GENOME ENGINEERING : RESEARCH & APPLICATIONS

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Dec 10, 2012 (4 years and 6 months ago)

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G
ENOME
E
NGINEERING
:


R
ESEARCH
&

A
PPLICATIONS

S
EPTEMBER
2-7,

2012
L
UCCA
,

I
TALY


O
RGANIZERS
:
Toni Cathomen
J. Keith Joung
Theirry Vandendriessche












T
HE
F
EDERATION GRATEFULLY ACKNOWLEDGES THE FOLLOWING
EDUCATIONAL GRANTS FOR THE

FASEB

S
CIENCE
R
ESEARCH
C
ONFERENCES
:

Platinum Level Support:

Sigma-Aldrich

Gold Level Support:



Sangamo Biosciences, Inc.
Silver Level Support:

The Jackson Laboratory
Bronze Level Support:

New England Biolabs, Inc.




Genome Engineering – Research & Applications
September 2–7, 2012
Lucca, Italy


Co-Organizers:
Toni Cathomen
Universitätsklinikum Freiburg
Freiburg, Germany

J. Keith Joung
Massachusetts General Hospital
Boston, MA, U.S.A.

Thierry Vandendriessche
Vrije Universiteit Brussel
Brussels, Belgium





Sunday,
September 2, 2012


4:00 PM – 9:00 PM Conference Registration

6:00 PM – 7:00 PM FASEB Opening Reception

7:00 PM – 8:30 PM Dinner

8:30 PM – 8:45 PM Opening remarks

8:45 PM – 10:00 PM
Keynote Lecture
Maria Jasin, Memorial Sloan-Kettering Cancer Center
Genome modification and DNA repair.
Monday,
September 3, 2012


7:30 AM – 9:00 AM Breakfast

9:00 AM – 9:15 AM Welcome from FASEB

Session 1:
9:15 AM – 12:15 PM

DNA repair – the foundation of genome engineering
Session Chair: Nancy Maizels, University of Washington, Seattle

9:15 AM – 9:45 AM Matthew Weitzman, The Children’s Hospital of Philadelphia
Genomes in Conflict: Cellular DNA Repair During Virus Infection

9:45 AM –10:15 AM Lee Zou, Massachusetts General Hospital, Charlestown
Sensing of DNA breaks by the ATM and ATR checkpoint kinases

10:15 AM – 10:45 AM Group photo & FASEB Sponsored Coffee Break


10:45 AM – 11:15 AM Nancy Maizels, University of Washington, Seattle
Using pathways optimized by evolution to engineer the genome:
targeted gene correction at DNA nicks.

11:15 AM – 11:45 PM David B. Roth, University of Pennsylvania
Nuclease stimulated gene modification: assessing and minimizing
off target effects

Session 2:
11:45 AM – 10:30 PM

Engineering customized nucleases (I)
Session Chair: Philippe Duchateau, Cellectis S.A., Paris

11:45 AM – 12:15 PM J. Keith Joung, Massachusetts General Hospital, Charlestown
FLASH assembly of TALENs enables high-throughput genome
editing.

12:15 PM – 1:15 PM Lunch & Meet-the-Investigator (Keynote Speaker and speakers
from Session 1)

Free Afternoon

5:00 PM – 7:00 PM
Poster Session 1

7:30 PM – 8:30 PM Dinner

8:30 PM – 9:00 PM Julianne Smith, Cellectis Therapeutics, Paris
Designing nucleases for human gene therapy.

9:00 PM – 9:30 PM Thomas Lahaye, Ludwig-Maximilians University, Munich
TAL effector targets and how to find them.

9:30 PM – 9:45 PM Jeffrey C. Miller, Sangamo BioSciences, Inc., Richmond
Beyond the TALE code: new RVDs and context-dependent
strategies for creating improved TALENs.

9:45 PM – 10:00 PM Benjamin P. Kleinstiver, Western Ontario University, London
Monomeric nucleases for genome engineering applications.

10:00 PM – 10:30 PM Adam Bogdanove, Cornell University, Ithaca
Code refinement and computational tools for better TAL effector
design and target prediction.
Tuesday,
September 4, 2012


7:30 AM – 9:00 AM Breakfast


Session 3
9:00 AM – 12:15 PM

Engineering customized nucleases (II)
Session Chair: Daniel Voytas, University of Minnesota

9:00 AM - 9:30 AM Srinivasan Chandrasegaran, Johns Hopkins University
Zinc finger nucleases (ZFNs) – a historic perspective.

9:30 PM –10:00 AM Edward J. Rebar, Sangamo BioSciences
Development of targeted nucleases for genome engineering.

10:00 AM – 10:15 AM Sandrine J. Boissel, University of Washington, Seattle
MegaTALs: a novel designer rare-cleaving nuclease scaffold for
highly active and specific genome engineering.

10:15 AM – 10:45 AM FASEB Sponsored Coffee Break


10:45 AM – 11:00 AM Gregory D. Davis, Sigma-Aldrich Biotechnology, St. Louis
Targeted genome editing using zinc finger nucleases with ssDNA
oligos and alternative donor formats.

11:00 AM – 11:30 AM Alfred Pingoud, University of Giessen
TALE-PvuII fusion proteins – novel tools for gene targeting.

11:30 AM – 12:00 PM Scot Wolfe, University of Massachusetts Medical School
Expanding the flexibility of zinc finger nucleases for targeted
genome editing.

12:15 PM – 1:15 PM Lunch & Meet-the-Investigator (Speakers from Sessions 2 & 3)

Free Afternoon: Optional Trip to Barga

7:30 PM – 8:30 PM Dinner

Session 4:
8:30 PM – 10:30 PM

Genome engineering for synthetic biology
Session Chair: Pernette Verschure, University of Amsterdam

8:30 PM – 8:45 PM Jeffry D. Sander, Massachusetts General Hospital, Charlestown
Oligomerized pool engineering (OPEN) 2.0: A simplified zinc finger
nuclease engineering platform with expanded targeting range.

8:45 PM – 9:00 PM Jaehwan Jeong, Yonsei University
Genome-scale promoter engineering by coselection MAGE.

9:00 PM – 9:30 PM Thierry Vandendriessche, Free University of Brussels
Synthetic biology meets gene therapy: doing better than nature?

9:30 PM – 10:00 PM Martin Fussenegger, Swiss Federal Institute of Technology
Engineering mammalian cells for therapeutic applications.

10:00 PM – 10:30 PM Pernette Verschure, University of Amsterdam
Design principles of epigenetic gene regulation in higher
eukaryotes: Systems Biology approaches using mammalian
synthetic cell systems.
Wednesday,
September 5, 2012


7:30 AM – 9:00 AM Breakfast

Session 5
9:00 AM – 10:30 PM

Transposable elements for genome modification
Session Chair: Nancy L. Craig, Johns Hopkins University

9:00 AM – 9:30 AM Zoltan Ivics, Paul-Ehrlich-Institute, Langen
Retargeting Sleeping Beauty transposons and targeted gene
insertion in transposon-tagged loci in iPSCs and transgenic pigs.

09:30 AM – 10:00 AM Eric Ostertag, Transposagen
XTN TALEN and piggyBac technology for the creation of genetically
modified rat models.

10:00 AM – 10:30 AM Nancy L. Craig, Johns Hopkins University
piggyBac transposition.

10:30 AM – 11:00 AM FASEB Sponsored Coffee Break


Session 6
11:00 AM – 12:00 PM

Cutting edge topics in genome engineering
Session Chair: Toni Cathomen, University Medical Center Freiburg

11:00 AM – 11:15 AM Scott C. Fahrenkrug, Recombinetics, St. Paul,
Gene inactivation and non-meiotic allele introgression in livestock
species using TALENs.

11:15 AM – 11:30 AM Charles A. Gersbach, Duke University
Genetic correction of Duchenne Muscular Dystrophy by engineered
nucleases.

11:30 AM – 11:45 AM Tobias Schmidt, University of Bonn
Rapid generation of ZFN- or TALEN-mediated knock out cell lines
employing a deep sequencing screening technology.

11:45 AM – 12:00 PM Takahito Watanabe, University of Tokushima
Efficient production of knockout crickets using custom designed
nucleases, ZFNs and TALENs.

12:15 PM – 1:15 PM Lunch & Meet-the-Investigator (Speakers from Sessions 4–6)

Free Afternoon

5:00 PM – 7:00 PM
Poster Session 2


7:30 PM – 8:30 PM Dinner


Session 7:
8:30 PM – 10:30 PM

Targeted genome modification with recombinases and
integrases
Session Chair: Anja Ehrhardt, University of Witten/Herdecke

8:30 PM – 8:45 PM Yanru Chen-Tsai, Stanford University
Site-specific gene modification in transgenic mouse models and cell
lines mediated by integrase.

8:45 PM – 9:00 PM Ben Davies, Wellcome Trust Centre for Human Genetics
New recombinases for genome engineering.


9:00 PM – 9:30 PM Anja Ehrhardt, University of Witten/Herdecke
Molecular design and efficacy of viral hybrid-vectors for efficient
delivery of recombinases in vitro and in vivo.


9:30 PM – 10:00 PM Carlos F. Barbas III, The Scripps Research Institute, La Jolla
Design and evolution of zinc finger enzymes: Novel nucleases and
recombinases for genome editing.

Thursday,
September 6, 2012


7:30 AM – 9:00 AM Breakfast


Session 8
9:00 AM – 12:15 PM

Genome engineering in model organisms
Session Chair: Dana Carroll, University of Utah

9:00 AM – 9:30 AM Daniel Voytas, University of Minnesota, Minneapolis

Targeted modification of plant genomes.

9:30 AM – 10:00 AM Joanna Yeh, Massachusetts General Hospital, Charlestown
High success rates and mutation efficiencies of TALENs in
zebrafish.

10:00 AM – 10:15 AM Dietmar J. Kappes, Fox Chase Cancer Center
Functional dissection of transcriptional control elements at the
ThPOK locus using ZFNs and TALENs.

10:15 AM – 10:45 AM FASEB Sponsored Coffee Break

10:45 AM – 11:00 AM Raman Sood, National Human Genome Research Institute
Targeted mutagenesis of nine genes in zebrafish: General
considerations with commercial and public sources of ZFNs.

11:00 AM – 11:30 AM Ignacio Anegon, INSERM UMR 1064, Nantes
Applying ZFNs, TALENs and meganucleases to generate knockout
and knockin rats.

11:30 AM – 12:00 PM Dana Carroll, University of Utah School of Medicine, Salt Lake City
Gene targeting in Drosophila with ZFNs and TALENs.

12:00 PM – 12:30 PM
Business Meeting

12:45 PM – 1:45 PM Lunch & Meet-the-Investigator (Speakers from Sessions 7 & 8)

Free Afternoon: Optional Trip to Lucca

6:30 PM – 8:30 PM Dinner & Meet-the-Investigator (Speakers from Sessions 9 & 10)

Session 9:
8:30 PM – 10:00 PM

Top Young Investigator Abstracts
Session Chair: J. Keith Joung, Massachusetts General Hospital

8:30 PM – 8:45 PM Ines Fonfara, Umeå University, Sweden
Programmable dual-RNA-guided site-specific DNA endonucleases.

8:45 PM – 9:00 PM Claudio Mussolino, University Medical Center Freiburg
Genome surgery using designer nucleases: ZFNs or TALENs?

9:00 PM – 9:15 PM Fayza Daboussi, Cellectis S.A., Paris
Approaches to improve gene disruption using endo/exonuclease
co-expression.

9:15 PM – 9:30 PM Jonathan L. Schmid-Burgk, University of Bonn
Generation of TALE genes at high-throughput using a ligation-
independent assembly approach.

9:30 PM – 9:45 PM Morgan L Maeder, Massachusetts General Hospital, Charlestown
Synergistic activation of gene expression by FLASH-assembled
TALE transcription factors.

Friday,
September 7, 2012


7:30 AM – 8:30 AM Breakfast


Session 10
8:30 AM – 11:30 PM

Genome engineering for therapeutic applications
Thierry Vandendriessche, Free University of Brussels

8:30 AM – 9:00 AM Luigi Naldini, San Raffaele Telethon Institute for Gene Therapy
Sustainable gene transfer using zinc-finger nucleases.



9:00 AM – 9:30 AM Paula Cannon, University of Southern California
Genetic modification of human hematopoietic stem cells using zinc-
finger nucleases.

9:30 AM – 10:00 AM Toni Cathomen, University Medical Center Freiburg
Targeted gene correction in a mouse disease model.

10:00 AM – 10:15 AM Break

10:15 AM – 10:45 AM Andrew Scharenberg, Cellectis Therapeutics
T-cell engineering using TAL-effector nuclease (TALEN) mRNA
electroporation.

10:45 AM – 11:15 AM Philip Gregory, Sangamo BioSciences Inc.
Blocking HIV infection with ZFNs – an update from the clinic.

11:15 AM – 11:30 AM Closing Comments

11:30 AM Departures
Boxed Lunches Available


END OF CONFERENCE

For additional information contact:

FASEB Science Research Conferences
9650 Rockville Pike
Bethesda, MD 20814

italy@faseb.org


www.faseb.org/src