BIO 304: General Genetics, Fall 2003

stubbornnessuglyBiotechnology

Dec 12, 2012 (4 years and 11 months ago)

150 views

BIO
L 377
:
Special Topics:
Molecular

Genetics
,
Spring

2011


Instructor:

Dr. Kathy Szick
-
Miranda

Office:

Sci I

316




Phone:

6
5
4
-
6165

Email:

kszick
-
miranda@csub.edu


Web:

www.csub.edu/~kszick_miranda

Office
hours:

M
1
1:0
0
-
12:3
0
; T 12:30
-
3:00

Lecture:

M W

F

9:30
-
10:5
0a
m
, Sci

I
I 343



Required
Book
s
:


Weaver, R. 2008
.
Molecular Biology
, Four
th Edition.

McGraw Hill
.


Discussion papers

-

W
ill be available via the web
.


Course Description:


Evolution and
molecular organization of the cell, macromolecules of organisms, and gene expression.
Emphasis placed on recombinant DNA techniques, genetic engineering and biotechnology.

Prerequisite:
BIO 304.


Course
Goals:

In this course we will examine
the core conce
pts in

molecular

genetics
, including DNA structure,
replication and repair,
gene expression,
regulation of gene expression

as well as topics involved in
biotechnology
. Upon completion of this course, you
are expected to
be able to:



Define and
describe
the chemical

structure of both DNA and RNA at the molecular and macromolecular
level.


Describe the process of replication including enzymes involved
.


Describe transcription and translation in both prokaryotes and eukaryotes
.


Understand how and why
mutations occur, what mechanisms are involved in repair of mutations and
what effect mutations have in the short term and evolutionarily.


Finally, an emphasis will be placed on the ability to i
nterpret data that is the direct result of recombinant
DNA tec
hniques

and procedures
.


Academic Policies:

It is expected that your work is YOUR work. In the unexpected event of plagiarism
and/or dishonesty during exams or for the research term
-
paper, the student will receive a zero on that
activity and be referred to

the campus administration for disciplinary action.
Please refer to the campus
2009
-
2011 catalog page 82
.


Attendance and Participation
:


I will not record attendance
however,
the lectures will provide a great deal of information that you may
not find in your text.
Your overall grade in the course will reflect the extent to which you participate over
the course of the quarter.


Grading:

Grades will be based on the percentage of
points accumulated in the course (lecture and lab)
using the scale shown below.


A = 94
-
100


B
-

= 80
-
82.9


D+ = 67
-
69.9



A
-
= 90
-
93.9


C+ = 77
-
79.9


D = 63
-
66.9


B+ = 87
-
89.9


C = 73
-
76.9


D
-

= 60
-
62.9


B = 83
-
86.9


C
-

= 70
-
72.9


F = 0
-
59.9

*All assignmen
ts are due at the beginning of class on the date indicated. Any assignment turned in after
that time will be penalized 10% per day.


Assignment Point Values:

Exam I





100pts

Exam II





100pts

Exam

III





100
pts

Exam IV (Final)




100pts

Independent St
udy Topic Write
-
up




50pts

TOTAL





350
pts
*


*I will only count your 3 highest exam scores in your final grade calculation.


Reading

Assignments
:



1. For each

lecture
, the reading will relate to the topic of that day and should be done in advance of
lecture so that you will be familiar with what will be lectured on.


2. There may be additional handouts and/or reading.


Independent Study Assignment
:

You assignment is
to f
ind an article

in some area of molecular biology that interests you

(some examples
of appropriate
journals

are
:
Science;

Nature
;
Cell
;
Plant Molecular Biology
;
The Plant Cell
;
Proceedings
of the National
Academy of Sciences, USA
;
Molecular and General Genetics
;
EMBO J. (European
Molecular Biology Organization Journal)
.

Once you have selected
an article you must get it approved by
me.
Submit a PDF file

of the complete article to me
for approval (see syllabus for deadline). This can be
sent by e
-
mail. Please send only one article at a time for approval.
Once you have received approval, you
are t
o prepare
a detailed description of one of the molecular techniques utilized in the journal article.
Your objective is to prepare a powerpoint presentation that could be used to teach a class about how this
technique works and the type of information that
can be gained from running this type of experiment.
Be
sure to include the following:


1.

The powerpoint presentation (hard copy and electronic copy).

2.

Description of the experiment along with results


Y
ou have conveyed a good
understanding of how the techniq
ue was performed and what type of in
formation was
gained using this technique
.

3.

Complete reference section.


Lecture Schedule BIOL 377 Spring 2011

WK

Day/Date

Topic

Reading




1

M
-
Mar 28

Intro to course

Ch1 1
-
11;Ch2 13
-
15;Ch3 32
-
50


W
-
Mar 30

DNA
Structure

Ch2 15
-
30




F
-
April 1

Independent Study Topic





2

M
-
April 4

Guest Speaker






W
-
April 6

DNA Structure




F
-
April 8

Chromosome Structure

Ch13 359
-
367; 377
-
383



3

M
-
April 11

Replication

Ch20 639
-
659




W
-
April 13

Exam I






F
-
April 15

Replication

Ch21 683
-
704




4

M
-
April 18

Eukaryotic Replication





W
-
April 20

DNA Damage and Repair

Ch20 660
-
682





F
-
April 22

Independent Study Topic





5

M
-
April 25

DNA Damage and Repair





W
-
April 27

Transcription (prokaryotes)

Ch6 126
-
165




F
-
April 29

Trans
criptional Regulation (Proks)

Ch7 172
-
183

6

M
-
May 2

Exam II





W
-
May4

Transcription Eukaryotes

Ch10 250
-
274; Ch11 281
-
314




F
-
May6

Transcript Processing

Ch15 444
-
464




7

M
-
May 9

Splicing

Ch14 399
-
443




W
-
May 11

Transcriptional
Regulation (Euks)

Ch12 321
-
346


F
-
May 13

Translation

Ch19 607
-
633; Ch17 521
-
546



8

M
-
May16

Translation

Ch18 573
-
599





W
-
May 18

Exam III





F
-
May 20

Translation




9

M
-
May 23

The Genetic Code

Ch18 564
-
571





W
-
May 25

Molecular Tools

CH 5 84
-
119





F
-
May 27

Cloning/Biotechnology

Ch24 770
-
777



10

M
-
May 30

HOLIDAY






W
-
June 1

Biotechnology






F
-
June 3

Genomics and Proteomics

CH 24 797
-
827



10+1

M
-
June 6

Genomics and Proteomics






W
-
June 8

Final 11
:00
-
1:30









*
A
rticle selections

due Monday April 4th
,
project
due
Friday May 27
th
.