B.Sc. (Industrial) Microbiology) Part I & II - Punjabi University Patiala

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Feb 12, 2013 (4 years and 6 months ago)

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Syllabus

For

B
.Sc.
INDUSTRIAL
MICROBIOLOGY

PART
-

I

(
ANNUAL SYSTEM
)

20
11, 2012 and 2013
Examination
s

P
u
njabi University

Patiala


B.Sc.
-

Industrial Microbiology

Course and Subjects to be thought during 3 Years



B.Sc.
-
I










MARKS




Paper I.

Punjabi








150


Paper II . Chemistry A, B & C






300


Paper III. Cell Biology A & B






200


Paper IV. Fundamentals of Microbiology A & B





200


B.Sc
-

II

Paper I. English








150

Paper II. Chemistry A, B & C






300

Paper

III. Fundamentals of Biochemistry A & B




200

Paper IV. Microbial Physiology, Metabolism &




200


Microbial Genetics A & B


B.Sc.
-

III


Paper I.

Chemistry I, II, III






300

Paper II. Food & Industrial Microbiology I & II



200

Pap
er III. Environmental Microbiology &




200


Microbial Technology I & II

Paper IV. Computer, Bio
-
statistics and Bio
-
informatics.




50

Paper V. Entrepreneurship Development.





50



















PAPER III: CELL BIOLOGY


Paper III
-
A

M. Marks
: 75







Lecturer to be delivered: 80

Pass Marks: 35%






Time allowed: 3 hours


INSTRUCTIONS FOR THE PAPER SETTER & CANDIDATES



The question paper will consist of five sections: A, B, C, D and E. Sections A, B,
C and D will have two questions from the

respective sections o
f the syllabus and will
carry 12

marks ea
ch. Section E will consist of 12
-
15

short
-
answer type questions

(2
-
3
marks each)

which will cover the entire syllabus uniformly and will carry
27

marks in all.

Candidates are required to attem
pt one question each from Sections A, B, C and D
of the question paper and the entire Section E.


SECTION
-
A

1.

Cell as a basic unit of Living systems : Cell Theory and Cell Principle

2.

Broad classification of cell types : PPLO’s bacteria, plant and anim
al

cells; Cell, tissue, organ and organism as different level of organization of
otherwise genetically similar cells; Types of cells in organisms.

3.

Evolution: Pre
-
cell evolution, artificial creation of “cells”, Structure of eukaryotic
and prokaryotic ce
ll.

SECTION
-
B

4.

Ultra structure and function of cell Organelles

(a) Endoplasmic reticulum: Structure, types, biogenesis and functions of RER and
SER. General Account on Microsomes and Signal Hypothesis.

(b) Cytoplasm: Cytoskeleton, Endo
-
membrane
System and membrane organelles.

(c) Golgi Bodies: Morphology of golgi complex.,Cytochemistry of golgi complex.
Function of Golgi Complex.
-

Endocytosis, Recycling for secretion process.

SECTION
-
C

5.

Ultra structure and function of cell Organelles

(a) R
ibosomes: Ultra structure of r
-
RNA, m
-
RNA and t
-
RNA., Prokaryotic and
Eukaryotic Ribosome, r
-
RNA biogenesis. Function of ribosomes.


(b) Mitochondria: Ultra structure and function of mitochondria, Biogenesis of
mitochondria, Bioenergetics
-

Role of mitochon
dria in Kreb’s cycle, glycolysis
cycle Respiratory chain complexes, Electron transport system, mitochondrial
ATPase proton pump complex.


(c) Chloroplast: Ultra structure, morphology of chloroplast. Function and
Biogenesis of chloroplast.



(d) Lysosomes
and peroxisomes: Major characteristics of lysosomes. Functions
-
Intracellular digestion, endocytosis. Morphology of peroxisomes and
microperoxisomes. Biogenesis of peroxiosomes.



SECTION
-
D

6.

Ultra structure and function of cell organelles.

(a) Cytoskel
etal Structures: Microfilaments, intermediate filaments and
Microtubules, Microtubular organelles
-

Ultra structure and function of cilia
flagella and centrioles .


(b) Nucleus: Ultra structure of Nucleus, nuclear envelope, euchromatin and
hetrochromatin
, microfilaments. Brief account of chromosome structure.

(c) Biological Membrane: Ultra structure of plasma membrane and cell wall.


Transportation across the membrane. Model membranes and liposomes.

Functions of biological membranes.















Paper III
-
B

M. Marks: 75







Lecturer to be delivered: 80

Pass Marks: 35%






Time allowed: 3 hours


INSTRUCTIONS FOR THE PAPER SETTER & CANDIDATES



The question paper will consist of five sections: A, B, C, D and E. Sections A, B,
C and D wil
l have two questions from the respective sections of the syllabus and will
carry 1
2

marks each. Section E will consist of 1
2
-
15

short
-
answer type questions

(2
-
3
marks each)

which will cover the entire syllabus uniformly and will carry 15 marks in all.

Can
didates are required to attempt one question each from Sections A, B, C and D
of the question paper and the entire Section E.


SECTION
-
A

1.

Biochemical composition of cells Cellular pool, water, mineral salts, proteins,
carbohydrates, lipids and nucleic
acids.

2.

Ecological amplitude of cell in high altitude, sediments arctic, hot spring, acidic
brackish and fresh water environment.

SECTION
-
B

3.

Cell Division and Cell Cycle: Cell cycle and general description of mitosis and
meiosis. Binary fission, Amitos
is, Molecular organization of mitotic spindle
apparatus.

4.

Cellular Interactions: Cell recognition and cell coat, Differentiation of cell
membrane, Cell surface of cancer cells and altered coupling of cancer cells, Inter
cellular communication and gap jun
ctions.

5.

Cell Locomotion: Cytoplasmic streaming in plant cells, amoeboid motion, Cilial
and flagellar movements

SECTION
-
C

6.

Cell Senescence and Death: Apoptosis and necrosis

7.

Cell differentiation: General characteristics of cell differentiation in pl
ants and
animals



SECTION
-
D

8.

Cellular and Molecular Neurobiology: General organization of nerve fibres
Functions of nerve fibers. Structure of synapse and synaptic transmission.
Synaptic vesicles and synaptic receptor.


9.

Cellular and Molecular Biology

of Muscles: Structure of striated muscle fibre,
Molecular organization of contractile system, Regulation and energetic of
contraction, Sliding mechanism of muscle contraction, Excitation
-
contraction
coupling.

BOOKS RECOMMENDED

1.

Lodish, I, et al .1995.

Molecular Cell Biology

Third ed., Scientific American


Book Inc.

2.

Geoffrey 2000 .
The Cell: Molecular Approach

2
nd

Ed. ASM Press.

3.

De Robertis 1991.
Cell and Molecular Biology
, Saurders, Philadelphia.

4.

C.P. Powaar 2003.
Cell Biology

(
Indian ed.



PRACTICALS

NOTE
: Candidate will be required to submit their original notebook of lab work
initiated with date by their teacher at the time of practical examination.



Students must be taken for excursion to industries, laboratories to have
an
exposure to daily life use of the subject.



Practical examination shall be held in a single morning session from 9:00 a.m. to
1:00 p.m.



Practical examination is to be conducted by the two external examiners.

Guidelines pattern for the conduct of pra
ctical examination.

Max. Marks: 50





Time Allowed: 8 Periods/week

Internal Assessment:



10 marks

Practical Records:



5 marks

Viva Voice:




5 marks

Industrial visits excursion trips:

5 marks

Practical exercises :



25 marks.


LIST OF PRACTICALS

1.

Microscopy
-


Principles of compound, phase contrast
and

electron microscopy.

Use and care
of light or compound microscope.

2. Study of Cells (1) Prokaryotic cells
Lactobacillus, Escherichia coli
, blue green algae.




(2) Eukaryotic cells
-

Testicul
ar material for spermatogenesis.

3. Microtomy

: Instruments introduction, use, care and section cutting.

4. Preparation of permanent slides
: Section cutting of plant tissues and staining


5. Study of permanent slides of various tissues: gut region, Li
ver, Lung, Spleen, Kidney,
pancreas, Testis. Ovary, Tongue, Skin etc.

6. Cytochemical techniques to study carbohydrates, nucleic acids and proteins.

7. Study of electron micrographs of various cell organelles:

Plasma Membrane, Mitochondria, Golgi Comple
x, Lysosomes, Endoplasmic
Recticulum (SER+ RER), Cilia, centriole, inclusion, like glycogen, lipids etc.


BOOKS RECOMMENDED

Celis J.E. (1998)

: Cel Biology
-

A laboratory handbook U.K.



















PAPER IV: FUNDAMENTALS OF MICROBIOLOGY

PAPER

IV
-
A

M.
Marks: 75







Lecturer to be delivered: 80

Pass Marks: 35%






Time allowed: 3 hours


INSTRUCTIONS FOR THE PAPER SETTER
and

CANDIDATES



The question paper will consist of five sections: A, B, C, D and E. Sections A, B,
C and D will have two questions f
rom the respective sections of the syllabus and will
carry 1
2

marks each. Section E will consist of 1
2
-
15

short
-
answer type questions

92
-
3
marks each)

which will cover the entire syllabus uniformly and will carry
27

marks in all.

Candidates are required t
o attempt one question each from Sections A, B, C and D
of the question paper and the entire Section E.


SECTION
-
A

1.

Introduction: Historical development, application of microbiology and its


relevance to biotechnology, status of microbiology in India.

2.

Microscope and Microscopy: Brief account of structure, principle and
applications of bright field, fluorescence and electron scanning microscopes,
Fixation and fixatives, positive,
negative, acid fast and Gram staining techniques.

SECTION
-
B

3.

Classification

: Microbiological diversity, concept of microbial species,
classification of micro
-
organisms, Microbial taxonomy
, General methods of
characterization. Classification and identification of microorganisms. Bacterial
nomenclature, a brief account of Bergey’s system of bacterial classification.


SECTION
-
C

4.

Prokaryotes: A general account on characteristics, structure
reproduction
and functions to cell organelles of


Bacteria, Archaebacteria, Cyanobacteria,
Actinomycetes, Mycoplasma

5.

Eukaryotes: A general accountt of characteristics, morpholog
y, nutrition,
fine structure, economic importance and function of Molds, Slime molds, yeast ,
Algae, Protozoa

SECTION
-
D

6.

Viruses
:
General account of viruses, detailed structure a
nd
reproduction of

Bacteriophages, Cyanophages, Plant Viruses and Animal Viruses

7.

Microorganisms as Geochemical

Agents: Cycles of matter (Nitrogen, Carbon,
Water, Oxygen, Sulphur and Phosphorus) and other interactions of Microbes.



PAPER

IV
-
B

M. Marks:
75







Lecturer to be delivered: 80

Pass Marks: 35%






Time allowed: 3 hours


INSTRUCTIONS FOR THE PAPER SETTER
and

CANDIDATES



The question paper will consist of five sections: A, B, C, D and E. Sections A, B,
C and D will have two questions from the

respective sections of the syllabus and will
carry 15 marks each. Section E will consist of 10 short
-
answer type questions which will
cover the entire syllabus uniformly and will carry 15 marks in all.

Candidates are required to attempt one question each

from Sections A, B, C and D
of the question paper and the entire Section E.


SECTION
-
A

1.

Microbial Nutrition: Requirement of nutrients for microbes and nutritional

categories amon
g micro
-
organisms. Choice of media and conditions of
incubation.

2.

Culture Characteristics: Colony and broth culture characteristics. Maintenance
and preservation of pure cultures.

SECTION
-
B

3.

Pure Culture: Pure culture techniques, preparation of differ
ent types of culture
media, selective or enrichment media. Methods of culturing aerobic and anaerobic
bacteria. Culture characteristics, smearing and staining, sterilization techniques.

4.

Microbial Growth: Growth curve, mathematical expression of growth,

methods

of measurements of growth. Factors affecting growth in continuous and batch
cultures, detail of synchronous and diauxic growth.

5.

Strain improvement: Methods of improvement and stability of biotechnologically


important cultures

SEC
TION
-
C

6.

Control of Microorganisms: Control of microorganisms by physical and chemical
agents, patterns of microbial death, factors affecting effectiveness of antimicrobial
agent’s activity

7.

Antimicrobial Chemotherapy: development of chemotherapy, gene
ral
characteristics of antimicrobial drugs and mechanism of action of anti microbial
agents. Origin of drug resistance and its transmission in microorganisms.


SECTION
-
D

8.

Biological Nitrogen Fixation

: Microbiology of symbiotic and non
-
symbiotic
nitrogen

fixation. Symbiotic Nitrogen fixing system, process of root nodule
formation. Metabolism of free living and symbiotic micro
-
organisms. Nitrogen
fixation mechanism with structure and function of nitrogenase.

9.

Food Poisoning: Microbiology of food intoxica
tions. Epidemiology of food
intoxications. Food borne infections
-
mode of transmission and their control

(Clostridium, Salmonella, Shigella, E
.
coli,

Staphylococcus aureus, Aflatoxin and
Algal toxins)

10.

Human microflora: Microbial flora of healthy human h
ost
-
origin, distribution and

occurrence of normal flora, Germ free and antibiotic life .Effect of antimicrobial
agents.

BOOKS RECOMMENDED

1.

Davis, B.D. Bullbecco, R., Eisen and Ginsberg H.S. 1990.
Microbiology

: 4th Ed.
Harper and Row, Publishers, Singa
pore

2.

Tortora, G.J. Funke,

B.R. and Case, C.L. 1994.
Microbiology: An Introduction

:
5th Ed. The Bejnamin cummings Publishing Company, Inc.

3.

Stainer, R.Y. 1995.
General Microbiology
,. Macmillan Press,London.

4.

Pelezar, M.T. 1995.
Microbiology,

Tata McGraw Hill Publishing, New Delhi.

5.

Schlegel, H.G. 1995.
General Microbiology

7th Ed., Cambridge Univ. Press.

6.

Prescott and Dunn 1999.
Industrial Microbiology

4th Ed., Cambridge Uni. press.

7.

Purobit, S.S. 2000.
Microbiology
: Fundamentals and A
pplication (6
th

ed.)


Agrobios, Jodhpur(India).

8.

Postagate, J. 2000. Microbes and MAN 4th Ed., Cambridge Uni. Press.



PARACTICALS

NOTE:


Candidates will be required to submit their original notebook of lab work
initiated with date by thei
r teacher at the time of practical examination.


Students must be taken for excursion to industries, laboratories to have an
exposure to daily life use of the subject.


Practical examination shall be held in a single morning session from 9:00 a.m. to
1:0
0 p.m.



Practical examination is to be conducted by the two external examiners.

Guidelines pattern for the conduct of practical examination.

Max. Marks: 50





Time Allowed: 8 Periods/week

Internal Assessment:



10 marks

Practical Records:



5 marks

Viva Voice:




5 marks

Industrial visits excursion trips:

5 marks

Practical exercises :



25 marks.


LIST OF PRACTICALS

1.

Simple, negative, capsule, acid fast and gram staining of microorganisms.

2.

Morphological and Biochemical characterization of mi
croorganisms.

3.

Isolation of microorganisms by pour plate and streak plate method.

4.

Strain improvement by physical and chemical mutagenesis.

5.

Determination of coliform bacteria in water.

6.

Determination of viability of microorganism.

7.

Microscop
e measurement of size of microorganism.

8.

Hanging drop preparation to check the mobility/Brownian movements of
microorganisms.

9.

Microbial growth curve and Measurement of microbial growth.

10.

Factors affecting the growth of Microorganisms.

NOTE:

The a
wards in Internal Assessment for each practical paper must reach controller
of Examination, Punjabi University, Patiala within in one week of the start of the Theory
Examination.


Syllabus

For

B
.Sc.
INDUSTRIAL
MICROBIOLOGY

PART
-

II

(
ANNUAL SYSTEM
)

20
11,
2012 and 2013
Examination
s


P
u
njabi University

Patiala

B.Sc. II (Industrial Micro Biology)




PAPER III:
-

FUNDAMENTAL OF BIOCHEMISTRY


Paper III
-
A

M. Marks: 75







Lecturer to be delivered: 80

Pass Marks: 35%






Time allowed: 3 hours


INSTRUCTIONS FO
R THE PAPER SETTER
and

CANDIDATES



The question paper will consist of five sections: A, B, C, D and E. Sections A, B,
C and D will have two questions from the respective sections of the syllabus and will
carry 1
2

marks each. Section E will consist of 1
2
-
1
5

short
-
answer type questions
(2
-
3
marks each)
which will cover the entire syllabus uniformly and will carry
27

marks in all.

Candidates are required to attempt one question each from Sections A, B, C and D
of the question paper and the entire Section E.




SECTION
-
A

1. Introduction to Biochemistry: Water as a biological solvent, dissociation of water, pH,
buffer solutions, Handerson
-
Hasselbalch equation.

2. Amino acids: amino acids titration curves, physical and chemical properties of amino
acids, p
eptide bonds, rigid and planar nature of a peptide bond, Ramachandran Plot,
folding of peptide chains into regular repeating structure (helix, pleated sheets).β. turns in
polypeptides, amino acid sequencing of poly peptides.


SECTION
-
B

3. Proteins: Levels

of structure in protein architecture, Forces stabilizing structure and
shape of proteins, native proteins folding and design.

4. Carbohydrates: Fisch and Hawarth structures of Carbohydrates, stereoisomerism and
mutarotation, anomeric forms of monosacchar
ides, reactions of monosaccharides,
characteristics of the aldehyde, ketones and hydroxyl groups, glycosidic bonds, amylose,
amylopectin, starch, cellulose, chitin, pectins glycosaminoglycans
(mucopolysaccharides).

SECTION
-
C

5. Structure and functions o
f lipids, fatty acids, triacylglycerols, glycerophospholipids,
sphingomyelins, lipoproteins, Liposomes, biological membranes and micelles.

6 Nucleic Acids: Structure and properties of purine and pyrimidines bases. Nucleosides
and nucleotides, biologic
al functions of DNA and RNA species. Double helical model of
DNA and forces responsible for it. Short hand representation of nucleic acid back bone,
denaturation of DNA, methods for isolation and purification of nucleic acids.


SECTION
-

D

7. Introduction
to enzymes and coenzymes, units of enzyme activity, enzyme
nomenclature and classification. Enzyme kinetics, effect of substrate concentration on
Michaelis
-

Menten equation, determination of Km and its significance, effect of pH and
temperature on rates o
f enzymes catalyzed reaction.

8. Enzyme inhibitors and their importance, chemical methods of active site studies,
Introduction of multi substrate enzymes, allosteric enzyme and enzyme regulation, iso
enzymes, enzyme immobilization.







Paper: III
-
B





M. Marks: 75







Lecturer to be delivered: 80

Pass Marks: 35%






Time allowed: 3 hours


INSTRUCTIONS FOR THE PAPER SETTER
and

CANDIDATES



The question paper will consist of five sections: A, B, C, D and E. Sections A, B,
C and D will have two questions from the respective sections of the syllabus and will
carry 1
2

marks each. Section E will consist of 1
2
-
15

short
-
answer type questions
(2
-
3
marks each)
which will cover the entire syllabus uniformly and will carry
27
marks i
n all.

Candidates are required to attempt one question each from Sections A, B, C and D
of the question paper and the entire Section E.


SECTION


A

1. Bioenergetics: Biological system and general Laws of thermodynamics , concept of
entropy , high ener
gy bonds , biological oxidation and redox potentials, mitochondrial
electron transport chain and oxidative phosphorylation.

2. Metabolic pathways: Basic concept and design, glycolysis, TCA cycle, Pentose
phosphate pathway, gluconeogenesis and glycogen met
abolism.

SECTION
-

B

3 β
-
Oxidation of fatty acids, formation of ketone bodies, Biosynthesis and catabolism of
triglycerides, phosphoglycerides and sphingolipids, synthesis of cholesterol and fatty
acids

4. Deamination, Transamination, decarboxylation reac
tion, hormones and vitamins, Urea
cycle, Biosynthesis and degradation of purines and pyrimidines.

SECTION
-
C

5. Protein Metabolism: Introduction, structure and properties of amino acid, their types,
peptide bonding, primary, secondary and tertiary struc
ture of proteins, concepts of
polypeptides, Brief account of protein synthesis.

6. Nucleotide Metabolism: synthesis of purine and pyrimidine and their degradation

SECTION
-
D

7. Spectroscopic techniques:
-

Beer’s Lambert Law, U .V. Visible spectrophotometer
y,
IR spectroscopy and spectrofluorimetry; principal and biological applications.

8. ORD and CD spectroscopy, NMR and mass spectrometry: principal and Biological
application.

9. Electrophoretic techniques: Principles and applications of Electrophoretic t
echniques
in purification and characterization of biomolecules, isoelectric focusing, and
immunoelectrophoresis, SDS
-
PAGE and agarose gel electrophoresis



RECOMMENDED BOOKS


1.

Lehninger, Nelson and cox 2000.
Principles of Biochemistry
, 3rd Edition, 20
00:.

2.

Lubert Streyer 1995.
Biochemistry
, 4th ed.,

3.

K. Rangnathan Rao.

1986:
Text book of Biochemistry
, 3rd ed.

4.

J.L. Jain. 2000.
Fundamentals of Biochemistry
, 5th ed., S. Chand and Co., New
Delhi.

5 Upadhyay and Nath 2002.
Biophysical C
hemistry
-

Principles and Techniques

, 3rd
ed. Himalaya Publishing House.

6.

Raymond Chang 1989.
Physical Chemistry with application to Biological
systems
:, 2nd Ed. MacMillan Publishing Co. 2 No. New York


PRACTICALS

NOTE:


Candidates will be required to
submit their original notebook of lab work
initiated with date by their teacher at the time of practical examination.


Students must be taken for excursion to industries, laboratories to have an
exposure to daily life use of the subject.


Practical exami
nation shall be held in a single morning session from 9:00 a.m. to
1:00 p.m.



Practical examination is to be conducted by the two external examiners.

Guidelines pattern for the conduct of practical examination.

Max. Marks: 50





Time Allowed: 8 Period
s/week

Internal Assessment:



10 marks

Practical Records:



5 marks

Viva Voice:




5 marks

Industrial visits excursion trips:

5 marks

Practical exercises :



25 marks.



Subject: Fundamental Biochemistry

1.

Preparation of solutions and buffers.

2.

Protein
estimation by Lowry’s method and pyne’s method.

3.

Estimation of carbohydrates (glucose, fructose, lactose)

4.

Qualitative estimation of Lipids in given sample.

5.

To study enzyme activity and enzyme kinetics

6.

TLC of amino acids.

7.

SDS
-

PAGE for protein analysis

8.

Agaro
se gel electrophoresis for nucleic acids.

9.

Isolation of DNA and RNA

10.

Estimation of titrable acidity of given food sample

11.

To Determine saponification and iodine value of given fat/ oil sample

12.

Immobilization of enzyme/ bacterial cells in calcium
-

alginate bead
s



Books Recommended:


DT Plummer 1988.
An introduction to practical Biochemistry

(3
rd
) Tata Mc Graw Hill


PAPER IV:
-

MICROBIAL PHYSIOLOGY, METABOLISM AND GENETICS


Paper IV
-
A

M. Marks: 75







Lecturer to be delivered: 80

Pass Marks: 35%






Time al
lowed: 3 hours


INSTRUCTIONS FOR THE PAPER SETTER
and

CANDIDATES



The question paper will consist of five sections: A, B, C, D and E. Sections A, B,
C and D will have two questions from the respective sections of the syllabus and will
carry 1
2

marks each.

Section E will consist of 1
2
-
15

short
-
answer type questions

(2
-
3
marks each)

which will cover the entire syllabus uniformly and will carry
27

marks in all.

Candidates are required to attempt one question each from Sections A, B, C and D
of the question p
aper and the entire Section E.


Section


A


Biochemical components of microbial cell


structure and functions of organelles of
microbial cell, Role of cell wall and cell membrane in form and functions of microbial
cells, Transportation


passive diffus
ion, facilitated diffusion, active transport and group
translocation.


Section


B


Mode of nutrition, classification of microorganisms, growth factors, growth of
microorganisms: phase of growth, parameter for measuring growth; Resting forms in
microorgani
sms
-

physiology of endospore formation and its generation, control of
microorganisms


Section


C

Bioenergetics:
-

Basic concepts, redox in energy pairs in energy metabolism substrate
level and oxidative phosphorylation, Components of ETC, Nitrogen Fixati
on


in brief

Regulation of Metabolism: Enzyme induction, catabolite repression, feed
-

back inhibition
and repression



Section


D


General properties of archaebacteria, salient features and difference from eubacteria

Brief account of physiology of the
rmopiles, halophiles and methanogens







Paper IV
-
B

M. Marks: 75







Lecturer to be delivered: 80

Pass Marks: 35%






Time allowed: 3 hours


INSTRUCTIONS FOR THE PAPER SETTER
and

CANDIDATES



The question paper will consist of five sections: A, B,
C, D and E. Sections A, B,
C and D will have two questions from the respective sections of the syllabus and will
carry 1
2

marks each. Section E will consist of 1
2
-
15

short
-
answer type questions

(2
-
3
marks each)

which will cover the entire syllabus uniforml
y and will carry
27

marks in all.

Candidates are required to attempt one question each from Sections A, B, C and D
of the question paper and the entire Section E.


Section


A

General account on heredity: DNA as genetic material, its structure and repli
cation, DNA
damages and repairs

Concept of RNA mRNA, tRNA and rRNA. Gene structure, genetic code, organization of
the code for transcription and translation.



Section


B


Mutations: Introduction, types of mutations, mutagenesis and mutants, spontaneous
mutation and induced mutation, frame shift mutations, mis
-
sense, non
-
sense mutation,
Reversion and suppression.


Section


C


Bacterial Genetics: Conjugation, transformation and transduction (generalized and
specialized), plasmid, fertility factor, resista
nce factor, transposable elements,


Section


D


Bacteriophage : General properties, structure, life cycle of T
4
and


-
Phage

Gene Expression and regulation: transcription, translation, operon model, tryptophane
and Lac
-

operon, and role of genetic enginee
ring in molecular biology.



Recommended Books


1.

Bainbridge BW. 1987.
Genetics of Microbes
,
Blackie, UK

2.

Friefelder D. 1987.
Microbial Genetics

Narosa Publ. New Delhi

3.


Friefelder D., Malay S.R. and Cronan J.E. 1994. Microbial Genetics. Jones and
Bartleet Pub

NY

4.


Moat, AG and Foster, JW .
Microbial Physiology

John Wiley and sons.

5.


Gottschalk.E
Microbial metabolism

Springer verlag.



PRACTICALS

NOTE:


Candidates will be required to submit their original notebook of lab work initiated with
date by their tea
cher at the time of practical examination.


Students must be taken for excursion to industries, laboratories to have an exposure to
daily life use of the subject.


Practical examination shall be held in a single morning session from 9:00 a.m. to 1:00
p.m
.



Practical examination is to be conducted by the two external examiners.


Guidelines pattern for the conduct of practical examination.

Max. Marks: 50





Time Allowed: 8 Periods/week

Internal Assessment:



10 marks

Practical Records:



5 marks

Viva

Voice:




5 marks

Industrial visits excursion trips:

5 marks

Practical exercises :



25 marks.


Subject: Microbial Physiology, metabolism and Genetics

1.

Morphological and cultural characterization of bacteria.

2.

Bacterial growth curve and measurement of b
acterial growth.

3.

IMViC Test.

4.

Enumeration of bacteria based on their temperature optima.

5.

Bacterial Motility using hanging drop technique.

6.

Estimation of total DNA in given suspension by spectroscopy.

7.

Isolation of spontaneous mutants for

antibiotic resistance.

8.

To study replica plating.

9.

To study antibiotic sensitivity profile of microbial isolates.

10.

To carry out isolation of (induced) mutants using UV.

11.

Isolation of Genomic DNA from microorganisms.