Introduction to Mildew Mania Plus 2013

tanktherapistBiotechnology

Oct 23, 2013 (3 years and 7 months ago)

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Mildew Mania
PLUS


An evolutionary arms
-
race in the field


2009 mildew sampling pathotypes

Red = wildtype

Blue = fungicide resistant mutant


2012 mildew sampling pathotypes

Red = wildtype

Blue = fungicide resistant mutant

AIMS


To
encourage

students to enrol in
agricultural studies and engage with
scientific research



To identify
when

powdery mildew is present



To collect samples and identify
which

barley
varieties have genetic resistance, and
which strain(s) of powdery mildew are
present


Continue
planting
1 pot
each
week
until signs
of
mildew
infection.

Week 1:
plant

Baudin
seeds

Week 2:
plant
B
audin
seeds

Week 3: plant
B
audin seeds

1

1

2

1

2

3

Week 4: plant
B
audin seeds

1

2

3

4

3

5

6

7

8

9

Week 5: INFECTION FOUND??!
Proceed with planting all extra
cultivars including Baudin with
and without fungicide

Monitor new pots
every week for
signs of infection.
Record how
many, in which
pots and when
infection occurs


send samples to
ACNFP

4

2

1

10

11

All pots should contain ~4
seeds/pot, ~1cm deep
,
and watered
every
2
-
3days

week

Activity for the week

1

Plant pot 1 of Baudin seeds

2

Plant pot 2 of Baudin seeds, check pot 1 for mildew
, record
results

3

Plant pot 3 of Baudin seeds, check pots

1 & 2, record results

4

Plant pot 4 of Baudin seeds, check pots 1, 2 & 3,

record results.

G
ive pot 1 fertiliser

5?

POWERDRY MILDEW FOUND?! Take photo and email/text to

ACNFP to confirm. Plant pot 5 of Baudin seeds. Plant pots
6,7,8,9,10&11 with other barley cultivars, fungicide treated
Baudin

and wheat. Give pot 2 fertiliser.

6

Give pot 3 fertiliser. Check new pots for mildew, record results
(if mildew present, if so, which plants?)

7

Give pot 4 fertiliser. Check new pots for mildew, record results

8

Give pots 5,6,7,8,9,10 &11 fertiliser. Check new pots for mildew,
record results

9

If any pots other than the normal Baudin get infected, collect

a sample and post it to us for testing.

Advantages of the PLUS


More chance of infection due to presence of
“disease nursery”



Multiple cultivars of barley with different levels of
genetic resistance



Genetically susceptible barley treated with
fungicide



Extension lesson activities which we can design
and present to your students


Possible incursions:


All years


Experimental design and practise


how to ensure a fair and
reliable test


Year 8


Classification of cells
-

difference between fungi, plant and
animal cells: physical properties and reproduction


Year 9


Ecosystem communities


biotic and abiotic factors which
effect agricultural populations
eg
. competition, genetic
variability, rainfall, fungicide use etc


Year 10


Co
-
evolution


mildew and barley have evolved together, as
one resistant gene evolved, a new virulence gene evolved


Year 11&12
(biology/ integrated/plant production systems)


Using biotechnology
-

students design a diagnostic test to
determine if a specific barley cultivar contains a mutation in a
resistance gene