Introduction to Genetic Engineering

mustardnimbleBiotechnology

Dec 11, 2012 (4 years and 8 months ago)

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Course name
Introduction to genetic engineering
Course code ENG.H.016
Department Department of Animal Physiology and Endocrinology
Faculty Faculty of Animal Sciences
Subject
supervisor/Lecturer
Assoc. prof. Andrzej Sechman, Ph.D.
General information
Teaching period semester/winter/summer
ECTS credit 2
Lectures total 15
Lab practicals ―
Objective and general
description
During the course a basic knowledge concerning animal genetic
engineering is provided.
Lectures
7 x 2 hours
plus 1 hour for the
exam
1) Structure and properties of nucleic acids;
2) Regulation of gene expression (enzymes modifying DNA and
RNA);
3) Restriction enzymes and their application;
4) Methods of DNA recombination;
5) Measurement of gene expression in animals (methods:
Southern, northern, western blot);
6) PCR and RT-PCR methods and their application in animal
physiology and endocrinology;
7) Transgenic animals.

Lab practicals


Literature
1. Molecular cloning: a laboratory manual (Sec. Ed.), J. Sambrook,
E.F. Fritch i T. Maniatis, J. Cold Spring Harbor Laboratory
Press, Cold Spring Harbor, 1989.
2. “Recombinant DNA”, James Watson i inn., Scientific American
Books, New York, 1992.
3.„Genes V”, Benjamin Lewin, Oxford University Press, Oxford
New York Tokyo, 1994