study guide to Ch 15 - Restriction Enzymes, DNA Fingerprinting


Dec 12, 2012 (4 years and 7 months ago)


Ch. 15 Study Guide

Restriction Enzymes

Used to cut DNA into smaller fragments

Leaves the ends of fragments with STICKY ENDS (see picture


These STICKY ENDS are where inserted DNA fragments can attach

The complimentary bases (ACTG) will attach


Bacteria can incorporate genes from other organisms and use them to produce the
corresponding proteins. This is called transformation.




maintain desirable


create animal with
desirable traits from 2
different animals

Induced Mutations

Use of radiation or
chemicals to create
new genetic variations

Plasmids are rings of DNA in bacteria that can be used to transform bacteria. This is why these
are widely

used in genetic engineering. (see picture)

Clones are organisms that are produced by taking a body cell and placing the nucleus into an
nucleated egg. The resulting offspring are genetically identical to the adult organism from
which the body cel
l was taken. (Dolly) (see picture)

GM (genetically modified)

Genetic engineering (changing of the DNA sequence of ATCGs) can create genetically
modified organisms. For GM crops there can be many advantages (faster growth, resistance
to pests, great
er yields etc.)

Gene Therapy

This process uses genetically modified viruses which are
inserted into humans to help
correct some medical disorder or disease. (see picture)

DNA Fingerprinting

Process of analyzing DNA fragments from gel
electrophoresis to compare samples of
DNA. This is valuable because no two people other than identical twins have the same
DNA.(see picture)

2008 Congress passed the
Genetic Information
Act. This law makes it illegal
to use a
person’s genetic information to discriminate against them.