Protein concentrations at equilibrium as a function of the square of the centrifuge cell radius, using assumed molecular masses of a single sedimenting species, were calculated using the Ultrascan Analysis software package (Demeler, 2005) for each of three protein concentrations and three centrifgutaion speeds. Variance and standard deviations between the calculated- and measured protein concentration at each position in the cell were determined using the software. "Quality" indicates the overall fit between the calculated protein distribution for each model and the concentrations measured in the cell.

rapidcrimsonMechanics

Feb 22, 2014 (3 years and 5 months ago)

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Supplemental Table S1.

Curve Fitting to
Calculated

Sedimentation Equilibrium Distributions

Parameter

Predictions based on 85 kDa monomer


Optimal prediction: single
component, 157.9 kDa


Monomer

Dimer

Trimer

Tetramer



Variance

6.30 x 10
-
4

3.93 x 10
-
5

8.03 x 10
-
4

2.22 x 10
-
3


2.27 x 10
-
5

St
.

Dev
.

2.51 x 10
-
2

6.27 x 10
-
3

2.83 x 10
-
2

4.71 x 10
-
2


4.76 x 10
-
3

Quality

Poor

Good

Poor

Poor


Good

Protein concentrations
at equilibrium
as a function of the square of the centrifuge cell radius
,

using assumed molecular masses of a single sedimenting species
,

were calculated using the
Ultrascan Analysis software
package
(Demeler, 2005)

for each of three protein concentrations
and three centrifgutaion speeds. Variance and standard deviations between the calculated
-

and

measured protein concentration at each position in the cell were determined using the software.
"Q
uality" indicates the overall fit between the calculated protein distribution for each model and
the concentrations measured in the cell.