Veer Bahadur Singh (CV)x - American Phytopathological Society

importantpsittacosisBiotechnology

Feb 20, 2013 (4 years and 3 months ago)

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1


VEER BAHADUR SINGH


Senior Research Fellow

Division of Biochemistry

Indian Agricultural Research Institute

New Delhi
-
110012





e
-
mail: vbsinghh@gmail.com




+91
-
995
8
339492




+91
-
9350563010



As research
scientist
, aspiring to explore and find new heights in the field of science through
novel experimental and analytical
approaches.


Academia

Doctor of Philosophy (Bioscience)



2012


Jamia Millia Islamia (A Central University), New Delhi
, India


Master of Science (Biotechnology)



2000
-
2002


62.3%

Specialization: Agricultural Biotechnology

Allahabad Agricultural
Institute
(Deemed University), Allahabad
, India

62.3%



Bachelor of Science

Honors (Agriculture)


1996
-
2000


62.87%

Specialization: Plant Science

C.S.J.M., University, Kanpur,
India




Research Experience

Doctorate Research:

Supervisor:

Dr. V.G. Malathi

Duration:

2009
-
2012

Work Place:

Advanced Centre for Plant Virology,
Division of Plant Pathology,


I
ndian
A
gricultural
R
esearch
I
nstitute
, New Delhi, India

Thesis title:


Studies on RNAi mediated resistance for the management of Mungbean yellow



mosaic India virus (MYMIV) in soybean


Nature of work:

Soybean is highly proteinac
e
ous among legumes and also good source of phosphorous, lecithin
and as
well as water soluble vitamins.
Soybean has importance in human dietary and
industrial
use. Yellow mosaic disease (YMD) of grain legumes

is a major constraint in the cultivation of
pulses and grain legumes in India.
Yellow mosaic disease (YMD), the most destructive disease of
soybean, caused by the viruses belonging to the Genus B
ogomovirus in the family
Geminiviridae
.

Yellow mosaic disease of soybean is caused by two viruses i.e.
Mungbean yellow mosaic virus

(MYMV) and
Mungbean yellow mosaic India virus

(MYMIV).
Viruses of family
Geminiviridae

have
typical geminate particle
morphology, encapsidating single stranded circular DNA of 2.5 to 3.0 kb


2


in size as genome. The viruses of the genus Bogomovirus infect dicotyledonous plants and are
transmitted by whitefly (
Bemisia tabaci

Genn.)
.

MYMIV have bipartite genome designated as
D
NA
-
A and DNA
-
B,
each of about 2.7 kb in size
.
Soybean cultivation was difficult in northern
India during 70s, because of YMD constraint.
To

exploit that potential, it is essential to overcome
productivity constraints of which YMD plays a major role. For control of YMD in soybean, the
pathogen should be identified, and managed by developing resistance variety. To achieve this
goal, following o
bjectives have been framed in this study.

1.

Survey, collection and maintenance of yellow mosaic
virus

isolates infecting Soybean.

2.

Molecular cloning of replication initiation protein gene and intergenic region of the virus
isolates.

3.

To study the variability a
mong different isolates and identify the conserved region in the
replication initiation protein gene and intergenic region.

4.

Development of RNAi constructs targeting replication initiation protein gene and intergenic
region.

5.

To develop transgenic soybean pl
ants with RNAi construct and their analysis.

The YMD infected
leaves

were collected
from major soybean growing region of India. The
replication initiation protein
gene
(Rep
, ORF AC1
) and intergenic region (IR) were PCR amplified
using specific primer. The
antisense construct
of Rep gene
and
hairpin construct
s

f
or Rep and IR
region
were made
. Three Indian cultivars were transformed by agrobacterium
-
mediated
transformation and regeneration protocol

standardized
.

The salient findings of the study are
summarized below:



Eight YMD infected samples were collected from

different geographical region of India.



Primers have been designed for antisense Rep, hairpin construct of Rep and IR region on the
basis of conserved re
g
ion.




Two
RNAi construct
targeting
Replicase gene (ORF AC1, antisense and hairpin) and one
targeting intergenic region (IR, hairpin)
of MYMIV
-
Sb was made in plant transformation vector

and confirming through sequencing. T
he efficiency of this RNAi construct was tested for
mitigation of dise
ase by co
-
agroinoculation along with the infectious clones of MYMIV
-
Sb.

In
the case of plants co
-
agroinoculated with RNAi construct, the symptom severity as well as the
percentage of infection was almost negligible
, confirming the inhibition of disease
dev
elopment by RNAi construct.



Transformation protocol was standardized for three Indian cultivars.

Fourteen T
1

lines were
confirmed for antisense Rep gene, thirteen T
0

lines for hairpin Rep and eighteen T0 lines for
hairpin IR gene incorporation.



Bioassay
experiment
were

performed
for 176 T
1

antisense Rep transgenic plants, 143 lines
were expressed resistant for the MYMIV.



RNAi construct made is effective

against the virus inoculation.
First in soybean, the proof of
concept of RNAi has been shown in this s
tudy.

Post Graduate Research:

Supervisor:

Dr. Radha Rama Devi

Duration:

2000
-
2002

Work Place:

Diagnostic Lab, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India



3


Thesis title:


Population based study of Spinal Muscular Atrophy using PCR based
RFLP



Nature of work:

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease,
characterized by deletion in the exons 7 & 8 of SMN gene (188 bp and 187 bp respectively) of
the SMN
t

gene mapped on the 5q11.2 to 13.3 chromosome
. A first time pilot study was
undertaken to asses SMA incidence in neonatal population of Indian using PCR based RFLP
analysis as a tool.

The salient findings of the study are summarized below:



DNA of blood samples from 67 neonates and 15 referred cases
were analyzed for detection
exons 7 & 8 of SMN
t
gene. PCR of exons 7 & 8 of SMN gene and subsequent RFLP
standardized.



Of the 15 referred cases 3 were detected with deletion of 188 bp on exon 7 and 187 bp on
exon 8 of SMN gene. However no positive cases w
ere detected in neonatal DNA studies.



The results of the study indicated that DNA analysis of larger neonatal population is required
for early detection, genetic counseling, prevention and therapy.

Current Research Profile
:

Supervisor:
Dr. Archana
Sachdev

Currently working as Senior Research Fellow

(15
th

February, 2012
-
Till date)

Project:


Use of RNAi technology in developing low phytate soybean and rice

.

Work place:

Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, India

Nature of work:

Cereal
grains and oilseeds contain large quantities of phytic acid, which provides
myo
-
inositol
and phosphorus required during seed germination and seedling

establishment. Phytic acid has
a negative impact on animal nutrition and the environment. The otherwise nutritionally
adequate phosphorus content of cereal grains and oilseeds is bound in phytic acid and
therefore not available to monogastric animals that

lack sufficient phytase in their digestive
tract for optimal phosphorous nutrition.
ATP bin
d
ing cassette
(ABC) transporter is a key
contributor to phytic acid accumulation in soybean seeds. Phyitc acid level can be reduced by
silencing the ABC gene

which
engineer the dominant, seed specific block in phytic acid
accumulation.


Key Performance area
:



RNAi construct
were designed
using
ABC transporter gene

to reduce the level of phytic acid in
developing seeds

of soybean
.



Agrobacterium

mediated transformation using the RNAi construct and analysis of putative
tran
s
formant
s

for gene incorporation.



Analysis the level of phytic acid by MegaZyme kit, modified
colorimetric

method and HPLC.




4


Previous

Research Profile
:

Supervisor
:

Dr. V.G.
Malathi

(I)


Senior Research Fellow
(
2
3
rd

Sept., 20
10
-
14 Feb., 2012)

Project:


Net Work Project o
n Transgenics in Crops (NPTC)”


Work Place:

Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural
Research Institute (IARI), Pusa

Campus, New Delhi, India.

(II)


Senior Research Fellow
(3
rd

Sept., 2007
-
8
th

August
, 20
10
)

Project:


Molecular analysis of virulence of tomato leaf curl viruses: role of satellite DNA β”

Work Place:

Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural
Research Institute (IARI), Pusa Campus, New Delhi, India.

Key Performance area
:



Molecular characterization of monopartite (DNA A and DNA β) and bipartite (DNA A and DNA
B)

tomato leaf curl

begomoviruses through Rolling Circle Amplification (RCA).



Infectivity analysis through Agroinoculation of Partial Tandem Repeat constructs (PTR) of the
clones and through biolistic delivery of rolling circle amplified products, of tomato
begomoviruses.



Screening of susceptible and resistance tomato genotypes against tomato begomoviruses
through whitefly (
Bemisia tabaci
) transmission.



Construction of cDNA library of susceptible and resistant tomato variety
,

binding activity of
cDNA

with satellite conserved region

(Yeast one
-
hybrid system)

and βC1 of betasatellite

(Yeast two
-
hybrid system)

was analyzed
.

Supervisor:

Dr. D.V. Singh and Dr. Rashmi Aggarwal

(I)

Research Associate

(16
th

Nov., 2006
-

3
rd

Sept. 2007)

and
Junior Research Fellow

(4
th

April 2005
-

14
th

Nov. 2006)

Project:


Toxin and DNA based variability among the isolates of
Bipolaris sorokiniana
causing
spot blotch of wheat and induction of defense genes involved in host pathogen interaction”

Work Place:

Fungal molecular and Plant pathology laboratory,
Division of Plant Pathology,
Indian Agricultural Research Institute (IARI), Pusa Campus, New Delhi, India.

Key Performance area
:



Bipolaris sorokiniana

(teleomorph
Cochliobolus sativus
) is the causal agent o
f common root
rot, leaf spot disease, seedling blight, head blight, and black point of wheat and barley.



Total 113 isolates
of
spot blotch

were collected from 6 zone and characterized by different
molecular markers. Many molecular markers is applied in th
e present study like RAPD, URP,
ISSR, ITS

and

RELP. After studying the result, the each zone are preparing separate cluster.



On the basis of these data SCAR marker is developed for the estimation of
Bipolaris
spp. The
SCAR marker has industrial importance

and this marker is to be commercialize for the quick
detection of fungal disease.

(II)


Senior Research Fellow

(28
th

Nov. 2003
-

3
rd

April 2005)

Project:
“Development of bioformulation for control of leaf blight in wheat under rice wheat
cropping system”



5


Work place: Fungal molecular and Plant pathology laboratory,
Division of Plant Pathology, Indian
Agricultural Research

Institute (IARI), Pusa Campus, New Delhi, India.

Key Performance area
:



Trichoderma

species have been investigated as biological control agents against
Bipolaris
sorokiniana

and
Fusarium graminiarum
. There morphological differences among different
species and isolates of the same species.



We used
27
Randomly Amplified Polymorphic DNA (RA
PD)
-
PCR and ITS primers to estimate
genetic variation among isolates of
Trichoderma reesei
(2 isolates)
,
Trichoderma viride
(10
isolates)
,
Trichoderma pseudokoningii
(1 isolate)

and
Trichoderma harzianum

(1 isolate)
.



Polymorphisms were found between speci
es. RAPD analysis suggested that, two strains of
Trichoderma viride
(T V 5
-
2 & 2211) and two other strains of
Trichoderma viride
(TCT
-
10 &
T2) are 88% same. Amplification of the ITS region produced a single band of approximately
600 bp for all the isolates
. Although there was differences in branching between dendogram
derived from RAPD supported the distinction of 4
Trichoderma

species, with the
T. viride
isolates
grouping together and showing differences at an intraspecific level
.



Working Advantages



Expertise in cloning and understanding the genome organization of begomoviruses causing
diseases of national importance

Mungbean yellow mosaic India virus

(MYMIV), Tomato leaf
curl viruses (ToLCVs)



Efficient in making infectious constructs and in various
screening techniques (agroinoculation,
agroinfiltration, biolistic delivery, sap inoculation and whitefly transmission) to identify the
resistance source to leaf curl and yellow mosaic begomoviruses



Molecular characterization of
Bipolaris sorokiniana

using

universal rice primer (URP) and
Trichoderma

spp. Using RAPD primers. Development of SCAR marker for
Bipolaris
sorokiniana
.

Pathogenesity test of
Bipolaris sorokiniana

on wheat cultivars.



Disease Diagnosis and identification, Disease assessment and screeni
ng.



Culturing and handling of fungal pathogen/biocontrol agent.



Well conversed with NASH, Southern, Northern (siRNA detection) Western blotting and ELISA
techniques



Expertise in p
lant tissue culture techniques of genetic transformation using
A. tumefacie
ns

for
micropropagation, callus initiation, maintenance and regeneration



Contemporary Methodologies:
Rolling circle amplification (RCA), Real
-
time quantitative
PCR analysis, PCR mutagenesis, post transcriptional gene silencing and silencing suppression
assays, cDNA library construction, Yeast one
-
hybrid system, Yeast two
-
hybrid system



Bioinformatic skills
:
Primers designing by manual and using Primer 3.0 software; Analysis
of sequences using GENERUNNER, BioEdit, MEGA 5.0; Recombination analysis (RDP3)


Professional Experience



Resource person for the Training

on “Application of Biochemical and Molecular tec
hniques
for characterization of Plant pathogens” organised by Centre for Advanced Studies, Division of
Plant pathology, Indian Agricultural Research Institute, New Delhi.



6




Resource person for the Training

on “Biological control of Plant Pathogens” organised

by
Centre for Advanced Studies, Division of Plant pathology, Indian Agricultural Research
Institute, New Delhi.



Resource person for the
training on “Biocontrol Strategies for Management of Plant
Pathogens” organised by Centre for Advanced Studies, Divisio
n of Plant pathology, Indian
Agricultural Research Institute, New Delhi.

Training/Workshop attended



One month training on
Tissue culture
, at Co
-
operative Rural Development Trust (CORDET),
Motilal Nehru Farmer’s Training Institute, Ghiyanagar, Phulpur,
Allahabad from March 2000 to
April 2000.



Two months training on
monitoring the pollution level during Mahakumbh Mela in
Sangam at Allahabad
, at Department of Chemistry, Allahabad Agricultural Deemed
University, Allahabad from January 2001 to February 2001.



Three months training on
Study of Cyanobacterial strains
, at Department of Botany,
University of Allahabad, Allahabad from June 2001 to August, 2001.



One day orientation program
on Radioisotopes Use and Safety,
at the Nuclear Research
Laboratory, Indian A
gricultural Research Institute, New Delhi on 7
th

August, 2006.

Publications

Full research papers:

International publications:

1.

Rashmi Aggarwal,
V. B. Singh
, Renu Shukla, Malkhan Singh Gurjar, Sangeeta Gupta and
Tilak

R. Sharma (2010). URP
-
based DNA Fingerprinting of
Bipolaris sorokiniana

Isolates
Causing Spot Blotch of Wheat. J Phytopathol. 158 (4): 210
-
216.

2.

Neha Tiwari, Padmalatha K.V.,
Singh V.B.
, Haq Q.M.I., and Malathi

V.G. (2010). Tomato leaf
curl Bangalore virus (ToLCBV): Infectivity and enhanced pathogenicity with diverse
betasatellite. Arch. Virol.
155 (
8):
1343
-
1347.

3.

R. Aggarwal, S. Gupta, S. Banerjee and
V.B. Singh

(2011). Development of a SCAR marker
for detection of
Bipolaris sorokiniana

causing spot blotch of wheat.
Canadian Journal of
Microbiology
, 2011, 57:(11) 934
-
942.

4.

Neha Tiwari,
Veer B. Singh
, P.K. Sharma and V.G. Malathi (2012
)
.

Tomato leaf curl
Joydebpur

virus


a monopartite Begomovirus causing severe leaf curl in tomato in West
Bengal

(Communicated).

5.

Veer B. Singh
, Q.M.I.Haq and V.G. Malathi (2012).
A RNAi approach targeting Rep gene of
Mungbean yellow mosaic India virus to develop resistance in soybean

(Communicated)
.



National publication:

6.

Rashmi Aggarwal,
V. B. Singh
, M. S. Gurjar, Sangeeta Gupta and P. Srinivas (2009).
Intraspecific variations in India isolates of
Bipolaris sorokiniana

infecting wheat based on
morphological, pathogenic and molecular characters. Indian Phytopathology
,

62 (4): 449
-
460.

7.

Rashmi Aggarwal, Sangeeta Gupta,

V. B. Singh

and Sapna Sharma (2011).
Microbial
detoxification of pathotoxin produced by spot blotch patho
gen
Bipolaris sorokiniana

infecting wheat. J. Plant Biochem. Biotechnol, 20 (1): 66
-
73.




7


Chapters in training manual:

8.

V. B. Singh
, Aradhika Tripathi, Kirti Bhalla and Renu. 2006. Isolation of fungal genomic DNA.
In: Training course on Advanced Techniques
in Plant Disease Diagnosis and Management
-

A practical manual. Center for Advanced Studies, Division of Plant pathology, Indian
Agricultural Research Institute, New Delhi pp
-

10
-
12.

9.

V. B. Singh
, Vandana Sharma, Sangeeta Gupta and Kirti Bhalla. 2006. Quanti
fication of DNA.
In: Training course on Advanced Techniques in Plant Disease Diagnosis and Management
-

A practical manual. Center for Advanced Studies, Division of Plant pathology, Indian
Agricultural Research Institute, New Delhi pp
-

13
-
15.

10.

V.G. Malathi,
Veer Bahadur Singh
and P. Jyothsna, 2010. Viral genomics and Transgenic
development
-

A laboratory manual.
Center of Advanced Faculty Training (CAFT),
Advanced centre for plant virology, Division of Plant pathology, Indian Agricultural
Research Institute, N
ew Delhi pp
-

10
-
12.

Book chapter:

11.

Aggarwal Rashmi, Sangeeta Gupta and
V.B. Singh
, 2008. Secondary metabolites produced
by biocontrol agents and their role in plant disease management.
In
: Ecofriendly
Management of Plant Disease. (Eds. Shahid and Udit
Narain).
Daya Publishing House New
Delhi
-
35
, pp 397
-
415.

Invited lectures/orals/posters:

National:

1
2
.
Siddiqui, Shahid Ali, Chattree, Amit and
Singh, Veer Bahadur
(2001). Impact of mass
Bathing on heavy metals, concentration, pH, and electrical conducti
vity of sacred river
during 'Mahakumbh 2001 at Allahabad. Oral presentation in National symposium on
Plant Diversity and Biotechnology. Department of botany, Patna University, Patna,
October 9
-
10, 2001: 10.

1
3
. Ahammed, S. Khayum, Aggarwal Rashmi, Singh,

D. V. and
Singh V. B.
(2004). Optimising
nutritional conditions for the mass multiplication of
Chaetomium globosum
, an efficient
biocontrol agent of
Bipolaris sorokiniana
. Poster presentation in National symposium on
Crop Surveillance: Disease Forecasting

and Management. Division of Plant Pathology,
IARI, New Delhi February 19


21, 2004 : 103.

1
4
. Aggarwal Rashmi,
Singh, V. B.,
Gupta,

Vishnu Kumar and
Singh, D. V. (2005). Pathogenic
and Molecular variability in
Bipolaris sorokiniana
. Oral presntation

in National symposium
on sustainable Pant Protection Strategies

: Health and Environmental Concerns. Division
of Plant Pathology, Dr. Balashaheb Savant Konkar Krishi Vidyapeeth, Dapoli, Ratnagiri
(Maharastra) October 15
-
17, 2005

: 67.

15.

Aggarwal Rashmi,
Si
ngh, V. B.,

Jahani, Mehdi, Tripathi, Aradhika (2005).

Variability in
Bipolaris sorokiniana
, causing spot blotch in wheat. Submitted for the oral session in
National Symposium on Fungal Biodiversity, Biotechnology and Bioinformatics
(NSFBBB). Sri Bhagawan M
ahaveer Jain College, Centre for Post Graduate Studies,
Bangalore February 2 & 3, 2006: 32.

16.

Aggarwal, R.,
V. B. Singh,

Rajbeer Singh and Aradhika Tripathi (2006). Molecular
characterization of
Trichoderma
spp. by PCR
-
RAPD and ITS region amplification. Oral

presented in 58
th

Annual meeting Indian Phytopathological Society held at Siliguri, 31
st

Jan.
-
3
rd

Feb., 2006.

1
7
. Aggarwal Rashmi,
Singh V. B.

and
Aradhika Tripathi. (2007). “Analysis of genetic variability
in
Bipolaris sorokiniana

using Universal Rice Primer (URP)”. Oral presentation in 9
th



8


Indian Agricultural Scientists and farmers congress, BIOVED, Allahabad. January 29


30, 2007: 77.

1
8
.
Aggarwal Rashmi, Aradhika Tripathi and
Singh V. B.
(2007)
. “Molecular characterization of
Tilletia indica usingh URP
-
PCR technique”. Poster presentation in National symposium on
Plant Diseases and its Management, Rani Durgavati University, Jabalpur (M.P.), January
16


18, 2007: 103.


International:

1
9
.
V.B.Singh
,
Neha Tiwari
, Haq.Q.M.I.,
Jyothsna P., Richa S., Archana K and V.G.Malathi
(2009). Infectivity of a new begomovirus:
Tomato leaf curl Aurangabad virus
(ToLCAuV). The 6th Solanaceae Genome Workshop, Nov 8
-
13,2009, Le Meridien, New
Delhi, India.

20
.
Neha Tiwari,
V.B.Singh
, Haq.Q.M.I
., Jyothsna P., Richa S., Archana K and V.G.Malathi
(2009). Infectivity of
Tomato leaf curl Bangalore virus
. The 6th Solanaceae Genome
Workshop, Nov 8
-
13,2009, Le Meridien, New Delhi, India.

21
. Haq.Q.M.I
.
,
Neha Tiwari
., Jyothsna P.,
V.B.Singh
, Richa S., A
rchana K and V.G.Malathi
(2009). Cloning and Infectivity of tomato begomoviruses through Rolling Circle
Amplication (RCA) of the genome. The 6
th

Solanaceae Genome Workshop, Nov 8
-
13, Le
Meridien, New Delhi, India.

2
2
. Richa Shukla, Haq.Q.M.I.,
Neha Tiwari.
,
V. B.Singh
, Jyothsna P., Archana K and V.G.Malathi
(2009). RNA silencing suppressors encoded by beta satellite of tomato begomoviruses.
The 6th Solanaceae Genome Workshop, Nov 8
-
13,2009, Le Meridien, New Delhi, India.

2
3
. Jyothsna P, Archana K., Haq.Q.M.
I.,
Neha Tiwari
, Richa Shukla,
V.B.Singh

and V.G. Malathi
(2009). Realtime PCR for the quantification of Tomato leaf curl virus in tomato plants
and its vector
Bemisia
tabaci. The 6th Solanaceae Genome Workshop, Nov 8
-
13,2009,
Le Meridien, New Delhi,
India.

2
4
. Sulaiman K Fadil, Jyothsna P, Haq.Q.M.I
.
,
Neha Tiwari
, Richa Shukla,
V. B. Singh
, Archana.
K. and V.G.Malathi (2010). Universal primers based on conserved region of coat protein
for the detection of whitefly transmitted begomoviruses. Internatio
nal Conference on
“13th Congress of the Mediterranean Phytopathological Union”. June. 20
-
25, CRA
-
PAV
Plant Pathology Research Centre, Via C. G. Bertero, 22, 00156 Rome, Italy.

2
5
.
Neha Tiwari
,
V.B. Singh
, P.K. Sharma and V.G. Malathi (2010).
Tomato leaf cu
rl Joydebpur
virus


a monopartite Begomovirus causing severe leaf curl in tomato in West Bengal.
Conference on Whitefly and Thrips Transmitted Viruses, Aug 27
-
28, University of Delhi
South Campus, New Delhi, India

2
6
. Malathi V.G., Jyothsna P, QMI Haq,
Ne
ha Tiwari,
Richa Shukla,
V.B. Singh
, Archana Kumari.
(2010). Pathogenicity of mono and bipartite Tomato leaf curl viruses in India.
Conference on Whitefly and Thrips Transmitted Viruses, Aug 27
-
28, University of Delhi
South Campus, New Delhi, India

Nuleotid
e sequences published:

2
7
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. 5137 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ242474.



9


2
8
. Aggarwal,R.,
Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. bs05 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Access
ion No. DQ286764

2
9
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. bs25 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal

RNA gene, partial
sequence. Accession No. DQ286763.

30
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. bs55 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2,
complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ242476.

31
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. bs18 18S ribosomal RNA gene,
partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene,

and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence. Accession No. DQ242475.

3
2
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. 5137 18S ribosomal RNA gene,
partial sequence; internal transcribe
d spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence.

3
3
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. 4922 18S ribosomal RNA gene,
partial sequence; interna
l transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence.

3
4
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris

sp. 4805 18S ribosomal RNA gene,
partial seque
nce; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial
sequence.

3
5
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris sorokiniana

strain 9 18S ribosomal

RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene,
and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene,
partial sequence. Accession No. DQ229952.

3
6
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris sorokiniana

strain 64 18S
ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal
RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal
RNA gene, partial sequence. Accession No. DQ22
9951.

3
7
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris sorokiniana

strain 27 18S
ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal
RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosom
al
RNA gene, partial sequence.
Accession No.

DQ229950.

3
8
. Aggarwal,R., Jahani,M. and
Singh,V.B.
(2005).
Bipolaris sorokiniana

strain 27 18S
ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal
RNA gene, and internal transcr
ibed spacer 2, complete sequence; and 28S ribosomal
RNA gene, partial sequence.
Accession No.

DQ229950.

3
9
. Aggarwal,R.,
Singh,V.B.,

Jahani,M. and Singh,D.V. (2005).
Trichoderma viride

strain 1433
internal transcribed spacer 1,
partial sequence; 5.8S ribos
omal RNA gene, complete
sequence; and internal transcribed spacer 2, partial sequence, partial sequence.
Accession No.
DQ217841
.

40
. Neha, T.,
Singh, V.B.

and Malathi, V.G. (2010).
Tomato leaf curl Bangalore virus isolate 18
segment DNA
-
A, complete sequenc
e.
Accession No. GU474418.




10


Personal details

Date of birth






:
10
th

March, 1979

Father’s Name





:
Mr. J.S. Singh

Nationality






: Indian

Marital status






:
Married

Languages known




:
English

&

Hindi


References:

Dr. V.G. Malathi

Emeritus Scientist (CSIR),

Plant Virology Unit,

Division of Plant Pathology,

Indian Agricultural Research Institute,

Pusa Campus,

New Delhi


110012, India

vgmalathi@rediffmail.com


Phone

No.
+91
-
9717843963


Dr. Rashmi Aggarwal

National Fellow

Division of Plant Pathology,

Indian Agricultural Research Institute,

Pusa Campus,

New Delhi


110012, India

rashmiiari@yahoo.com


Dr.
D.V. Singh

Ex
-
Head
,

Division of Plant Pathology,

Indian Agricultural Research
Institute,

Pusa Campus,

New Delhi


110012, India






Date:

Place:









(Veer Bahadur Singh)