Sample Standard Operating Procedures (SOPs)

stizzahaddockSoftware and s/w Development

Dec 14, 2013 (3 years and 10 months ago)

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SAMPLE

SOP ADDRESSING LAB AND ANIMAL FACILITY BIOSAFETY PROCEDURES


Note: if you do not perform any animal work that will affect DLAR put N/A for the “DLAR Staff” SOP


Hazards:
The following materials and/or equipment associated with the use of the agent t
hat may
present exposure hazards, health hazards, and/or physical hazards.

Herpes Simplex Virus (HSV)

Attenuated and wild
-
type herpes simplex virus will be used in these experiments. Infectious virus
will only be administered intracranially. Thus, after v
irus administration, animals will be monitored
for signs of paralysis, which will denote development of encephalitis. Any animals developing
encephalitis will be immediately sacrificed following IACUC protocol. Viral shedding from the
animals which do not
develop encephalitis will be minimal. Animals which do develop encephalitis
are capable of shedding virus, but as the route of infection is intracranial, shedding upon this route
of infection is minimal. Furthermore, shedding time will be minimalized, as a
nimals will be
sacrificed upon first signs of encephalitis development.


Containment Procedures:

The following safety equipment must be used when carrying out this
procedure: (chemical fume hood, biological safety cabinet, sealed centrifuge rotors/ cover
ed
centrifuge tube buckets, filter cage top, etc.)


DLAR staff:

All work with virus
-
infected animals will be performed in the animal room following
standard animal room SOP. All infected
-
animal work will be performed in biosafety cabinet.

Lab staff:

Hand
ling or manipulating agent in lab will be performed in biosafety cabinet.
Centrifuged specimens will be loaded and removed inside the biosafety cabinet.


Protective Equipment:
The following personal protective must be worn when performing this
procedure:
(list type of glove, eye protection, lab coat, etc.)


DLAR staff:

Personnel will wear gowns, gloves, face mask, hair nets, and footies at all time while
in the animal room.

Lab staff:

Personnel will wear gowns or lab coats, gloves, at all time while in th
e lab.


Decontamination/Clean
-
Up Procedures:


DLAR staff:

Biosafety cabinet will be wiped down with Spor
-
Klenz 1:5 solution immediately
following any activity with mice. Hood will not be used for at least 20 minutes post
-
usage. All
waste (bedding, paper t
owels, etc.) associated with animals will be disposed of in biohazard bags
and autoclaved, and disposed of by DLAR procedures for the length of the stay of the animals.
Cages will be autoclaved as per DLAR protocol.


Lab staff
:

Biosafety cabinet will be w
iped down with a Spor
-
Klenz 1:5 solution, or another
appropriate disinfectant, immediately following any activity.
Hood will not be used for at least 20
minutes post
-
usage. Any small biological agent spills outside the biosafety cabinet will be covered
wit
h a paper towel and then treated with bleach (wear gloves). Let sit for at least 20 minutes and
then dispose of into autoclave bag. Spills inside the biosafety cabinet follow same procedure,
except use a non
-
bleach disinfectant.


Waste and Animal Disposal

Procedures (bagging, autoclaving, etc.):


DLAR staff:

Animal carcasses will be disposed of in marked biohazard bags and placed in
carcass freezer per standard DLAR protocols.


Lab staff:

Infectious waste, such as tubes, eppendorf tips, and other non
-
sha
rp items will be
disposed of in infectious waste containers (biohazard/ autoclave bags) within the lab. Used sharps
will be placed in a sharps container. Liquid biohazardous waste will be autoclaved, or in the
alternative, treated with bleach and dispose o
f down the sink



Injury/Exposure Response Procedures:


DLAR staff:

Any exposed area coming into contact with animals should be immediately washed
with soap and hot water (notify supervisor). Area should be observed for 7 days. Any further
treatment go to

University Health Clinic (4K). Emergencies, please contact WSU Public Safety at
577
-
2222




Lab staff:

Any exposed area coming into contact with biological agent or infected animal tissue,
should be immediately washed with soap and hot water (notify PI).

Area should be observed for 7
days. Any further treatment go to University Health Clinic (4K). Emergencies, please contact WSU
Public Safety at 577
-
2222


ADDITIONAL SPECIAL H
ANDLING PROCEDURES,
INCLUDING TRANSPORT
OF AGENTS
(SECONDARY CONTAINME
NT):


DLAR
staff:

All biosafety procedures are to be followed the entire time the mice are in the animal
room.

Lab staff:

All biosafety procedures are to be followed the entire time while in the lab. Any
transport of biological agents between labs or buildings wi
ll be done using sealed secondary
containment to prevent accidental spills.


Unattended Operations:
Describe special procedures if this experiment may be left to run
unattended:


DLAR staff:









Lab staff: