Genetic Engineering Notesheet

1

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P a g e

Copyright 201
3

by Craig Kohn, Agricultural Sciences, Waterford WI. This
source may be freely used and distributed provided the author is cited
.

Genetic

Engineering
Notesheet

C. Kohn, Waterford WI

Name:









Hour


Date:






Date Assignment is due:





Why late?






Score:
+

✓

-




Day of Week

Date




If your project was late, describe why






What is genetic engineering
?





How is
genetic engineering

related to
the term
biotechnology
?


What are some things produced by
genetic engineering that you are
aware of?







What are some forms of biotechnology
that are not a part of genetic
engineering?

Why is genetic engineering necessary?
Why not just use traditional breeding?

Cre
ate 4 or more questions based on this topic:

1

2

3

4


Units

1. Mendelian
Genetics

2. FFA

3. DNA

4. Proteins

5. Ag Genetics

6. Biotechnology

7. Genomics

8. PCR

9. Southern
Blotting

10. Cloning

11. Stem Cells


Weekly
Schedule: See
Board and record

Mon




Tues




Wed





Thurs





Fri



2

|

P a g e

Copyright 201
3

by Craig Kohn, Agricultural Sciences, Waterford WI. This
source may be freely used and distributed provided the author is cited
.

Directions:
For

the spaces below, look ahead

through the notes. Then fill in the boxes below:


In 10
-
20 words, write a sentence
explaining
what this unit will be about.


List 4 or more terms or concepts in this unit
that you are already somewhat familiar with.


1.


2.


3.


4.

L
ist 4 or more terms or concepts in this unit
that you have never seen before or for which
have the
most limited understanding.


1.


2.


3.


4.


List 4 or more concepts or terms that you
will find more challenging than others in this
unit:


1.


2.


3.


4.


Circle one
:
I have learned some of this material in previous classes
.

Definitely


–


Yes


–


Sor琠of

-


乯

Circle one
:
I
need to review my notes & practice before the quiz.

Definitely


–


Yes


–


Sort

of

-


乯

Circle one
:
I
have never seen or heard of some of these concepts.

Definitely


–


Yes


–


Sor琠of

-


乯

Circle one
:
This may be a challenging unit for me personally.

Definitely


–


Yes


–


Sor琠of

-


乯



3

|

P a g e

Copyright 201
3

by Craig Kohn, Agricultural Sciences, Waterford WI. This
source may be freely used and distributed provided the author is cited
.

Directions
: Use the accompanying PowerPoint (
h
ttp://bit.ly/wuhs
-
genetic
-
eng
) to complete this sheet. This sheet will be
due upon the completion of the PowerPoint in class. These assignments are graded on a +/
✓
/
-

scale.


1.

When were the first genetically enginee
red crops made available to the public?





2.

What percent of the following are genetically engineered? Soybeans



Corn



Cotton



3.

Genetic engineering is














_
















4.

Define each of the following:


Recombinant DNA:














_
















Gene Knockouts:














_
















Gene Knockins:














_
















5.

One of the first uses of genetic engineering was to create









_
















6.

What is diabetes?














_
















7.

Where did diabetes

patients originally get their medicine from?









What was wrong with this?













8.

What is now used to produce insulin?












9.

What was the first step in creating this kind of E. coli?










_
















10.

Next, researchers had to













4

|

P a g e

Copyright 201
3

by Craig Kohn, Agricultural Sciences, Waterford WI. This
source may be freely used and distributed provided the author is cited
.

11.

What is a restriction enzyme?













12.

A restriction enzyme should cut DNA so that











_
















13.

What is a sticky end?














_
















14.

How can we ensure that a gene will insert itself into a host’s genome?








_
















15.

What does DNA Ligase do?













16.

Without DNA ligase, the addition of the new gene would be








17.

Summarize the four steps of creating recombinant DNA:


_
















_
















_
















_
















18.

Summarize
the 6 steps of creating Bt Corn:


_
















_
















_
















_
















_
















_
















19.

How does Bt Corn fight off pests?












_















5

|

P a g e

Copyright 201
3

by Craig Kohn, Agricultural Sciences, Waterford WI. This
source may be freely used and distributed provided the author is cited
.

20.

Is Bt Corn safe for humans?



Explain:










_
















21.

Is Bt Corn safe for the environment?



Explain:









_
















22.

What is Golden Rice?














_
















23.

What organism did scientists use to insert the genes into Golden Rice?








24.

Does Golden Rice actually make Vitamin A?



Explain:








_
















25.

Where did the genes for the
β
-
carotene
come from?










26.

What is the most common method used for producing a transgenic animal?







27.

Summarize the 5 steps of microinjection
:


_
















_
















_
















_
















_
















28.

What is a pronucleus?














29.

What are knockout mice?













_
















30.

Why would a scientist want to use a knockout mouse?










_
















6

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P a g e

Copyright 201
3

by Craig Kohn, Agricultural Sciences, Waterford WI. This
source may be freely used and distributed provided the author is cited
.

31.

Describe the four steps needed to make a knockout mouse:


_
















_
















_
















_
















32.

How do scientists inactivate a gene in a knockout mouse?









_
















33.

Knockout mice are chimeras; what does this mea
n?










_
















34.

What is a knockin mouse?













_
















35.

Why would a knockin mouse be valuable to a scientist?










_
















36.

Do you think that genetic engineering is something that researchers should use?



Explai
n:



_
















_
















_




















7

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P a g e

Copyright 201
3

by Craig Kohn, Agricultural Sciences, Waterford WI. This
source may be freely used and distributed provided the author is cited
.

Unit Wrap
-
up
C. Kohn,
Agricultural Sciences
-

Waterford WI

1.

Wri
te the 3 topics that you most need to review before the quiz:


1_












2_












3_












2.

Create 3
high
-
level
questions

related to this material

(
These questions

could be something you still don’t know or question
s that reflect

understanding that you have now that you did not have before
.
)


1_
















2_
















3_
















3.

List 6
vocabulary
words

that you did not know before or have almost never used before:


1
_





2





3






4





5





6






4.

Create a
strategy

that you have for remembering a
specific

item from this unit







_















☆
NOTE
: A strategy is
not

an activity such as reviewing your notes, making cards, studying hard, etc. A strategy is a mnemonic, rhyme, analogy, or
other brain
-
based device.

5.

Create a second different
strategy

that you have for remembering a
specific

item from this unit?



_
















6.

Create a

third different
strategy

that you have for remembering a
specific

item from this unit





_
















7.

Complete this question
after

the quiz. Circle the most appropriate response. You will only be graded on whether
or not you complet
ed this section, so be entirely honest with yourself when completing this section.


Circle one
:
I
used my notes
outside of class

to prepare for the quiz
.



Definitely
–

Yes
–

Sort of
-

No

Circle one
:
I
took
extra

notes in the margins for very difficult concepts
.


Definitely
–

Yes
–

Sort of
-

No

Circle one
:
I
created a
personal

strategy for at least two difficult items
.


Definitely
–

Yes
–

Sort of
-

No

Circle one
:
I was very involved and actively stud
ying during the quiz review
.


Definitely
–

Yes
–

Sort of
-

No

Circle one
:
I
think I will be

satisfied with the quiz grade I received this week
.


Definitely
–

Yes
–

Sort of
-

No