Microbiology - Chabot College

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Chabot College

Fall 2011


Course Outline for Microbiology 1

MICROBIOLOGY


Catalog Description
:


1


Microbiology

5 units



Bacteria, fungi, protozoa, and viruses with an emphasis on their relationship to humans and disease.
Cultivation, control, metabolism
, body's defenses against disease, microbial genetics, laboratory tests, and
contemporary infectious diseases. Methods used in the laboratory include staining, investigation, cultivation,
identification of unknowns, and sensitivity testing. Prerequisite:
B
iology 31, and Chemistry 30A or Chemistry
1A (all completed with a grade of "C" or higher)
. Strongly recommended: Anatomy 1,
eligibility for English 1A. 3 hours lecture, 6 hours laboratory.

Typical contact hours: lecture 52.5, laboratory 105


Prerequisite

Skills
:


Before entering the course, the student should be able to:

Biology 31

1.

describe and apply the scientific method and how it is used by scientists to further scientific
knowledge;

2.

cite the characteristics and levels of organization ex
hibited by all living organisms;

3.

use the light microscope and dissecting scope;

4.

describe how cells/specialized cells are structured and function;

5.

describe basic cell metabolism;

6.

describe/contrast, mitosis and meiosis;

7.

describe structure, transmission and
expression of genes;

8.

explain the Darwinian concept of evolution as modified by modern scientific knowledge;

9.

describe how the modern (binomial) system names and classifies organisms.


Chemistry 30A or Chemistry 1A

1.

make unit conversions in the metric system;

2.

describe the structure of the atom;

3.

perform calculations using the mole concept;

4.

identify properties of states of matter;

5.

write balanced equations for chemical reactions including those in aqueous solution

and those involving elementary oxidation
-
reductio
n;

6.

describe gas laws qualitatively and quantitatively;

7.

define concentration units of solutions and use these definitions in problem solving;

8.

describe properties of solutions;

9.

interpret reactions according to acid
-
base theory;

10.

use the pH scale to compare ac
idity;

11.

write balanced net and total ionic equations;

12.

describe factors affecting the rates of reactions;

13.

describe types of nuclear radiation, isotopes and their half
-
life, nuclear reactions,

units, and medical/industrial uses;

14.

perform laboratory experiments

in an efficient, safe and purposeful manner;

15.

collect and analyze scientific data

16.

perform a titration
.


Chabot College

Course Outline for Microbiology 1, page
2

Fall 2011



Expected Outcome for Students:


Upon completion of the course the student should be able to:


1.

describe key aspects of the development of microbiology a
nd its significance to modern
microbiology;

2.

handle microbial cultures in a safe manner;

3.

demonstrate proficiency using the bright field microscope;

4.

list the main characteristics and structural components of viruses, prokaryotic, and eukaryotic
cells, and to

discuss their functions;

5.

purify and identify bacterial isolates based on metabolic profiles and microscopic

characteristics;

6.

explain the principles and interpretation of common bacteriological tests;

7.

describe the basic aspects of epidemiology;

8.

describe t
he conditions that lead to infectious disease development;

9.

investigate the antimicrobial susceptibility of bacterial isolates;

10.

explain the principles of antimicrobials’ mechanisms of action, and resistance development;

11.

explain the use of disinfectants, ant
iseptics, sanitizers and the mode of action of selected
examples;

12.

explain fundamental bacterial genetic principles and their relevance to disease development and
biotechnology;

13.

describe the main components and functions of the innate and acquired immunity;

14.

develop approaches to control the presentation of infectious diseases.


Course Content (Lecture):


1.

Introduction to microbiology


a.

Areas of study in microbiology


b.

Classification


c.

History

2.

Structure of bacteria


a.

Shapes and arrangements


b.


Flagella


c.

Fimbriae


d.

Capsules


e.

Cell wall


f.

Cell membrane


g.

Cytoplasmic inclusions

h.

Endospores

3.

Cultivation of bacteria


a.

Growth requirements


b.

Classification of media


c.

Obtaining and maintaining cultures

4.

Metabolism


a.

E
nzyme structure and function


b.

Physical factors that influence enzyme activity


c.

Structure of carbohydrates, proteins, lipids, nucleic acids


d.

Metabolism of glucose, proteins, lipids


e.

Aerobic and anaerobic respiration


f.

ATP synthesis and functio
n


g.

Structure of DNA and RNA


h.

Role of mRNA, tRNA, rRNA in protein synthesis


i.

Operon Model


Chabot College

Course Outline for Microbiology 1, page
3

Fall 2011



5.

Genetics


a.

Mutations, i.e., chromosome and gene


b.

Genotype, phenotype


c.

Conjugation



d.

Transformation


e.

Transduction


f.

Biotechnolo
gy and recombinant DNA technology

6.

Bacteria


a.

Classification and taxonomy


c.

Human diseases


d.

Methods of diagnosis

7.

Fungi


a.

Structure and reproduction


b.

Human diseases


c.

Growth requirements

8.

Protozoa


a.

Structure and reproduction


b.

Human diseases


c.

Methods of diagnosis

9.

Viruses


a.

Structure and nucleic acids


b.

Reproduction


c.

Human diseases

10.

Control of Microorganisms


a.

Sterilization, disinfection, antimicrobial agents


b.

Physical methods of microbial contr
ol


c.

Antibiotics and chemotherapeutic agents

11.

Immunology


a.

Nonspecific immunity, i.e., blood cells, lymphatic system, phagocytosis, tissue factors,
complement


b.

Specific immunity, i.e., antigens, antibodies


c.

Clonal selection Theory


d.

T and
B lymphocytes and their function

12.

Applications of immunology


a.

Agglutination tests


b.

Precipitin tests


c.

Monoclonal and polyclonal antibodies


d.

Neutralization tests


e.

Fluorescent antibody testing


f.

ELISA and Western
-
transfer techniq
ue


g.

Vaccines and passive immunity

13.

Epidemiology

a.

Rate of disease in a population

b.

Reservoirs and vectors

c.

Modes of transmission

d.

Pathogenesis

e.

Symbiotic relationships

f.

Nosocomial infections

14.

Contemporary Infectious Diseases


a.

AIDS


b.

Infectious hepatitis


c.

Viral respiratory infections

d.

Dental diseases

Chabot College

Course Outline for Microbiology 1, page
4

Fall 2011





Course Content (Laboratory Activities):


1.

Safety in the microbiology laboratory

2.

Use and care of microscope

3.

Preparation and staining of bacterial specimens for microscopic observ
ation

4.

Determination of bacterial morphology and cellular arrangements

5.

Aseptic and isolation techniques

6.

Staining procedures, i.e., simple, gram, acid
-
fast, spore, capsule, negative

7.

Morphological unknown

8.

Anaerobic culture methods

9.

Carbohydrate fermentation

10.

Re
spiration reactions

11.

Protein utilization

12.

Differential media

13.

Rapid identification methods (Enterotube II)

14.

Bacterial unknown

15.

Serial dilutions and counting bacteria

16.

Bacteriophages

17.

Effect of temperature on bacterial control

18.

Effect of ultra violet light on bacte
rial control

19.

Antibiotic testing
-
Kirby Bauer

20.

Electrophoresis

21.

Disinfectant testing

22.

Polymerase chain reaction (PCR) technique

23.

Epidemiology

24.

Throat culture

25.

Skin culture

26.

Pathogenic protozoa


Methods of
Presentation
:


1.

Lecture and discussion

2.

Observation, collec
tion and analysis of data

3.

Laboratory studies

4.

Articles from peer
-
reviewed medical/scientific journals

5.

Case studies


Assignments and Methods of Evaluating Student Progress
:


1.

Typical Assignments

a.

Lecture. Compare the cellular structure of gram po
sitive, gram negative, and acid
-
fast bacteria and explain how this relates to their capacity to cause disease in
humans.

b.

Laboratory exercises. Using microscopy and differential biochemical tests to identify
bacterial cultures.

c.

Laboratory exercise. Perform
ing the Kirby
-
Bauer test to determining the antibiotic
sensitivity of selected bacteria.


2.

Methods of evaluating student progress

a.

Lecture homework assignments (typically one per topic).

b.

Laboratory homework assignments (typically one per topic).

Chabot College

Course Outline for Microbiology 1, page
5

Fall 2011



c.

Minimum of

three midterm examinations.

d.

Minimum of three laboratory exams.

e.

Minimum two formal laboratory reports.

f.

Final examination.




Textbook(s) (Typical)
:


Microbiology, 10
th edition, Tortora, McMillian, 2010

Laboratory Experiments in Microbiology
, Johnson, Pears
on Benjamin Cummings, 2007


Special Student Materials
:


1.

Lab coat or apron

2.

Marking pen

3.

Colored pencils




CE C:My Documents/Outline/Outline_Micr1_fall_2010.doc

Revised 9/19/2010