ELECTROMAGNETIC FIELD ON

murmerlastUrban and Civil

Nov 16, 2013 (3 years and 6 months ago)

72 views

EFFECTS OF AN
ELECTROMAGNETIC FIELD ON
BOAR SPERM PERMEABILITY

Alita Loveless, PGY 4

M. Sellers, L. Welch, S. Prien


Introduction



Electromagnetic fields are everywhere



Produced by alternating current



Can affect and possibly damage human tissues
by thermal effect and disruption of molecular
structure



Questionable links to various medical problems

Previous Experiments



Exposure to extremely high EMFs caused a
decrease in semen parameters



Parameters are indicative of cell function

Short-term Effects of EM Fields on
Sperm Motility in Culture
0
10
20
30
40
50
60
70
0 h
0.5 h
1 h
1.5 h
2 h
2.5 h
3 h
time
Motility (%)
Control
EMF
P < .001

Short-term Effects of EM Fields on
Sperm Forward Progress in Culture
0
0.5
1
1.5
2
2.5
3
3.5
4
0 h
0.5 h
1 h
1.5 h
2 h
2.5 h
3 h
time
Forward Progression
(1-5 scale)
Control
EMF

Questions


A way to detect signs of cell damage
before cell function was altered



Mechanism leading to decreased cell
function

Theoretically…


Long
-
term depolarization of a cell membrane
when subjected to an EMF



Shift in membrane permeability



Potential effect on osmoregulation and disruption
of cell function

Common Lab Equipment


Hood




8 mG


Centrifuge



36 mG


Incubator


Upper shelf




86 mG


Middle shelf



32 mG


Lower shelf




2 mG

Materials and Methods


Porcine semen samples


Incubated at 37 degrees Celcius with and
without exposure to an EMF


Removed samples, combined them with
fluoroscein


Examined the samples under a fluorescent
microscope to determine percentage
permeabilized

Results


Decreases in standard
semen parameters


Results



Effects on path
straightness and linearity

Results


Increased cell permeability with EMF exposure

Conclusion


Short
-
term exposure
to an EMF appears to
increase sperm
permeability and alter
cell physiology

Clinical Relevance


Assisted Reproductive
Technology labs


Environment is critical


Could basic equipment
be affecting our
success rates?

Resources and Thanks…



Thanks to Lisa Welch, Matt Sellers, and
Dr. Prien


Previous research: Dr. Naghma Farooqi


WHO EMF Project


http://www.who.int/peh
-
emf/en/