Conclusion - Weston Brand Biotech

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Dec 12, 2012 (4 years and 8 months ago)

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Project Lead The Way, Inc.

Copyright 2010

PLTW
TM

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BE


Unit 3


Activity 3.1.6


Genetic Engineering: Making E.Coli Glow Like Jellyfish


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1

Conclusion


1.

What are the ethical considerations involved in genetic engineering?

The ethical considerations involved in genetic engineering are whether or not we should
really be using this on humans and animals to change their genetic make up



2.

What are
some beneficial applications of recombinant DNA technology?

A benefit would be to use this as a way to give someone a resistent to certain types of
illness.



3.

Why is
E. coli

HB 101 a good candidate for a bioreactor?

It has a high yield production of recom
binant protein.



4.

What genes are found on the pGlo plasmid and what are there functions?

AmpR, araC and gfp or bla




5.

How does a genetic engineer distinguish bacteria containing the plasmid DNA
from those bacteria lacking the plasmid DNA?

If the bacteria c
ontains plasmid dna, and chromosomal dna. Plasmid DNA is not natural
to the bacteria and is introduced through conjugation or done by recombinant DNA




6.

How does a genetic engineer determine if the bacterium has truly been
transformed?

They check for the
gene expression



7.

How does heat shock assist in gene insertion?

It helps to open the plasma membrane of the bacteria and allow the plasmid into the cell




8.

How does electroporation assist in gene insertion?

This process increases the permeability of the ce
ll plasma membrane