Syllabus for Comprehensive Examination - Department of ...

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Feb 12, 2013 (4 years and 4 months ago)

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DEPARTMENT OF BIOTECHNOLOGY

INDIAN INSTITUTE OF TECHNOLOGY MADRAS

CHENNAI
-
600036

SYLLABU
S FOR COMPREHENSIVE EXAMINATION



Syllabus for Biochemistry

• Chemistry of biomolecules
-

essentials of structure and classification of carbohydrates, lipids, amino
aci
ds and proteins and nucleic acids.

• Supramolecular assemblages and arrangement of cell organelles.

• Biocatalysis: Enzymes


structural aspects, cofactors, activation, catalytic mechanism
-
specific
examples. Enzyme kinetics


Michaelis
-
Menton equation, c
lasses of inhibition. Allosteric
enzymes, isoenzymes and multienzyme complexes.

• Concepts in bioenergetics:Thermodynamics considerations, bioenergetic principles.

• Metabolism: Overview, important pathways related functions and inter
-
relationships
-
glyc
olysis,
Citric acid cycle; hexose monophosphate shunt ; mitochondrial electron transport chain; oxidative
phosphorylation process; ATP synthase; oxidation of fats and amino acids; urea cycle;

• Photosynthesis: structure and function of chloroplasts, Photo
systems (I and II), Light and dark
reactions, C3 and C4 plants, Absorption of solar energy, chlorophylls and other light absorbing
pigments in nature.

• Principles and Techniques of Biochemistry: Quantitative biochemical measurements (nucleic acid,
protei
n and sugar estimations), principles of clinical biochemical analysis, Electrophoretic
techniques, Spectroscopic techniques, Chromatographic techniques, Radioisotope techniques.


Reference Reading:

1. Voet, D., Judith and Voet, G. Third edition, (2004),
Biochemistry, Wiley Publication.

2. Nelson, D.L., Lehninger, A.L. and Cox, M.M. Fourth edition, (2004) Principles of Biochemistry ,
Worth Publishers.

3. Berg, J., Tymoczko, J., Stryer, L. and Clarke, N.D. Fifth edition, (2000), W.H. Freeman
Publication.

4. Keither Wilson, and John Walker. Sixth edition (2005), Cambridge University Press.


Molecular biology

Chromatin Assembly and gene structure, Replication (Replication Enzymes), Replication


(Replication
Fork), Replication (Prokaryotic System) Replicatio
n (Eukaryotic Systems), DNA Mismatch Repair, DNA
Repair II DS Break Repair, Homologous Recombination, Homologous Recombination Enzymes, Site
-
specific Recombination, Meiotic Recombination, Transposition, Operon and regulation of gene
expression


Transcripti
on: Prokaryotic and Eukaryotic Machinery and Promoters, Transcription:
Regulation I, Transcription Regulation II, RNA Processing: Stable RNAs and RNA Editing, RNA
processing; Importance,


Localization etc.,.


RNA Processing: Mechanism, Translation: Basic M
achinery
I Translation: Basic Machinery II, Translation: Regulation.

References:

Genes by Benjamin Lewin

Molecular biology of the cell by
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith
Roberts, and Peter Walter


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Genetics


1. What is

Genetics? Heredity & Continuity of Life

2. History of Genetics
-
Landmarks

3. Chromosomes, Cell division
-
Mitosis and Meiosis

4. Presence of DNA in Chromosomes

5. Reproductive Cycles

6. Mendelian Principles
-

Law of Segregation of Genes and Chromosomes

7. Pri
nciple of Independent Assortment

8. Mendelian traits in Man

9. Interaction of Genes
-
Dominance and Multiple Alleles
-
Incomplete Dominance,
---
Over
Dominance, Co Dominance, Epistasis etc...

10. Multiple factor Inheritance
-
Qualitative
and Quantitative traits

11. Heredity & Environmental effects

12. Allelism and Pleiotropism

13. Lethal genes, Penetrance & Expressivity

14. Nature
-
Nurture problem in Man

15. Sex linked Inheritance

16. Linkage and Crossing over

17. Genetic maps of Chromosom
es

18. Chromosomal Aberrations & Cytological maps

19. Spontaneous Mutation

20. Induced Mutation

21. Genes in Populations

22. Crossing, Selfing, Inbreeding and Heterosis

23. Genetic Control of Development

27. Cytoplasm in Heredity & Environment

28. Elements

of Genetic System

29. Bacetiophage, Prokaryotic and Eukaryotic genetics

30. Organisation of Genetic material

31. Statistical Genetics

Reference texts:

3


1.
Anthony J
. F. Griffiths
,
Jeffrey H. Miller
,
David T. Suzuki
,
Richard C. Lewontin
,
William M. Gelbart

(2003) An Introduction to Genetic Analysis. W.H. Freeman publishers

2.

Hartl, D.L. and Jones, E.W. (2002) Essential Genetics. Jones and Bartlett. Monroe, W. Strickberger.
(1996) Genetics. Prentice Hall.

3.
Monroe W. Strickberger

(1985)
Genetics.
Prentice Hall.

4
. Edmund W Sinnot, LC Dunn & Theodosius Dobzhansky. (1973) Principles of Genetics. Tata McGraw
Hill.



MICROBIOLOGY

Evolution of microbes; Bacterial taxonomy; Structure
and function of prokaryotes and eukaryotes;
Growth of microbes; Metabolism of microbes; Microbial genomics; Microbes in biogeochemical cycles;
Microbial ecology; Extremophiles; Microbial interactions; Industrial microbiology; Environmental
microbiology

Ref
erence

Prescott's Microbiology by Joanne Willey, Linda Sherwood, Chris Woolverton. 8th Edition. Publisher:
McGraw
-
Hill


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DEPARTMENT OF BIOTECHNOLOGY

INDIAN INSTITUTE OF TECHNOLOGY MADRAS

CHENNAI
-
600036

SYLLABUS FOR COMPREHENSIVE EXAMINATION

D
own stream Pro
cessing:

Various down stream process steps, Fundamental principles of obtaining the product from cell cultures


intracellular vs. extra
-
cellular product

;
Cell disruption


mechanical, enzymatic, and chemical methods;
Pre
-
treatment strategies; Solid
-
liqui
d separation


filtration and centrifugation; Adsorption; Liquid
-
liquid
extraction; Membrane separation


ultrafiltration and reverse osmosis, dialysis etc; Precipitation of
proteins by different methods; Chromatograph


principles, instruments and practic
e
;
Crystallization;
Distillation; Drying.

Text Book:

Bioseperation: Downstream Processing for Biotechnology, Paul A. Belter, E.L. Cusler, Wei
-
Shou Hu,
John
-
Wiley & Sons.

Process engineering
p
rinciples:

Units and dimensions, Dimensional analysis, dimensionl
ess numbers, Fluid mechanics: Fluid definition
and classification, types of fluids, laminar and turbulent flow, Continuity equation and flow through
circular and non
-
circular conduits, settling and sedimentation. Fluid Flow: Various devices for flow
measur
ement, Pumps and their characteristics. Mechanical operations: Size reduction, mixing devices,
Sedimentation and filtration. Heat Transfer: Modes of heat transfer, Conduction, steady state and
unsteady state though walls, insulations, Convective heat trans
fer, forced and natural convection,
condensation,.

Heat Transfer Equipment: Design of Double pipe, shell and tube, evaporator and sterilizers.

Mass Transfer: Diffusion, Ficks law, interpahse mass transfer, mass transfer correlations and their
correlations.

Mass Transfer operations: Distillation and extraction

Text Book:

Unit Operations of Chemical Engineering,
Warren McCabe,

Julian Smith,

Peter Harriott
, 7
th

Edition
(McGraw Hill).

Reaction engineering

Types of reactions, rate of reaction, mechanism of reac
tions, Ideal reactors (batch, MFR, PFR), Design of
reactors for single and multiple reactions, Non
-
ideality in reactions, Non
-
isothermal and heterogeneous
reactions.

Text Book

Chemical Reaction Engineering Fundamentals Levenspiel
et al.
3
rd

edition, Wiley,
India.