Genetic Engineering
•
Biotechnology
•
Molecular Cloning
•
Recombinant DNA
HISTORY
Herb Boyer
EcoR1
Stanley Cohen
Competent Cells
Plasmids
Stanley Falkow
Asilomar Conference 1975
Voluntary Band of Some Recombinant Research
NIH
-
RAC formed as a result
Isolate Gene
•
Fragmented Total
DNA
•
Use mRNA and
Make DNA with
Reverse
Transcriptase
Splice into Cloning Vehicle
•
Plasmids
•
Viruses
•
Use Restriction
Endonuclease to
create
–
•
Sticking Ends
•
Rejoin Molecule with
•
Ligase
Inserting into Host
•
Transformation
•
Detection of
Successful Clone
•
DNA Library
•
Shotgun Cloning
Cloning Vector
•
Plasmid Cloning
Vectors
•
Single Cleavage
Site
•
High copy Numbers
•
Insertion Inactivation
Virus Vectors
•
Lambda Phage
Vector
•
Stable Packaging
•
Cosmids
Other Vectors
•
Expression Vectors
•
Secretion Vectors
•
Shuttle Vectors
•
Bacteriophage M13
•
DNA Sequencing
•
Yeast Artificial
Chromosome
•
Human Genome Project
Hosts
•
Cloning Vector
Hosts
•
E. coli
•
Endotoxins
•
Yeast
Detection of Clones
•
Antibodies
•
Nucleic Acid Probes
•
Synthetic DNA
Production of Recombinant
Protein
•
Transcription of
Cloned Gene
•
Promoter
•
Translation of
Cloned Gene
•
Ribosome Binding
Site
Polymerase Chain Reaction
•
Kary Mullins
•
PCR
•
DNA Amplification
•
TAQ Enzyme
•
Primer
PCR
Site Directed
Mutagenesis
Knockout
Mutation
Applications of Genetic
Engineering
•
Human Insulin
•
Interferon
•
Hepatitis Vaccine
Vaccines
•
Subunit Vaccines
•
Hepatitis Vaccine
•
Recombinant Vaccines
•
Polyvalent Vaccines
•
DNA Vaccines
•
Edible Vaccines
Other Products
•
Tissue Plasminogen
Activator
•
Rennin
•
Ti Plasmid
•
Bacillus
thuringiensis
–
BT
•
Roundup Resistant
•
Favosavr Tomato
More Possibilities
•
Ice Minus Bacteria
•
PCB Degrading
Bacteria
•
Luminescent Reporter
Gene
•
Transgenic Animal
•
Human Gene Therapy
DNA Chips
-
Microarrays
Actual DNA Chip
Genetic Eng. Word List
Biotechnology
Molecular Cloning
Recombinant DNA
Isolate Gene
mRNA,
Reverse
Transcriptase
Splice into Cloning
Vehicle
-
Plasmids, Viruses
Restriction
Endonucleases
Sticky Ends
Ligase
Insert into Host
Detect Successful
Clone
DNA Library
Shotgun Cloning
Plasmid Cloning
Vectors
Single Cleavage
Sites
Genetic Eng. Word List 2
High Copy
numbers
Insertion
Inactivation
Lambda Phage
Vector
Stable
Packaging
Cosmids
Other Vectors
Expression Vectors
Secretion Vectors
Shuttle Vectors
Bacteriophage M
13
DNA Sequencing
Yeast Artificial
Chromosomes
Human Genome
Project
Cloning Vector
Hosts
E. coli
Yeast
Detection of
Clones
Antibody
Genetic Eng. Word List 3
Nucleic Acid Probes
Transcription of
Cloned Gene
Promoter
Translation of
Cloned Gene
Ribosome Binding
Site
Synthetic DNA
Probes
Polymerase chain
Reaction
–
PCR
DNA Amplification
TAQ Enzyme
Primers
Site Directed
Mutagenesis
Knockout Mutations
Applications of
Genetic
Engineering
Human Insulin
Interferon
Hepatitis Vaccine
Recombinant
Vaccine
Subunit Vaccines
Genetic Eng. Word List 4
Polyvalent Vaccines
DNA Vaccines
Tissue Plasminogen
Activator
Rennin
Ti Plasmid
Bacillus thuringiensis
Roundup Resistance
Favosavr Tomato
Ice Minus Bacteria
PCB Degrading
Bacteria
Luminescent
Reporter Gene
Transgenic Animals
Edible Vaccines
Human Gene
Therapy
DNA Chips
–
Microarrays
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