Genetic Engineering

starsbolognaBiotechnology

Dec 16, 2012 (4 years and 10 months ago)

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Genetic Engineering

What we can do with
genes

Restriction Enzymes: The Video Clip

Types of Genetic Engineering

1.
Recombinant DNA
Technology


Inserting gene from one
organism into another
(usually bacteria)


Ex: Inserting human
insulin gene into
bacterium; bacterium will
now make insulin


Recombinant DNA: You Tube
Video

Types of Genetic Engineering

2.
PCR (Polymerase Chain Reaction)


Replicating a DNA sequence over and
over and over and over again…



Allows scientists to study
specific

sequences


Applications of this include: mapping of the human genome
project, single sperm analysis, molecular archaeology and
ancient DNA, molecular ecology and behavior, disease
diagnosis and drug discovery.



Let’s See!

Gel Electrophoresis: The Video Clip

Types of Genetic Engineering

3. Gel Electrophoresis


Separates DNA into different banding patterns
based on what they contain (HOW MANY
BASES IN EACH STRAND!)


DNA separates due to opposite charge at one
end of chamber and how heavy it is


Makes a “finger
-
print”


Parents will have similar banding to their
children and vice
-
versa

Let’s See!


Benefits of Genetic Engineering

1.
Making large amounts of a human protein,
such as insulin

2.
Finding cures by altering DNA

3.
DNA Fingerprinting has helped us to see
how similar certain orgs. are

4.
Alter DNA in plants to make them resistant
to certain diseases

5.
Paternity tests!
Let’s See!