11/6/2013
1
Olympus FV1000 Laser Lines, Objective and Other Information
Microscope
"Name"
:
Olympus Fluoview 1000 inverted microscope
with IX81.
Acquisition Software:
Olympus FV1000 2.0c
A
nalysis
Software:
FV10
-
ASW
2.0
Viewer
Laser Lines:
405
LD
458
Multi
-
Argon
488
Multi
-
Argon
515
Multi
-
Argon
561
Solid State
633
HeNe
Objectives:
Name
Power
NA
UPLSAPO
4x
0.16
UPLSAPO
10x
0.4
DIC 3
Name
Power
NA
UPLFLN
10x 2PH
0.30
PH 1
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UPLFLN
40x
Oil
1.3
DIC 2
PLAPON
60x
Oil
1.42
DIC 4
LUCPLFLN
40x
0.60
Cover slip Correction
Not in microscope at this time:
UPLSAPO
20x
0.75 DIC 5
Scan Speed:
2.0 µs/pixel
-
200.0 µs/pixel
-
this can vary and may
not be what you scanned your
images at.
50x
-
Optical Zoom
-
this is the maximum capability of the microscope
Other analysis programs that may have been used:
ImageJ
1.4
5H
-
Wayne Rasband National Institutes of Health
MIPAV version
5
.
3
.2
-
Medical Image Processing, Analysis and
Vis
ualization. Center for Information Technology [CIT] National
Institutes of Health [NIH].
Any publications which use MIPAV to quantify or
visualize data are required to reference MIPAV by:
McAuliffe MJ, Lalonde FM, McGarry D, Gandler W,
11/6/2013
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Csaky K, Trus BL. Medical Image Processing, Analysis &
Visualization In Clinical Research. IEEE COMPUTER
-
BASED
MEDICAL SYSTEMS (CBMS) 2001, 381
-
386.
Imaris
x64
7
.
2
.0 [Nov 27 2007] Bitplane AG
Zeiss
LSM Image Browser Version 4.2.0.121
-
CarlZeiss MicroImaging
Zen 2009 Light Edition
Zeiss NLO5
1
0
confocal laser scanning microscope
: Carl Zeiss, Jena,
Germany.
Lasers:
Argon/2
458,477,488,514nm
HeNe1
543nm
HeNe2
633nm
Titanium Sapphire
780nm
Objectives:
Plan
-
Neofluar
10x/0.3 NA
DIC Plan Neofluar
Plan
-
Neofluar
20x/0.5 NA
Plan
-
Neofluar
40x/1.3 NA Oil DIC
III
Acroplan
63x/0.95 NA Water
Plan
-
Neofluar
100x/1.3 NA Oil
DIC
III
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C
-
Apochrom LD 40x/1.1 W Kor
UV
-
Vis
-
IR DIC III
Zeiss UV510
confocal laser scanning microscope
: Carl Zeiss, Jena,
Germany.
Lasers:
Argon/2
458,477,488,514nm
DPSS
561
-
1
561nm
HeNe594
594nm
HeNe633
633nm
Diode 405
-
30
405nm
Objectives:
CP Achromat 5x/0.12 NA
Plan
-
Apochromat 10x/0.45 NA
Achrostimat 20x/0.3
PH I
Plan
-
Apochromat 20x/0.8NA
Plan
-
Neofluor 40x/1.3 Oil DIC
III
Plan
-
Apochromat 63x/1.40.1 DIC M27
DIC III
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5
Zeiss LCI510 confocal laser scanning microscope
: Carl Zeiss, Jena,
Germany.
Lasers:
Argon/2
458,477,488,514nm
DPSS
561
-
10
561nm
HeNe594
594nm
HeNe633
633nm
Diode 405
-
30
405nm
Objectives:
Plan
-
Apochromat 10x/0.45 NA
DIC II
Plan
-
Apochromat 20x/0.8NA
Plan
-
Neofluor
40x/1.3 Oil DIC
DIC III
Plan
-
Apochromat 63x/1.40.1 DIC M27
DIC III
Extra Zeiss Objectives [not in any microscope]
C
-
Aplochromal
63x/1.2 NA W Corr. DIC II
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Zeiss LSM710 confocal laser scanning microscope
: Carl Zeiss, Jena,
Germany.
Lasers:
Argon
458, 488, 514
DPSS
561
-
10
561
HeNe594
594
Chameleon
690
-
1064
HeNe 633
633
Diode 405
-
30
405
Objectives:
Pln Apo 10x
/0.45NA
DICII, Brightfield
C Apo 10x/0.45NA W Brightfield
Pln Apo 63x/1.4NA
DICII, Brightfield
LD LCI Plan
-
Apo Koor
25x/0.8NA
DICII, Brightfield
11/6/2013
7
TIRF and Super
-
resolution imaging in B538
Live cell TIRF imaging
Super
-
resolution PALM imaging: fixed cells labeled with
photoactivatible fluorescent proteins
Super
-
resolution SIM imaging in conjunction with NIH i
maging
lab: fixed cells with any normal fluorescent dyes
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