sheet.
Compare the sequences of base pairs on the cards with your plasmid. Mark on your
plasmid where each restrict
ion enzyme would cut. Depending on your plasmid you
may find that you cannot use all the restriction enzymes.

5.

On the back of this sheet draw a diagram or map of your plasmid, a big circle will do.
Mark the antibiotic sites, restriction sites and the repl
ication origin on you r diagram.
This has become routine for scien
tist to describe and compare different pieces of DNA.


Part Two: DNA CONSTRUCTION


1.

Cut out the strips from the DNA sheet. Tape the DNA together in order of one to six
with strip one on to
p and six as the bottom strip.

2.

What does the first and last codon code for? First__________ Last__________

3.

Determine and mark which restriction sites are near the protein gene that you will
transfer.


Part Three: RESTRICTION AND INSERTION

1.

Compare the re
striction enzyme from the DNA strip with the location of the same
restriction enzyme on you plasmid. Using scissors cut both the DNA strip and your
plasmid at the appropriate locations.

Which restriction enzyme will you use _______
_

2.

Match the sticky ends

of the plasmid and the gene you are inserting and Ligase (tape)
the ends together.
What is Ligase is real gene splicing?_________________________

______________________________________________________________________

3.

In a real genetic lab the recombinant

DNA plasmid would then be inserted into a new
bacterium and grown in a culture dish. The bacteria grown will then have to be
screened
to determine if the transfer was successful. To screen for a Genetically
Modified Organism (GMO) the bacteria cultures
will be treated with an antibiotic that
will kill all the bacteria except the GMO bacteria. Which antibiotic would you apply to
the bacteria to determine if your genetic transfer
was successful? __________________

Explain why you choose this antibiotic.



______________________________________________________________________


______________________________________________________________________



Questions to ponder


1.

What are the beneficial points of genetically engineering:


a.)

Ba
cteria that can produce insulin.



b.)

Bacteria that can produce the human growth hormone.



c.)

Organisms that can glow

under UV light.



2.

What are possible ethical issues for engineering:



a.)

Organisms that glow under UV light.



b.)

Genetically modified food.



c.)

Human
growth hormone.


3.

Should people be concerned about engineering crops to withstand frost, insect pest,
or preventing fruit from spoiling to fast?


4.

If herbicide resistance is engineered into crops how might this affect the farmers,
other plants, ground wate
r supplies?


5.

What are the risk in eating
genetically
engineered food?


6.

Would you knowingly eat gen
etically modified food? Why or Why not?


7.

Have you?