SG Genetic Eng and Biotech test.docx - Jessica Sullivan

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Dec 12, 2012 (4 years and 11 months ago)

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Study Guide: Genetic Engineering and Biotechnology (all of topic 4.4)

Before you get started:



Read through topic 4.4 in the IB Bio Syllabus



Go to the Topic Review on Click4Biology (
http://click4biology.info/c4b/4/gene4.4.htm#one
)
There is
also
a link to this site under Monday’
s homework on the wiki.



Use the Topic Review on Click4Biology to guide
and support
your studying.

The test will consist of 14 multiple choice questions, 3 shor
t answer questions and one data based
question.

The following open ended questions cover the material that will be on the test. Answer these questions
as completely and thoroughly as possible.

1.

What are restriction enzymes used for? List at least two proc
esses.

2.

What does PCR stand for and what does it do?

3.

What is recombinant DNA?

4.

How are plasmids used to make recombinant DNA?

5.

Describe the technique to transfer a gene of interest into a plasmid.

6.

What is the process of gel electrophoresis? What is it used

for?

Give an example

7.

Why are genes able to be transferred between different living organisms and still code for the
same amino acids?

8.

What is the role of DNA ligase in recombinant DNA technology?

9.

What are concerns about having genetically modified organi
sms that are released into the wild?

10.

Draw a gel
with the banding patterns of 4 samples of DNA. What does it mean if two of the
banding patterns match? What are some examples where gel electrophoresis can be used?

11.

What are GMOs? What are the ethical is
sues surrounding GMOs (both for and against)?