Introduction to ABO Blood Typing
Overview and concepts
This lesson is an introductory activity on blood types. It emphasizes the use of Punnett
predicted blood types, as well as the introduction to the antigens and antibodies involved in
determining blood type. In addition to Punnett Squares the class will look at blood type on a molecular
level by analyzing the nucleotides involved
in the gene for blood type as well as sample Light Clycer Data
from the Bioinformatics workshop at F &M.
Genetics, Punnett Squares, antigens, antibodies, blood type, assays, Single Nucleotide Polymorphisms
Dominance, recessive, Punnett Squares, alleles, genes, nucleotides, SNPs
Total Time: 90
Assessment: 10 min
Blood Type Punnett Square Practice : 10
“Whodunnit? Blood Type Lab” Prelab
& Scenario: 45 min
Blood Type Light Cycler Data (demonstration, then small group discussion): 15
Analysis of nucleotides of Blood Type Gene (as a class or in small groups) 15
Blood Typing Worksheet 20 min
Flinn Scientific: Simulated ABO/Rh Blood Typing Kit
Student Computers for Extension Portion
Projector to analyze Light Cycler Data and Nucleotide Sequence.
Depending on your level of students, it might be helpf
ul to do a review of blood typing prior
to beginning the lesson
Depending on the materials available, the students will need to be in groups.
Separate the solutions from the Ward’s activity into additional bottles to create more
for the other gro
Run a test for the students to see what agglutination looks like prior to the lab.
“Whodunnit: Blood Typing Lab” Prelab
Review antigens, antibodies, and agglutination as it applies to blood typing
nit: Blood Typing Lab” Using Flinn Scientific’s Simulated ABO/Rh Blood
Give each group a copy of the “Light Cycler Sample Data” and the “ABO Nucleotide
Review how to analyze Light Cycler Data and Nucleotide Seuqence Data with the
ass. Do a few sample problems together or in small groups.
Have students complete the “Extension” portion of the lab in their small groups.
Have students complete the “ABO Blood Type Worksheet”
assessment: short answer quiz
assessment: analysis of the student's individual blood type and
Blood Typing Game and Reading
Perform actual student Blood Typing with the assistance of the school nurse and the Blood
Typing kit from Ward’s
American Red Cross: Students can research about the different blood types, donor and
recipients, and approximate percentages across the population.
The Biology Project: Blood Types Tutorial. Additional information about ABO markers,
genotypes, and the Rh factor, as well as student problem sets.
The teacher notes were produced by Beth Sholder. The
“Whodunnit? Blood Type Lab” is a modification
of a Ward’s activity. The blood type nucleotide analysis and the Light Cycler data was obtained from the
Bioinformatics Seminar at F & M.