Genetic Engineering - People Server at UNCW

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Dec 14, 2012 (4 years and 10 months ago)

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Genetic Engineering




Biotechnology


Molecular Cloning


Recombinant DNA

HISTORY

Herb Boyer

EcoR1

Stanley Cohen

Competent Cells

Plasmids

Stanley Falkow

Asilomar Conference 1975

Voluntary Band of Some Recombinant Research

NIH
-
RAC formed as a result

Isolate Gene


Fragmented Total
DNA



Use mRNA and
Make DNA with
Reverse
Transcriptase

Splice into Cloning Vehicle


Plasmids


Viruses


Use Restriction
Endonuclease to
create



Sticking Ends


Rejoin Molecule with


Ligase

Inserting into Host



Transformation


Detection of
Successful Clone


DNA Library


Shotgun Cloning

Cloning Vector


Plasmid Cloning
Vectors


Single Cleavage
Site


High copy Numbers


Insertion Inactivation

Virus Vectors



Lambda Phage
Vector


Stable Packaging


Cosmids

Other Vectors


Expression Vectors


Secretion Vectors


Shuttle Vectors


Bacteriophage M13


DNA Sequencing


Yeast Artificial
Chromosome


Human Genome Project

Hosts



Cloning Vector
Hosts


E. coli


Endotoxins


Yeast

Detection of Clones




Antibodies


Nucleic Acid Probes


Synthetic DNA

Production of Recombinant
Protein


Transcription of
Cloned Gene


Promoter



Translation of
Cloned Gene


Ribosome Binding
Site

Polymerase Chain Reaction



Kary Mullins


PCR


DNA Amplification


TAQ Enzyme


Primer

PCR

Site Directed
Mutagenesis

Knockout
Mutation

Applications of Genetic
Engineering



Human Insulin



Interferon



Hepatitis Vaccine

Vaccines


Subunit Vaccines


Hepatitis Vaccine


Recombinant Vaccines


Polyvalent Vaccines


DNA Vaccines


Edible Vaccines

Other Products


Tissue Plasminogen
Activator


Rennin


Ti Plasmid


Bacillus
thuringiensis



BT


Roundup Resistant


Favosavr Tomato

More Possibilities


Ice Minus Bacteria


PCB Degrading
Bacteria


Luminescent Reporter
Gene


Transgenic Animal


Human Gene Therapy

DNA Chips
-

Microarrays

Actual DNA Chip

Genetic Eng. Word List

Biotechnology

Molecular Cloning

Recombinant DNA

Isolate Gene


mRNA,

Reverse
Transcriptase


Splice into Cloning
Vehicle
-


Plasmids, Viruses


Restriction
Endonucleases


Sticky Ends


Ligase

Insert into Host

Detect Successful
Clone


DNA Library

Shotgun Cloning

Plasmid Cloning
Vectors

Single Cleavage
Sites



Genetic Eng. Word List 2

High Copy
numbers

Insertion
Inactivation

Lambda Phage
Vector

Stable
Packaging

Cosmids


Other Vectors

Expression Vectors

Secretion Vectors

Shuttle Vectors

Bacteriophage M
13

DNA Sequencing

Yeast Artificial
Chromosomes


Human Genome
Project

Cloning Vector
Hosts

E. coli

Yeast

Detection of
Clones

Antibody


Genetic Eng. Word List 3

Nucleic Acid Probes

Transcription of
Cloned Gene

Promoter

Translation of
Cloned Gene

Ribosome Binding
Site

Synthetic DNA

Probes


Polymerase chain
Reaction



PCR

DNA Amplification

TAQ Enzyme

Primers

Site Directed
Mutagenesis

Knockout Mutations


Applications of
Genetic


Engineering

Human Insulin

Interferon

Hepatitis Vaccine

Recombinant
Vaccine

Subunit Vaccines


Genetic Eng. Word List 4

Polyvalent Vaccines

DNA Vaccines

Tissue Plasminogen
Activator

Rennin

Ti Plasmid

Bacillus thuringiensis


Roundup Resistance

Favosavr Tomato

Ice Minus Bacteria

PCB Degrading
Bacteria

Luminescent
Reporter Gene

Transgenic Animals


Edible Vaccines

Human Gene
Therapy

DNA Chips


Microarrays