Olympus FV1000 Laser Lines, Objective and Other Information

11/6/2013


1


Olympus FV1000 Laser Lines, Objective and Other Information

Microscope
"Name"
:

Olympus Fluoview 1000 inverted microscope
with IX81.

Acquisition Software:


Olympus FV1000 2.0c


A
nalysis

Software:


FV10
-
ASW
2.0

Viewer


Laser Lines:

405


LD

458


Multi
-
Argon

488


Multi
-
Argon

515


Multi
-
Argon

561


Solid State

633


HeNe

Objectives:

Name


Power


NA

UPLSAPO


4x



0.16

UPLSAPO


10x



0.4


DIC 3

Name


Power


NA

UPLFLN


10x 2PH


0.30

PH 1

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UPLFLN


40x

Oil


1.3


DIC 2

PLAPON


60x

Oil


1.42

DIC 4

LUCPLFLN


40x



0.60

Cover slip Correction


Not in microscope at this time:

UPLSAPO


20x



0.75 DIC 5


Scan Speed:

2.0 µs/pixel
-

200.0 µs/pixel
-

this can vary and may
not be what you scanned your
images at.

50x
-

Optical Zoom

-

this is the maximum capability of the microscope


Other analysis programs that may have been used:

ImageJ

1.4
5H

-

Wayne Rasband National Institutes of Health


MIPAV version
5
.
3
.2
-

Medical Image Processing, Analysis and
Vis
ualization. Center for Information Technology [CIT] National
Institutes of Health [NIH].


Any publications which use MIPAV to quantify or



visualize data are required to reference MIPAV by:



McAuliffe MJ, Lalonde FM, McGarry D, Gandler W,

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Csaky K, Trus BL. Medical Image Processing, Analysis &


Visualization In Clinical Research. IEEE COMPUTER
-
BASED


MEDICAL SYSTEMS (CBMS) 2001, 381
-
386.


Imaris

x64
7
.
2
.0 [Nov 27 2007] Bitplane AG

Zeiss

LSM Image Browser Version 4.2.0.121
-

CarlZeiss MicroImaging

Zen 2009 Light Edition


Zeiss NLO5
1
0

confocal laser scanning microscope

: Carl Zeiss, Jena,
Germany.


Lasers:


Argon/2


458,477,488,514nm





HeNe1


543nm





HeNe2


633nm





Titanium Sapphire

780nm



Objectives:

Plan
-
Neofluar

10x/0.3 NA

DIC Plan Neofluar





Plan
-
Neofluar

20x/0.5 NA






Plan
-
Neofluar

40x/1.3 NA Oil DIC

III





Acroplan


63x/0.95 NA Water





Plan
-
Neofluar

100x/1.3 NA Oil

DIC

III

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C
-
Apochrom LD 40x/1.1 W Kor

UV
-
Vis
-
IR DIC III


Zeiss UV510

confocal laser scanning microscope

: Carl Zeiss, Jena,
Germany.


Lasers:


Argon/2


458,477,488,514nm





DPSS


561
-
1

561nm





HeNe594


594nm





HeNe633


633nm





Diode 405
-
30

405nm


Objectives:

CP Achromat 5x/0.12 NA





Plan
-
Apochromat 10x/0.45 NA





Achrostimat 20x/0.3

PH I









Plan
-
Apochromat 20x/0.8NA





Plan
-
Neofluor 40x/1.3 Oil DIC

III





Plan
-
Apochromat 63x/1.40.1 DIC M27

DIC III





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Zeiss LCI510 confocal laser scanning microscope

: Carl Zeiss, Jena,
Germany.


Lasers:


Argon/2


458,477,488,514nm





DPSS


561
-
10

561nm





HeNe594


594nm





HeNe633


633nm





Diode 405
-
30

405nm


Objectives:

Plan
-
Apochromat 10x/0.45 NA

DIC II





Plan
-
Apochromat 20x/0.8NA





Plan
-
Neofluor

40x/1.3 Oil DIC

DIC III





Plan
-
Apochromat 63x/1.40.1 DIC M27

DIC III

Extra Zeiss Objectives [not in any microscope]

C
-
Aplochromal

63x/1.2 NA W Corr. DIC II







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Zeiss LSM710 confocal laser scanning microscope

: Carl Zeiss, Jena,
Germany.


Lasers:


Argon


458, 488, 514





DPSS
561
-
10


561





HeNe594



594





Chameleon


690
-
1064





HeNe 633



633





Diode 405
-
30


405


Objectives:

Pln Apo 10x
/0.45NA

DICII, Brightfield





C Apo 10x/0.45NA W Brightfield





Pln Apo 63x/1.4NA

DICII, Brightfield





LD LCI Plan
-
Apo Koor

25x/0.8NA


DICII, Brightfield









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TIRF and Super
-
resolution imaging in B538




Live cell TIRF imaging




Super
-
resolution PALM imaging: fixed cells labeled with
photoactivatible fluorescent proteins




Super
-
resolution SIM imaging in conjunction with NIH i
maging
lab: fixed cells with any normal fluorescent dyes




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