Teacher Notes - Bioinformatics Activity Bank - Franklin & Marshall ...

stalliongrapevineBiotechnology

Oct 1, 2013 (3 years and 11 months ago)

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Elizabeth Blosky







Bioinformatics Course
-
Summer 2012





Teacher Notes Template

Overview and concepts

Overview

This

lesson will allow students to become familiar with the PCR process by using the thermocycler after
discussing the process and its purpose along with completing an online simulation of it. It will also allow
the students to hypothesize which primer will w
ork best with the various prokaryotic samples and then
analyze their results after researching basic characteristics about the bacteria samples. This lesson will
occur before a
lesson where the students will test their hypothesis by completing gel electro
phoresis
and analyzing the results.

Grade level

Grades 9
-
12
(1
st

level biology course
-
college prep or honors)


Concepts covered

PCR, bacterial characteristics, primers


Prior knowledge required:

Prior to this lesson, students should have a solid understandi
ng of cell structure and function. They
should also understand how DNA is extracted from a cell (based on a pr
evious cheek cell extraction lab
),
the function of primers and the process of PCR (after completing an online simulation of the lab tool.)

Learn
ing objectives:

The following learning objectives are based on the Biology Keystone objectives:

Students will be able to
explain how genetic engineering has impacted the fields of medicine, forensics,
and agriculture (e.g., selective breeding, gene splici
ng, cloning, genetically modified organisms, gene
therapy
, gel electrophoresis, PCR
).

Activity notes

Time frame

PCR group review of purpose, technique and res
ult (5 minutes)

Discuss the 3
bacterial samples being used and the 2 primers so that students deve
lop a hypothesis for
the experiment (5 minutes)

Prepare and run PCR for 3
-
4 bacterial samples (30 minutes)

While the PCR is running, research and summarize basic characteristics about the 3
-
4 bacterial samples
and the 2 primers through PubMed (20 minutes)

Teaching Tips

This protocol is designed for a 43 minute class period. Students may need to research the bacteria and
primer information before this lab if students are not very familiar with using the research tools.


Extensions

AP level students and poss
ibly honors level biology students could potentially design primers to order
online and then run this protocol with the student
-
selected primers.


Resources

A helpful online simulation for students to practice PCR before this lab is:

http://learn.genetics.utah.edu/content/labs/pcr/

Students can access PubMed through the following website:

http://www.ncbi.nlm.nih.gov/pmc



Ackn
owledgments

These teacher notes and resources were produced after discussing research being conducted in
Dr.
Jamie Blair’s lab at Franklin and Marshall College by Jessica

Mountz.