Hartwell Center for Bioinformatics and Biotechnology Clayton W. Naeve, PhD

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Oct 23, 2013 (3 years and 11 months ago)

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Hartwell Center for Bioinformatics

and Biotechnology


Clayton W. Naeve, PhD

Director


t


901.595.3
861



f


901.595.
8600

clayton.naeve
@stjude.org

October 3
, 201
2


Paul Thomas
, Ph.D.

Department of
Immunol ogy

St. Jude Chi l dren’s Research Hospi tal


Dear
Paul,


We woul d be del i ghted to provi de support for your proposed studi es
.

S
peci fi cally
, we wi l l perform

genotypi ng
anal ysis

of candi date genes and/or
whol e
-
genome scanni ng to i denti fy nucl eoti de vari ants associated wi th
response to i nfl uenza i nfecti on.

As
descri bed bel ow

the
Hartwel l Center for Bi oi nformati cs & Bi otechnol ogy

i s wel l
equi pped to perform hi
gh
-
throughput genotypi ng anal ysi
s
.

Our genotypi ng pl a
tforms provi de great fl exi bi l ity i n
assay desi gn, rangi ng from smal l l ists of custom SNPs to genome
-
wi de fi ne mappi ng usi ng hi gh densi ty SNP arrays.
Furthermore, our servi ces are hi ghl y cost effecti ve

due to
our

modest
chargeback
fee
s
whi ch
are

based onl y

on the
cost of
consumabl es used i n
an i nvesti gator’s

experi ment.


The Hartwel l Center has several pl at
forms for genotypi ng anal ysis.

For custom mi d
-
pl ex anal ysis
(44 to 384
-
pl ex
SNPs)
we use the Il l umi na Gol denGate assay whi ch i s supported by the BeadX
press system.
Typi cal ly, the pri ce per
sampl e ranges from $15 to $30 on the BeadXpress

system
.
For hi gher
custom
mul ti
-
pl exi ng, w
e
use

the Il l umi na
Gol denGate assay
on

the Hi Scan
BeadArray
system
. The BeadArray pl atform provi des custom genotypi ng opti on
s
from ~1
,
500 SNPs to >100,000 SNPs

wi th pri ci ng varyi ng from $50 to $200 per sampl e
.

For genome
-
wi de SNP
anal ysis we support both the Il l umi na Infi nium and Affymetri x
SNP
pl atforms.
Arrays

supported on these pl atforms
range from ExomeSNP

anal ysis ($39
-

$52 per sampl e), to whol e
-
genome scanni ng usi ng 1



5 mi l l i on SNPs. Pri ci ng
on the genome
-
wi de arrays starts at $235 per sampl e (eg Affymetri x SNP 6.0 and Il l umi na Omni 2.5).
To ensure
success i n your studi es, our staff i s hi ghl y trai ned

and has enormous experti se on al l pl atforms. To date, our faci l ity
has processed over 25,000 sampl es for genotypi ng anal ysis.


The Hartwel l Center i s a uni que i ntegrati on of hi gh
-
throughput bi otechnol og
y and bi oi nformati cs resources
desi gned to provi de
St. Jude
researchers wi th state
-
of
-
the
-
art tool s for di scovery.
T
he Hartwel l Center, through i ts
expert staff and si gni fi cant resources, i s pl eased to offer these capabi l iti es i n support of your proposed
i nvesti gati on i nto the geneti c basi s of
response to

i nfl uenza i nfecti on
.



Si ncerel y
,




Geoffrey Neal e, Ph.D.


Administrative Director
-

Genomics

Hartwell Center for Bioinformatics & Biotechnology

t

901.595.3284

f

901.595.2945

geoffrey.neal e@stjude.org