INTERNATIONAL SCHOOL OF ENGINEERINGS

polarbearfellowshipBiotechnology

Dec 12, 2012 (4 years and 10 months ago)

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Dolly Montoya C. Universidad Nacional de
Colombia.

1

International School of
Engineers

Second Assembly of the RCI: 21st to 24th
November 2007 in Forbach

Dolly Montoya C,
PhD, MSc,



www. ibun.unal.edu.co

Dolly Montoya C. Universidad Nacional de
Colombia.

2

Engineering in Biotechnology
-

Biotechnology in Engineering

Structure

1.

Introduction

2.

Biotechnology Innovation strategies

3.

The overall strategy for developing

biotechnology

4.
New needs in Engineering for
developing biotechnology

5.
Multilateral network for increasing
biotechnology's competitiveness and a
proposal for future developments


Dolly Montoya C. Universidad Nacional de
Colombia.

3


Introduction


Dolly Montoya C. Universidad Nacional de
Colombia.

4

International School of
Engineers


proposal from Metz School of Engineers



Cartagena Network

Gesticon




PUEDE (University Enterprise for development programme
)



International School of Engineers

Dolly Montoya C. Universidad Nacional de
Colombia.

5

2. Biotechnology
Innovation
strategies


Dolly Montoya C. Universidad Nacional de
Colombia.

6





Innovation life
-
cycles

We have been given an opportunity to become
part of the development of a new world
economy via interdisciplinary innovation

Dolly Montoya C. Universidad Nacional de
Colombia.

7


Biotechnology




This is a group of techniques
using live organisms or
substances coming from such
organisms for:


making or modifying a product;


improving plants or animals; and


developing microorganisms for
specific uses

Dolly Montoya C. Universidad Nacional de
Colombia.

8




It is knowledge or “know
-
how” intensive




It is interdisciplinary




Traditional processes are upgraded using current
biotechnology





It embraces different production sectors




Biotechnology
Characteristics


Dolly Montoya C. Universidad Nacional de
Colombia.

9

Biotechnology is interdisciplinary

Biology
and Pure
Science

Engineering

Economics

Law and
Political
Science

Human
science

Biotechnology

Dolly Montoya C. Universidad Nacional de
Colombia.

10

Industrial sectors
influenced by
biotechnology

Pharmaceutical
biotechnology

New substances

Industrial biotechnology

New raw materials

Wastewater treatment

Dolly Montoya C. Universidad Nacional de
Colombia.

11

Biotechnology’s products

Traditional biotechnology


High
-
volume and low
-
value

Methane, ethanol, biodiesel, biomass,
waste water purification, residue
treatment, amino acids, organic acids,
foods, acetone, buthanol, yeasts,
polymers, etc

Biotechnology in the era of
genetic engineering


Low
-
volume and high
-
value

Products obtained by genetic
manipulation, gene and non
-
gene therapy, genetically
modified plants

Biotechnology in the post
-
genomic era

Transcriptomics, proteomics,
metabolomics, bioinformatics and
nanobiotechnology


Biología sintética

Synthetic biology: the
future

Using highly complex computational
models for designing and constructing
metabolic pathways or even partially
or totally synthetic microorganisms

Dolly Montoya C. Universidad Nacional de
Colombia.

12




Knowledge
is the key to sustainable
growth; however, investment in
research and development (R&D) is
not enough for producing innovation.
We can only talk of innovation if it is
accompanied by economic growth and
social wellbeing.

Biotechnology Innovation
strategies

Dolly Montoya C. Universidad Nacional de
Colombia.

13

Biotechnology Innovation
strategies


Knowledge (know
-
how)


Skills development


Promoting a setting for innovation


Strengthening an innovative
community

Dolly Montoya C. Universidad Nacional de
Colombia.

14

Transforming higher
education represents a


challenge for the





present

Dolly Montoya C. Universidad Nacional de
Colombia.

15

Long
-
term view

The

changing

international

setting


International

treaties

and

interdependent

relationships
.



New

relationships

and

changing

roles

between

university
-
company
-
state,

new

actors
.


Widening

the

economic

and

technological

breach

between

countries

and

regions
.


The university’s long
-
term view must take the national and international setting
into consideration:


New

paradigms

for

the

society

of

knowledge

(know
-
how)


Educational
:

the

university’s

new

role

as

a

producer

of

know
-
how

companies
.


Technological
:

investment

in

technological

development

multiplies

economic

development
.


Organisational
:

u
sing

strategic

thinking

for

resolving

complex

problems
.

Ongoing

innovation

in

all

areas
.


Social
:

knowledge

reduces

the

social

gap

and

produces

wellbeing
.

Regarding the challenges of biotechnology

Dolly Montoya C. Universidad Nacional de
Colombia.

16

Transforming higher education



represents



a challenge for the present

University

Society

Feedback


Teaching


Investigation


Extension

Knowledge

Dolly Montoya C. Universidad Nacional de
Colombia.

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3.0
The overall strategy for
developing Biotechnology




Engineering in Biotechnology
-

Biotechnology in Engineering



Dolly Montoya C. Universidad Nacional de
Colombia.

18


The overall strategy for
developing Biotechnology


Developing the ability to formulate
strategic plans


Subscribing to modern management
techniques



Strengthening entrepreneurial
decision
-
making



Developing an educational policy aimed at
biotechnological education/training

Dolly Montoya C. Universidad Nacional de
Colombia.

19

Engineering in Biotechnology
-

Biotechnology in Engineering


4.0 New needs in Engineering for
developing Biotechnology



Engineering’s contribution to the
future of Biotechnology



Dolly Montoya C. Universidad Nacional de
Colombia.

20

The cell

Red Biotechnology
Human
Biotechnology


White Biotechnology

Industrial Chemistry
Biotechnology


Green Biotechnology

Agriculture
Biotechnology

Dolly Montoya C. Universidad Nacional de
Colombia.

21

Engineering’s contribution to





traditional biotechnology

Isolation and
characterisation

Laboratory

Scaling
-
up

Support devices:



Orbital shakers,



Refrigerators,



Anaerobic and
aerobic incubators,



Anaerobic chambers,



pH meters, etc



Non
-
structured
growth k
inetics models



Blended and oxygenation k
inetics in reactors



Instrumentation
and control

systems in
bioprocess



Enzyme

immobilisation



Bioreactor design



Cellular protein p
roduction

Dolly Montoya C. Universidad Nacional de
Colombia.

22

Engineering’s contribution to
biotechnology in the era of genetic
engineering

Isolation,
characterisation

and gene
modification

Laboratory

Scaling
-
up

New technologies:




Recombinant DNA




Hybridome technology





Cell culturing





DNA and protein
sequencing





Bioremediation



Metabolic engineering





Molecular modelling





Protein
chromatography




New separation processes:
nanofiltration, ultrafiltration, molecular
sieves, supercritical fluid extraction,
azeotropic distillation, etc

Dolly Montoya C. Universidad Nacional de
Colombia.

23

Probing microbial communities

at environmental,

community, cellular, sub
-
cellular and molecular levels

Dolly Montoya C. Universidad Nacional de
Colombia.

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Dolly Montoya C. Universidad Nacional de
Colombia.

25

Dolly Montoya C. Universidad Nacional de
Colombia.

26

Synthetic Biology


Traditional Biochemistry and molecular
research methods to involve to a degree of
maturity and they can used for redesigned
live cells, biochemical interaction and
metabolic routes.


Rapid DNA synthesis must also used for
constructing artificial genes and operons,
leading to thinking about completely
sythetic live organisms

Dolly Montoya C. Universidad Nacional de
Colombia.

27

The Biotechnology of the first half of
the 21
st

century must face great
challenges

20
th

century: post
-
genomic era and the
search for structures

Genomic,
Proteomic,
Metabolomic,

Transcriptomic


The cell as the
sum of its
parts

21
st

century: deep
understanding of biological
systems ensuring their
efficient use

Synthetic biology

Bioinformatics, systems
and control

theories, complex mathematical
models, c
omputational s
imulation,
robotics, mechatronics, etc.

Dolly Montoya C. Universidad Nacional de
Colombia.

28

5.0 Multilateral network for
increasing biotechnology's
competitiveness and a
proposal for future
developments



Engineering in Biotechnology
-
Biotechnology in Engineering





Dolly Montoya C. Universidad Nacional de
Colombia.

29

Proposal for cooperative
work

Biotechnological core process

(BCP)


Organisation:
the Biotechnology Network (BIONET)




Highly qualified scientists and professionals
i
n areas related to biotechnology


Physical

i
nfrastructure


R&D interaction


Developing prototypes with enterprises


Ongoing evaluation


Biobusiness

Dolly Montoya C. Universidad Nacional de
Colombia.

30

Immediate tasks



We have to know whom we are going
to work with in biotechnology and
engineering (establishing the network)


The areas of interest in biotechnology
must be defined and clearly focused


A coherent strategic plan must be
drawn up


Specific projects must be defined


Dolly Montoya C. Universidad Nacional de
Colombia.

31





Infrastructure

Prototype
development
with companies

R&D
interaction

Ongoing
evaluation

Scientists,
technicians

and highly

qualified
professionals



Biobusiness

Biotechnological core process

(BCP)

Dolly Montoya C. Universidad Nacional de
Colombia.

32

The Institute of Biotechnology




at the

Universidad Nacional de Colombia


An organisation
which learns



University
-

business
relationship

Dolly Montoya C. Universidad Nacional de
Colombia.

33


Points of rapprochement in

University


Business

University

Incubator

-

CDT

Business

Knowledge

Producing new ideas


Defining intellectual
property

Defining standards for
biosafety and the
environment

Establishing ethical and
legal standards

Evaluating national and
international ideas regarding
products and services

Accompaniment in
negotiation

Links for technological
development and prototypes

Production and sales

Market niches

Business plans

Industrial scaling
-
up

Dolly Montoya C. PhD

Instututo de Biotecnología

Dolly Montoya C. Universidad Nacional de
Colombia.

34


Industrial

development


Sustainable exploitation

of biodiversity and

genetic resources


Bioprospecting:

consolidating the

system in the search

for desired

biological activities


Establishing criteria

for defining promising

activities in each

case

Taxonomic definition of

species by biochemical

and molecular characterisation


If necessary, improving

strains via

metabolic engineering or

genetic transformation

Seeking a partner

for scaling
-
up the process

at pilot
-
plant level

Fermentation at

bank level

If proving viable,

Initiating the

patenting process

Drawing up a business

plan from the

fermentation results



Preliminary

technical
-
economic

evaluation

From the laboratory to industry


Dolly Montoya C. Universidad Nacional de
Colombia.

35

Bioprocesses and Bioprospecting groups


What do we do in
common?

Bioprospecting

Molecular and
enzymatic
characterisation

Solventogenics

Rubber

Yeasts

Biofertilisers
and PHAs

Solventogenics

Rubber

Yeasts

Biofertilisers
and PHAs

Fermentation

Solventogenics

Rubber

Yeasts

Biofertilisers
and PHAs

Pilot
-
plant

Microbiology laboratory


Fermentation and Enzyme
laboratories

IBUN biofertilisers

MAVALLE rubber

PROCAPS yeasts

Dolly Montoya C. Universidad Nacional de
Colombia.

36

Isolating

solventogenic




Clostridia

155 soil
samples

178 Clostridia
isolates

13 promising
strains

must be
characterised

Dolly Montoya C. Universidad Nacional de
Colombia.

37

Ácidos grasos

Biodiesel

GLICEROL

Bioprospecting for

1,3 propanodiol production

Dolly Montoya C. Universidad Nacional de
Colombia.

38

Biopolymer bioprospecting

Strain

Milling and packaging

Drying

Precipitation

Reacting

Obtaining enzyme

Fermentation

Characterisation

Isolation

Soil

Dolly Montoya C. Universidad Nacional de
Colombia.

39

SIVAN biopolymer
production pilot
-
plant


Dolly Montoya C. Universidad Nacional de
Colombia.

40

Bringing on line

Fermentation reaction

Recuperation

Receiving the polymer

Precipitation

Drying

Dolly Montoya C. Universidad Nacional de
Colombia.

41

Biofertiliser production pilot
-
plant


Dolly Montoya C. Universidad Nacional de
Colombia.

42

Biofertilisers and Biocontrollers


Containing micro
-
organisms
which
increase nutrient supply
and availability
, thereby
improving soil fertility level and
contributing towards plant
growth

The products so formulated have been initially
tested on 40,000 hectares sown with
rice and
cotton

The dose of nitrogen and phosphor from
inorganic sources applied to rice crops
was reduced by 35% with consequently
reduced costs, thereby improving the
crop’s profitability

Fosfosol

Registro ICA No 4717


Dimargon

Registro ICA No 4716

Dimazos

Registro ICA No 4718


Trifesol


Pilot
-
plant (
100 and 1,000L
)

Mention of honour

in the First National
Competition for Projects in Agribusiness
Biotechnology having entrepreneurial
potential

Packaging

Selecting micro
-
organisms

Producing the

active ingredient

Field
validation
trials

Formulating

Dolly Montoya C. Universidad Nacional de
Colombia.

43

E
-
mail: dmontoyac@gmail.com