Part II - Punjabi University Patiala

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Dec 1, 2012 (4 years and 11 months ago)

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Proposed Syllabus

M.Sc. Microbial and Food Technology

(Semesters III & IV)

Sessions 2009
-
2010, 2010
-
2011


PAPER
-

IX

ENZYMOLOGY and ENZYME TECHNOLOGY

M. marks : 80





Lectures to be delivered : 60

Time allowed : 3 Hours


INSTRUCTIONS FOR THE PAPER SETT
ERS


The question paper will consist of five sections A, B, C, D and E. Section
-
A, B, C and D
will have two questions from the respective sections of the syllabus and carry 15 marks
each. Section

E will consist of 10 short answer type questions which will

cover the
entire syllabus uniformly and will carry 20 marks in all.


INSTRUCTIONS FOR THE CANDIDATES


1.

Candidates are required to attempt one question each from sections A, B, C and D
of the question paper and the entire SECTION E.

2.

The use of scient
ific calculators is allowed.


SECTION
-
A

1.

Enzymes: structure, evolution and its basis.

2.

Enzyme action: specificity, molecular aspects of enzyme action, and examples
from different classes of enzyme.

3.

Regulation of enzymic action: activation of enzym
es, covalent modification,

allosteric interaction, multienzyme complexes.

.

SECTION
-

B

4.

Industrial production of enzymes: sources of enzymes, criteria for the selection of

source for enzyme production, methods of large scale production
-
solid substrate

fermentation and submerged fermentation, factors affecting enzyme production,

amylases, cellulases, pectinases, lactases, invertases, lipases, proteases etc.

5.

Immobilization of biocatalysts: definition, objectives and advantages of
immobilization; techniqu
es of immobilization, matrices
-
types, advantages and
limitations.

6.

Kinetic characterization: immobilized enzymes
-
comparative account of
immobilized biocatalysts, concept of co
-
immobilization.


SECTION
-
C


7.

Biochemical applications: role of soluble and immobi
lized enzymes in the
synthesis and production of amino acids and chiral compounds; use of enzymes as
detergents.

8.

Pharmaceuticals: role of soluble and immobilized enzymes in production of
antibiotics, steroids, and other important intermediates of biotechno
logical
industry; role of soluble and immobilized enzymes in diagnosis and treatment of
diseases; enzyme therapy.

9.

Analytical applications: theory and applications of various enzyme electrodes e.g.
enzyme sensors, enzyme membranes, biochips/bio
-
semiconducto
rs etc.

10.

Applications in Food
-
industry: soluble and immobilized enzymes
-
food production
and processing, amylases, pectinases, proteases, lipases, glucoisomerases,
naringinase.


SECTION
-
D


11.

Enzyme engineering :

in
-
vitro

approaches to improve functional effici
ency.

12.

Recombinant enzymes: overproduction and applications.

13.

Enzymes in organic solvents: modes of using enzymes, fundamentals and new
properties.


RECOMENDED READING

1.

Fundementals of Enzymology: The cell and molecular biology of catalytic
proteins by NC Pri
ce and L Stevens: Oxford University, 2000.

2.

Enzymolgoy lab Fax., edited by P.C. Engel, Academic Press, 2003.

3.


Enzyme Structure and Function, 2nd edition by A, Fersht, W.H. Freeman
and Co., NY, 1999.

4.

Enzymes, Biomass, Food and Feed (Biotechnology 2E,
Vol.9) Ed. Rehm, Reed,

A.Phuler, Wiley
-
VCH, Berlin, 2001.

5.

Enzyme Kinetics: Principles and Methods by Hans Bisswanger and Leonie
Bubenheim, April 2002.

6.

Industrial Enzymology: The application of Enzymes in Industry edited by T.

Godfrey and S. May. Mc
Millan publishers, 2001.

7.

Enzyme Technolog by M.F. Chaplin and C. Bucke, Cmabrigde University Press,

NY, 1990.

8

Enzymes in Food Processing , 2nd Edition, Edited by G A Tucker and L F J
Woods.

10.

Manual of Industrial Microbiology and Biotechnology 2nd
edition, edited by A.L.

Demain and

N.A. Soloman, American Society for Microbiology Washington,

2003.

11. A Textbook of Industrial Microbiology 2nd edition (Biotechnology) by W.Cruger

and A.Cruger,

Sinauer Associates, Sunderland,US, 2004.

12. Enzymatic

reactions in organic media Ed Munishwar N Gupta 2000.

Birkhauser
Verlag publication.

13

Supramolecular enzyme organization Quaternary structure and beyond by Peter

Friedrick, Pregamon Press, Oxford, 1994.

Paper
-
X


MICROBIAL AND FOOD TECHNOLOGY


M. Marks

: 80




Lectures to be delivered : 60

Time allowed : 3 hours


INSTRUCTIONS FOR THE PAPER SETTERS

The question paper will consist of five sections A, B, C, D, and E. Section A, B, C and D
wi
ll have two questions from
the respective sections of the syllabu
s and carry 15 marks
each. Section E will consist of 10 short answer type questions which will cover the entire
syllabus uniformly and will carry 20 marks in all.


INSTRUCTIONS FOR THE CANDIDATES

1.

C
andidates are required to attempt one question each from se
ctions A, B, C and D
of the question paper and the entire section E.

2.

The use of scientific calculators is allowed.


Section


A

1.

microbial transformation: types of transormation, applications in biotechnology
and food technology, industry.

2.

Pharmaceutical pr
oducts: fermentative production of antibiotics,
penicillins,cephalosporins, erythromycins, vancomycins and streptomycin etc.

3.

vaccines: fermentative production of vaccines, recombinant vaccines and steroid
transformation.


Section


B

4.

production of food add
itives and ingredients: fermentative production of amino
acids
-
L
-
glutamic acid and L
-
aspartic acid.

5.

production of vitamins: thiamin (B
-
1), riboflavin (B
-
2), vitamin (B
-
12).

6.

microbial polysachharides: fermentative production of xanthan gums, dextrins,
cyclo
dextrins etc.


Section


C

7.

food and beverages: production technology of cheese, bread and Indian fermented
foods.

8.

fermented dairy and sugar products: production of cultured dairy milk, cultured
cream, yougurt, kefir, cane syrups, invert syrups, levulose sy
rup, high fructose
syrup, maltose syrup.

9.

commercial status of specialized cells: current status of applications of
immobilized cells in fermentative industry.


Section


D

10.

food preservation: preservation of foods
-

physical (dehydration, freeze
-
drying,
heat
, irradiation) and chemical (antibiotics, organic acids, nitrates and nitrites,
sulphites and sulphur dioxide etc.)

11.

food regulations: standardization of fermented and processed foods.

12.

by
-
product utilization: technology for the utilization of fermented/food

industry
by products
-
whey, whey bits, waste from fruit and vegetable processing industry
etc.


Recommended Reading.


1.

Prescott and Dunn’s industrial microbiology by B. Reed McMillan Publishers
Ltd., Connecticut, 1982.

2.

Principles of fermentation technology
by P. F. Stan bury, A, Whitaker and Hall,
Pergamon Press, NY, 1984.

3.

Comprehensive Biotechnology by Moo Young (Vol. 3 and 4), Pergamon Press,
NY, 1985.

4.

Fundamentals of Biotechnology by P. Praive, B. Fraust, W. Sitting and D. A.
Sukatesh WCH Weinheim, 1987.

5.

Biotechnology, Principles and Applications by J. Higgins, D. J. Bestand j. Jones,
Blackwell Scientific Publications, London, 1985.

6.

Biotechnology by R. H. Rehm and G. Reed (Vol 4, 5, 6 and 7a), Verlag Press,
NY, 1982 and 1987.

7.

Essays in applied microbiology

by J. R. Norris and M. H. Richmong: John Wiley
and Sons, NY, 1981.

8.

Yeast Biotechnology by D. R. Berry, I. Russel and G.G. Stewart: Allen and
Unwin, Boston, 1987.

9.

Microbial Biotechnology, Fundamentals of Applied Microbiology by A. N.
Glazerand H. Nikaido,
W. H. Freeman and Co., NY, 1995.

10.

Biotechnology: Food Fermentation Technology by V. K. Joshi and A. Pandey,
Educational Publishers and Distributers, New Delhi, 1997.

Paper
-

XI

ENVIRONMENTAL
BIO
TECHNOLOGY
/

ENVIRONMENTAL
TECHNOLOGY

(BT/MFT)




M. Marks: 80







Lectures to be delivered: 60

Time allowed: 3 hours



INSTRUCTIONS FOR THE PAPER SETTERS

The question paper will consist of five sections A, B, C, D, and E. Section A, B, C and D
will have two questions from from the respective sections of the sylla
bus and carry 15
marks each. Section E will consist of 10 short answer type questions which will cover the
entire syllabus uniformly and will carry 20 marks in all.


INSTRUCTIONS FOR THE CANDIDATES


1.

Candidates are required to attempt one question each from

sections A, B, C and D

of the question paper and the entire section E.


2.

The use of scientific calculators is allowed.



Section


A


1)

Introduction to environment, nature of pollutants, biochemical and physiological
effects of pollutants on plants, animals

and microbes, biomarkers.

2)

Environmental applications of biotechnology: municipal waste treatment, heavy
metal contamination, low molecular weight organic compounds, mineral leaching,
microbial enhanced oil recovery, bioremediation, biological deodorizatio
n.

3)

Principles of Treatment: laboratory and equipment, design for containment of
biohazards, stoichiometry and kinetics.


Section


B


4)

Treatment of liquid and solid wastes
-
aerobic techniques, bioleaching and bio
-
mining
-
microbiology, biochemistry and applica
tions

5)

Aerobic treatment technologies: activated sludge (AS), Completely mixed
conventional activated sludge (CCAS), contact stabilizer (CSAS), step aeration
(SAAS), completely mixed (CMAS), extended aeration (EAAS), pure oxygen or
high purity oxygen (POAS)
, tapered aeration (TAAS) systems.

6)

Aerated lagoons: aerobic and facultative tricking filters and rotating biological
contactors, sludge characterization, aerobic land based treatment systems, land
farming and composting and vermicomposting.

Section


C


7)

Tr
eatment of liquid and solid waste: anaerobic technologies: anaerobic
decomposition, anaerobic filter reactor, anaerobic contact reactor, fluidized bed
reactor, up
-
flow anaerobic sludge blanket (UASB), anaerobic baffled reactor,
mechanism of anaerobic ferme
ntation, biodegradation of organic compounds,
factors affecting process operation.

8)

Bio
-
energy: bio fuel cells, fuel alcohol production, hydrogen gas production,
biogas technology raw materials, biochemistry, microbiology, biogas plant,
factors affecting bi
ogas production and its status in India.

9)

Treatment of waste air: biological technologies of waste air treatment


Section


D


10)

Biosensors: definition, advantages and disadvantages of biosensor,
immobilization strategies for the construction of biosensors, t
ypes of transducers,
Development of Biosensors for heavy metal ions, BOD biosensor

11)


Construction of biosensors and kits for pathogens, insecticides and pesticides.
Immuno biosensors

12)

Protein engineering and construction of generic biosensors. Modification a
t DNA
level, modification of polypeptide chain

(i)

Zinc finger, carbonic anhydrate, CAM, CAMBP

(ii)

Engineered antibodies and Ion channels

Modifications that increase the activity, modification that alter the surface
properties, modification that alter specificity,

coenzyme attachment, new enzyme
activities


Recommended reading

1.

A text book of biotechnology by R. C. Dubey. S. Chand & company
Ltd.. New Delhi. (2002).

2.

Indutrial water pollution Control, W. Wesley El
benfields
-
jr. Mc graw Hill
international Editions (1998).

3.

Biotechnology
-
Applications to environmental protection. First Edition
1993, Published by Mo Mera Pandey, for Himalaya Publishing House.

4.

Advances in Industrial waste water treatment edited by P. K. G
oel,
Technoscience publications, First Editions, 1999.

5.

Commercial Biotechnology
-

An by Elsevier Science publishers B. V.,
Amsterden, The Newdesk, 1984.

6.

Environmental Engineering Howard and Peevy, Donald R. Rowe and
George Technologies, Mc Graw Hill Interna
tional Edition (1988).

7.

Protein Immobilization: Fundamentals and applications edited by Richard
F. Taylor, 1991.

8.

Basic Environmental Science by G. S. P. Iyer, Educational Publishers and
Distributers, New Delhi, 1997.

9.

Comprehensive Biotechnology by M. Moo
-
Yo
ung (Vol. 4). Pergamon
Press, New York, 1985.


Paper
-
XII


IPRs, TQM, BIOSAFETY AND

COMMERCIAL BIOTECHNOL
OGY


M. Marks : 80




Lectures to be delivered : 60

Time allowed : 3 hours


INSTRUCTIONS FOR THE PAPER SETTERS

The question paper will consist of five

sections A, B, C, D, and E. Section A, B, C and D
will have two questions from from the respective sections of the syllabus and carry 15
marks each. Section E will consist of 10 short answer type questions which will cover the
entire syllabus uniformly an
d will carry 20 marks in all.


INSTRUCTIONS FOR THE CANDIDATES

1.

C
andidates are required to attempt one question each from sections A, B, C and D
of the question paper and the entire section E.

2.

The use of scientific calculators is allowed.

Section


A

1.

Percep
tions to Biotechnology:

Social aspects of Biotechnology, status of
biotechnology in India and Punjab; Development and assistance: university
-
industry relationships in Biotechnology, Government programmes in
development of biotechnology: role of internation
al organizations in
development of biotechnology.

2.

Intellectual property rights : Intellectual property, types and related Indian
legislations; Intenational conventions: TRIPs, PCT, Paris convention,
Budapest treaty, UPOV; preparation and preservation of in
vention records;
Intellectual property management.

3.

Patents: Patentability criteria, Patenting system in India, USA and Europe,
patentability of biological processes and products in India, US and Europe.
Plant Variety protection, Criteria for registration o
f new varieties in India.
Trade Secret protection,


Section


B

4.

Total

quality management: introduction, concept, role and its importance;
contributions of management thinkers in quality delivery market.

5.

C
ore concepts of TQM: quality of profit, cost and eco
nomics of quality,
competitive bench market and quality delivery market.

6.

T
ools and techniques of TQM: techniques for analyzing quality process,
statistical process control, problem solving tools.






Section


C

7.

Information systems and technologies: basi
c concept, types, information
systems and organizations
.

8.

Information Management

and decision making.

9.

E
nsuring quality with information systems: computer information process in
designing the organization with information systems.

Section


D

10.

International O
rganization for Standardization (ISO)
-

About the organization
and its functioning, history and relevance of certification.

11.

ISO 9000 family of standards
-

members, various revisions and requirements.

12.

ISO 14000 family of standards
-

members,various revisions
and requirements.



Recommended Readings

1.

Total quality in research and development by G. C. Mclauhlin.

2.

Guide to Total Quality Management by Ralph early.

3.

Total Quality Management by Feighan Baun.

4.

Total Quality Management by Duncan.

5.

Total quality in informat
ion systems and technology by J. Woodali.

6.

Guide to Quality Management systems for the food industry by R. Early, Blackie
Academic, NY, 1995.


PRACTICAL PAPER V

Pertaining to:

The
ory
paper XIII : Enzymology and Enzyme Technology

Theory paper XII : Micr
obial and Food Technology


Max. Marks: 100





Practical Time: 60 hours

Time: 4 hours




1.

Standardization and optimization of immobilization techniques for enzymes and
cells.

2.

Comparative kinetic characterization of soluble/free and immobilized
enzymes/cells
.

3.

Production of enzymes at laboratory scale
-

alpha amylase, protease, pectinase etc.

4.

Demonstration of activities soluble and immobilized enzymes
-

invertase, alpha
amylase, protease, pectinases and naringinase.

5.

Fabrication of enzyme sensors and demonstratio
ns of their functions.

PRACTICAL PAPER VI

Pertaining to:

The
ory
paper XI : Environmental technology

Theory paper XII :
IPR,
Total Quality management
, Biosafety

and Commercial

Biotechnology






Max. Marks: 10
0





Practical Time: 60 hours

Time: 4 hours



1.

Isolation of phage from sewage.

2.

Separation of pesticides by HPLC method.

3.

Determination of heavy metal ions in industrial effluents by Atomic absorption
spectrophotometer and spectrophotometric methods.

4.

Charact
erization of industrial effluents for pH, TS, TDS, TSS, alkalinity and
hardness parameters.

5.

Evaluation of population potential of waste materials as Biochemical Oxygen
Demand (BOD).

6.

Determination of chemical oxygen demand (COD) in various effluents.

7.

Develo
pment of biosensor (enzyme based/ microbe based) for monitoring heavy
metal ion/pesticides in the environment.

8.

Testing of xenobiotic degradative abilities of microorganisms.

9.

Demonstration of methane production from industrial effluents using batch and
anae
robic baffled reactors.

10.

Biogas production monitoring by gas chromatography.

11.

Bioremediation of heavy metal laden (Ni+2) industrial effluent by immobilized
cyanobacteria through packed bed reactor.

12.

Bioresin for treatment of chromium
-
laden effluents.

13.

Use of
MS
-

Office software to sketch a company profile

14.

Emulation of Simon`s Model of decision making for a fictitious concern

15.

An over view of ISO 9000 family of Quality Management Standards

16.

A brief about ISO 9000 certified Indian companies

17.

An overview of ISO 1400
0 family of Environment Management Standards

18.

A brief about ISO 14000 certified Indian companies

19.

To identify the TQM related problems in an organization and to give a possible
solution.

20.

To study the essential elements of TQM.

21.

To demonstrate the development
of a small
-
scale vermicomposting bin for
commercialization.


Paper XIII

Bioinformatics and Computation Biology

.


M. Marks : 80




Lectures to be delivered : 60

Time allowed : 3 hours


INSTRUCTIONS FOR THE PAPER SETTERS

The question paper will consist of

five sections A, B, C, D, and E. Section A, B, C and D
will have two questions from from the respective sections of the syllabus and carry 15
marks each. Section E will consist of 10 short answer type questions which will cover the
entire syllabus uniform
ly and will carry 20 marks in all.



INSTRUCTION FOR THE CANDIDATES


1.

Candidates

are required to attempt one question each from SECTIONS A, B,
C and D of the question paper and the entire SECTION E.

2.

The use of scientific calculator is allowed.


Section A

1.

Fu
ndamentals of computers: Definition of computers, computer organization,
memory, number systems, input and output devices. Types of computers, network
computers, working wit
h mainframe, personal computers, ftp, http, web, internet
and intranet, web browsin
g.

2.

Operating system: Windows and Linux:

3.

Programming language C: character set, identifiers and keywords data types,
operator and expression control statements, switch break, continue statements,
function arrays, pointers.


Section B


4.

Introduction to Bioin
formatics: what is bioinformatics,
use of computer for
biological research, o
verview of web servers and software: NCBI, EBI,
Expasy,
PDRB,

5.

Search Engines: role in biological research, PubMed, ENTREZ, SRS


6.

Overview of databases flatfile formats: classifica
tion of biological databases,
primary nucleic acid and protein sequence databases: EMBL, GenBank, DDBJ,
SWISS
-
PROT and TrEMBL
, PIR
-
PSD, PDB; secondary nucleic acid and protein
databases: Unigene, REBASE,
ENZYME
, PRINTS, SCOP, CATH
.





Section C


7.

Computati
on Biology: Analysis of Nucleic acid and protein sequences,

homology
analysis, ePCR, ORF finder, restriction pattern analysis, gene prediction;
analysis
of protein sequences: homology analysis, prediction of 2D and 3D structures;

sequence comparison algor
ithm, sequence scoring schemes, BLOSUM

62
, PAM
250.

8.

Sequence and Genome analysis: Local alignment, global alignment, FASTA,
BLAST (Blast P, Blast N, Blast X) and similarity searching scores and their
statistical interpretation.

9.

Functional genomics strateg
ies for generating EST and full length inserts, EST
clustering and assembly, statistical analysis of EST and EST data, microarray.


Section D


10.

Functional Proteomics: Protein family and domain databases (PROSITE, Pfam,
SMART), algorithms for multiple seque
nce alignment (CLUSTAL x and
CLUSTAL w) and comparison with sequence databses. Biological motifs
(PSSMs, HMMs), finding protein
-
coding regions.

11.

Prediction of macromolecular structure (protein secondary and tertiary structures)
and properties, software for
displaying macromolecular structures (ProtParam,
PREDATOR).

12.

Protein modeling software: RASMOL, MOLMOL, INSIGHT, EMBOSS, and
their applications



RECOMENDED READING



1


Introduction to Bioinformatics By A M Lesk, Oxford University Press, USA,

2005

2.

Bioinformatics Basics: application in biological science and medicine. By
Hooman Rashidi and L K Buehler, CRC Press 2005.

3.

Proteins and Proteomics: a lab manual By R J simpson, Cold Spring Harbor,
US 2003.

4.

Bioinformatics: Genes, proteins and Computers By C
A Orengo and J M
Thornton, Taylor and Francis, US 2003.

5.

Bioinformatics Computing By Bryan Bergeron, Pearson Education, US 2002.

6.

Bioinformatics: sequence and genes analysis by Mount, D A .2001.

7.

Bioinformatics: methods and Protocols by Baxevanis, A.D.2001.

8.

I
ntroduction to protein Architecture: The structural biology of proteins By A
M Lesk, Oxford University Press, UK, 2000.

9.

Bioinformatics: A practical guide to Analysis of genes and proteins 2
nd

ed.

10.

Let us C by Y. kanitkar, BPB Publications.

11.

Computer Fundam
entals by P. K. Sinha
.

PAPER
-
XIV

TISSUE AND CELL CULTURE TECHNOLOGY


M. Marks : 80




Lectures to be delivered : 60

Time allowed : 3 hours


INSTRUCTIONS FOR THE PAPER SETTERS

The question paper will consist of five sections A, B, C, D, and E. Section A,
B, C and D
will have two questions from from the respective sections of the syllabus and carry 15
marks each. Section E will consist of 10 short answer type questions which will cover the
entire syllabus uniformly and will carry 20 marks in all.

INSTRUCTIO
NS FOR THE CANDIDATES

1.

Candidates are required to attempt one question each from Sections A, B, C and D
of the question paper and the entire Section E.

2.

The use of scientific calculators is allowed.

SECTION
-
A

1.

Plant tissue culture : history of plant
cell culture, culture media
-
composition,
preparation and development, cellular totipotency, cryopreservation.

2.

Callus and cell culture : Isolation of cells, growth of single isolated cells.

3.

Suspension culture : regeneration and maintenance of callus,
organogenesis and
embryogenesis.

SECTION
-
B

4.

Organ culture : meristem culture, embryo culture and embryo rescue, anther
culture, virus free plant production and haploid plant production, production of
synthetic seed, micropropagation.

5.

Protoplast cultur
e and fusion : isolation of protoplasts, culture and regeneration;
fusion of protoplasts, selection of fusion products of protoplasts; cybrids.

6.

Somaclonal variation, instability and somaclonal variations, plant transformation
-
Agrobacterium

mediated and
particle gun mediated, secondary plant metabolites
and application of plant biotechnology in crop improvement.


SECTION
-
C

7.

Animal cell culture; history, culture media
-
composition, preparation and
development, cell isolation, establishment and evaluation
of cell culture, culturing
of specialized cells, cryopreservation of animal cells.

8.

Animal cell lines : establishment, properties and use of cell lines, cultures of
tumor cells.

9.

Culture and scale up : monolayer culture
-
surface requirements, gas phase

requirements, capillary culture units, micro
-
carrier culture techniques for
laboratory and scale up of cultures, suspension culture scale up.

SECTION
-
D

10.

Somatic cell fusion : methods of somatic cell fusion, selection, properties of cell
hybrids and the
ir applications.

11.

Animal cloning and embryo transfer : superovulation,
in vitro

fertilization,
embryo transfer technology in animals; concepts and techniques of cloning; stem
cell culture and its applications.

12.

Industrial applications of animal cell
culture,

biotransformation.

Recommended Readings

1.

Plant tissue Culture and Molecular Biology, P.S. Srivastava, N.R. Book
Distributors, New Delhi, 1998.

2.

Genetic Transformation in Plants, R. Walden : Open University Press,
Buchingham, 1988.

3.

Animal Ce
ll Culture Technology, M. Buttler : Open University Press, Buchingam,
1987.

4.

Animal Cell Culture, R.I. Grechney : A.R. Liss Inc., New York, 1989.

5.

Plant Cell Culture, H.A. Collins, NR Book Distributors, New Delhi, 1998.

6.

Plant Tissue Culture
-
Theory
and Practise, S.S. Bhojwani and M.K. Razdan,
Prentice Elservier, London, 1983.

7.

Plant Tissue culture methods
-

Applications in Agriculture, J. Reinart and Y.P.S.
Bajaj Springer
-

Verlag, Berlin, 1989.

8.

Plant Cell, Tissue and Organ Culture, J. Reinart a
nd Y.P.S. Bajaj Narosa
Publication House, New Delhi, 1989.

9.

Plant Cell Culture, A. Stafford and G. Warren Open University Press,
Buchingham, 1990.

10.

Principles of Plant Biotechnology, H. Maitell, J. Mathew and R.A. Mackee,
Blackwell Scientific Publish
ers, Oxford, 1985.

11.

Plant Cell Culture Technology, M.M. Yoeman Blackwell Scientific Publication,
Oxford, 1986.

12.

Plant Cell and tissue culture a Laboratory mannual, J. Reinart and M.M. Yoeman
Narosa Publishing House, New Delhi, 1982.

Practical Paper
VII



Pertains to:


Theory paper XIII
-

Bioinformatics and Computational Biology

Theory paper


XIV: Tissue and cell culture technology


Max. Marks 100




Practical time : 60 hrs

Time : 4 hours


1)

Lymphocyte and monolayer culture technique, viability testi
ng and
subculturing.

2)

Animal cell quantitation.

3)

Growth curve of mammallian cell li
ne in culture and
determination
of cell doubting time.

4)

Orientation to a tissue culture facility.

5)

Preparation of medium

6)

Callus initiation using an explant

7)

Callus subculturing f
rom an estabilished callus

8)

Micropropagation of provided plant material.

9)

Introduction to hardening and biological hardening.

10)

Accession of molecular databases. ENTREZ, SRS.

11)

Flat file format of GENBANK and EMBL.

12)

Describe databases that can be used to access t
ext
information about human diseases.

13)

Compare the use of Entrez and ExPasy to retrieve
information about a protein sequence.

14)

Perform pairwise alignments of the proteins using PAM 30,
Pam 70 and PAM 250 matrices.

15)

Use of windows and Linux for protein softwar
es.

16)

Compare sequence
and taxonomy
information from BLAST,
PSI
-
BLAST, PHI
-
BLAST
,

eTBLAST
.

17)

Microarray/chip analysis:

18)

Creation of molecules and calculation of energy
minimization, torsion angles, band distance, bond angle,
Ramchandran plot.

19)

Prediction of Sec
ondary structure.

20)

Perform Molecular modeling of given protein structure (3D)
database using various softwares.

21)

Molecular docking: Download protein sequences and perform
docking of a substrate /inhibitor.

22)

Glucocorticoid receptors/ zinc fingers binding to D
NA

23)

Identifying the ORF using EMBOSS.

24)