Human Biochemistry Laboratory, NUTR 4159 - EDU 2.0

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Oct 1, 2013 (3 years and 11 months ago)

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University of Puerto Rico

Río Piedras Campus

College of Natural Sciences


Nutrition and Dietetics Program

Program: Bachelor of Science in

Nutrition and Dietetics


Human
Biochemistry

Laboratory,
NUTR

415
9


First

Semester, 20
1
2
-
20
1
3

Academic Year

Sec. 0U1,
Wednes
day,
1
:00 p.m.


3
:50 p.m.

Room NEP
577


Michelle Schelske Santos, Ph.D.;

mschelskeuprrp@gmail.com
,

michelle.schelske@upr.edu
;

ext. 5798,
2792



Date

Assignment

Topic

22Aug12

Academic Recess


Tropical Storm Isaac



29Aug12

LAB:
Syllabus,
Preparation of LAB Notebooks

LAB
Excel workshop


Data Tables and Graphs
;

Register for course in Edu 2.0

Introduction to course

& prep of Lab Notebook

Introduction to Excel for Lab Reports
--
Water
content of foods

05Sep12

General Laboratory Safety;
E
quipment assignment
Intro to instrumentation; (bring lab coats and lab
notebooks)

Quiz in Edu2.0; open 24h before lab

General
Lab Safety;
Introduction to
Sc
ientific
Instrumentation


12Sep12

LAB Safety (Biohazard)

Post
-
conference written quiz; practical lab quiz

Micropipette and Pipette Techniques; practical lab
quiz

Blood
-
borne Pathogens and Biohazard
Disposal (OPASO; Dr. Warner Ithier)

Micropipette and
Pipetting Techniques


19Sep12

LAB


DNA Extraction

LAB


Tissue Homogenation of Protein Samples

Quiz in Edu 2.0; open 24h before lab

DNA Extraction from strawberries

Homogenation of animal & vegetable protein

26Sep
12

LAB

Computer Workshop


Info Data
bases and
your Search Strategy (Biblioteca Ciencias
Naturales; Prof. Pura Centeno)

Assign.
: Search Strategies, DNA & Protein
Method
s

Biochemical methods for DNA
and protein
analys
e
s in the field of nutrition


03Oct12

LAB



Total Protein Determination

Quiz

in Edu 2.0; open 24h before lab


Due: Assignment Search Strategies DNA &
Protein Method

Protein content of animal versus vegetable
protein on microscale using microplate reader;

Comparison of protein content of human milk,
cow milk and infant formula

10Oct12


LAB Expt: Active Acidity and pH
;


Quiz in
Edu2.0

open 24h before lab


Active Acidity and pH

Dra
. M. Schelske
Santos; NUTR 4159


sem.
1
, 201
2
-
201
3



Page
2

of
2

17Oct12


LAB:

Protein Electrophoresis of blood fractions I

Quiz in Edu 2.0; open 24h before lab

Preparation of blood fractions

Preparation for and dry run

of electrophoresis

24Oct12

LAB:

Protein Electrophoresis of blood fractions II

Quiz in Edu 2.0; open 24h before lab


Protein electrophoresis, gel staining & drying

31Oct12

LAB


Expt. Membrane Structure and Function;
Quiz in Blackboard; open 24h before
lab

Blood Typing

Digital Microscopy of Blood Cells

Fri., 02Nov12

LAB OP1


(50 pts.
; 1h
)

Bioinformatics Assignment

Experiments to date, including
theory and
procedure of SDS_PAGE (not graph & ptn ID)

Use of experimental and stored data

07Nov12


Class
instead of lab


14Nov12

LAB

Glycolytic fermentation;
Fiber
and Lipids

Quiz in Edu 2.0; open 24h before lab


Due SDS
-
PAGE Lab (in notebook)

Due Bioinformatics (Edu 2.0)

Anaerobic fermentation of sugars by yeast

Dietary Lipids, Fiber & Fattaché

21Nov12

Libre


Happy Thanksgiving


28Nov12

Extraction of DNA from whole blood (TBconfirmed)


Extraction of DNA from student leucocytes

from venupuncture blood draw by nurse from
Medical Services

05Dec12

Electrophoresis of DNA

Horizontal agarose gel
electrophoresis of
nucleic acids

12Dec12

(
Rep.22aug12
)

Oral Presentations (7 students)

Lab Conclusions

Due: Written
reports


TBA, Dec.
17
-
21, 2012

Lab OP 2



(50 pts.
; 1h
)

Due: Lab Notebooks

(Experiments from second half of semester)


XXXXXX

LAB: Expt.

30, Vitamin C

Quiz in Edu 2.0; open 24h before lab



Colorimetric Determination of Vitamin C: Raw
vs. Processed Foods


This schedule is subject to change based on unforeseen events,
natural disasters, scheduling of
events
,
and
changes in

laboratory equipment and material

arrival dates. In the event of any change, a new schedule will be
developed and available in
Edu 2.0
.




Edited by MSS,
01nov
12