TFC_Ch09b_Lect - Biology Alive

noisymaniacalBiotechnology

Feb 20, 2013 (3 years and 10 months ago)

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Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

PowerPoint
®

Lecture Slide Presentation prepared by Christine L. Case

M I C R O B I O L O G Y

a n i n t r o d u c t i o n

ninth edition

TORTORA



FUNKE



CASE

Part B

9

Biotechnology and
Recombinant

DNA

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Complementary DNA

Figure 9.9

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Recombinant DNA

Figure 9.11 (1 of 2)

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Figure 9.11, steps 3

5

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Recombinant DNA

Figure 9.11 (2 of 2)

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Making a Gene Product

Figure 9.12 (1 of 2)

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

E. coli


Used because it is easily grown and its genomics are
known


Need to eliminate
endotoxin

from products


Cells must be
lysed

to get product

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Therapeutic Applications


Subunit vaccines


Nonpathogenic viruses
carrying genes for pathogen's
antigens as
vaccines


Gene therapy
to replace defective or missing genes

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

RNA Interference (RNAi)

Figure 9.14

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Scientific Applications


Understanding of
DNA


Sequencing
organisms' genomes


DNA fingerprinting
for identification

Figure 9.17

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Southern Blotting

Figure 9.16, steps 1

2

Used for Genetic Screening

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Southern Blotting

Figure 9.16, steps 3

4

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Southern Blotting

Figure 9.16, steps 5

6

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Agricultural Applications

Table 9.2

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Figure 9.19

Result of
Agrobacterium tumefaciens

infection

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings

Genetic Modification Using
Agrobacterium

Figure 9.20