BIOTECHNOLOGY LEARNING OBJECTIVES Being able to answer the following is essential for success on this test!

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Oct 23, 2013 (3 years and 7 months ago)

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BIOTECHNOLOGY LEARNING OBJECTIVES

Being able to answer the following is essential for success on this test!

1.

What are restriction enzymes?

Where did they come from?

2.

What is a restriction site?

3.

A restriction enzyme cuts between cytosine and guanine in this
DNA sequence. How many nucleotides will its
sticky end have? Draw in a line showing the cut.

A
CG
ATCGT

TGCTA
GC
A

4.

What is gel electrophoresis? How does it work?

5.

If a molecule travels toward the positive electrode on a gel, is it positively or negatively
charged?

6.

Describe how molecules move on a gel based on size.

7.

On the back of this paper, use a gel diagram to explain how a person a person may or may not have committed a
crime. You can make up the scenario and the diagram yourself!

8.

What is PCR? What is

it used for?

9.

What are DNA fingerprints?

10.

What molecular and physical tools are needed to make a DNA fingerprint?

11.

How can DNA fingerprints be used to identify possible crime suspects?

12.

What is a clone? Contrast clone and transgenic organism.

13.

Describe geneti
c engineering. How is it related to recombinant DNA?

14.

Draw a plasmid. What type of organism contains plasmids?

15.

Describe how a gene of study can be inserted into a plasmid.

16.

What is transformation and why is it used in science?

17.

Define antibiotic resistance.

How is it used during transformation procedures?

18.

Why are bacteria used in transformation procedures?

19.

Why is genetic screening useful?

20.

What limits the usefulness of gene therapy, in other words, why can gene therapy be used to treat all ailments?




BIOTE
CHNOLOGY LEARNING OBJECTIVES

Being able to answer the following is essential for success on this test!

1.

What are restriction enzymes? Where did they come from?

2.

What is a restriction site?

3.

A restriction enzyme cuts between cytosine and guanine in this DNA s
equence. How many nucleotides will its
sticky end have? Draw in a line showing the cut.

A
CG
ATCGT

TGCTA
GC
A

4.

What is gel electrophoresis? How does it work?

5.

If a molecule travels toward the positive electrode on a gel, is it positively or negatively charg
ed?

6.

Describe how molecules move on a gel based on size.

7.

On the back of this paper, use a gel diagram to explain how a person a person may or may not have committed a
crime. You can make up the scenario and the diagram yourself!

8.

What is PCR? What is it u
sed for?

9.

What are DNA fingerprints?

10.

What molecular and physical tools are needed to make a DNA fingerprint?

11.

How can DNA fingerprints be used to identify possible crime suspects?

12.

What is a clone? Contrast clone and transgenic organism.

13.

Describe genetic eng
ineering. How is it related to recombinant DNA?

14.

Draw a plasmid. What type of organism contains plasmids?

15.

Describe how a gene of study can be inserted into a plasmid.

16.

What is transformation and why is it used in science?

17.

Define antibiotic resistance. How

is it used during transformation procedures?

18.

Why are bacteria used in transformation procedures?

19.

Why is genetic screening useful?

20.

What limits the usefulness of gene therapy, in other words, why can gene therapy be used to treat all ailments?