Teacher Notes - Bioinformatics Activity Bank

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Oct 1, 2013 (3 years and 10 months ago)

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Introduction to ABO Blood Typing


Overview and concepts

Overview

This lesson is an introductory activity on blood types. It emphasizes the use of Punnett

Squares for
predicted blood types, as well as the introduction to the antigens and antibodies involved in
determining blood type. In addition to Punnett Squares the class will look at blood type on a molecular
level by analyzing the nucleotides involved
in the gene for blood type as well as sample Light Clycer Data
from the Bioinformatics workshop at F &M.


Grade level

9
-
12


Concepts covered

Genetics, Punnett Squares, antigens, antibodies, blood type, assays, Single Nucleotide Polymorphisms
(SNPs)


Prior
knowledge required
:

Dominance, recessive, Punnett Squares, alleles, genes, nucleotides, SNPs


Activity notes

Time frame



Total Time: 90


130 minutes



Pre
-
Assessment: 10 min



Blood Type Punnett Square Practice : 10
-
15 min



“Whodunnit? Blood Type Lab” Prelab

& Scenario: 45 min



Blood Type Light Cycler Data (demonstration, then small group discussion): 15
-
20 min



Analysis of nucleotides of Blood Type Gene (as a class or in small groups) 15
-
20 min



Blood Typing Worksheet 20 min



Post
-
Assessment 20
-
25 min


Materials

Flinn Scientific: Simulated ABO/Rh Blood Typing Kit

Attached Worksheets

Student Computers for Extension Portion

Projector to analyze Light Cycler Data and Nucleotide Sequence.


Teaching Tips



Depending on your level of students, it might be helpf
ul to do a review of blood typing prior
to beginning the lesson



Depending on the materials available, the students will need to be in groups.



Separate the solutions from the Ward’s activity into additional bottles to create more
solutions
for the other gro
ups



Run a test for the students to see what agglutination looks like prior to the lab.



Instructional Sequence:

o

Warm
-
Up: Pre
-
Assessment

o

“Whodunnit: Blood Typing Lab” Prelab

o

Review antigens, antibodies, and agglutination as it applies to blood typing

o

“Whodun
nit: Blood Typing Lab” Using Flinn Scientific’s Simulated ABO/Rh Blood
Typing Kit

o

Give each group a copy of the “Light Cycler Sample Data” and the “ABO Nucleotide
Sequence”.

o

Review how to analyze Light Cycler Data and Nucleotide Seuqence Data with the
cl
ass. Do a few sample problems together or in small groups.

o

Have students complete the “Extension” portion of the lab in their small groups.

o

Have students complete the “ABO Blood Type Worksheet”

o

Post
-
Assessment


Assessment

Pre
-
assessment: short answer quiz
. Post
-
assessment: analysis of the student's individual blood type and
molecular genotype.


Extensions



Blood Typing Game and Reading
http://nobelprize.org/educational/medicin
e/landsteiner/index.html



Perform actual student Blood Typing with the assistance of the school nurse and the Blood
Typing kit from Ward’s


Resources



American Red Cross: Students can research about the different blood types, donor and
recipients, and approximate percentages across the population.
http://www.redcrossblood.org/lear
n
-
about
-
blood/blood
-
types



The Biology Project: Blood Types Tutorial. Additional information about ABO markers,
genotypes, and the Rh factor, as well as student problem sets.
http://www.biology.arizona.edu/human_bio/problem_sets/blood_types/intro.html


Ackn
owledgments

The teacher notes were produced by Beth Sholder. The
“Whodunnit? Blood Type Lab” is a modification
of a Ward’s activity. The blood type nucleotide analysis and the Light Cycler data was obtained from the
Bioinformatics Seminar at F & M.