Tim Tripp Director, Biotechnology Resource Center 4/17/2013

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Oct 22, 2013 (3 years and 9 months ago)

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Tim Tripp

Director, Biotechnology
Resource Center

4/17/2013



The Biotechnology Institute


General introduction


Faculty



The Biotechnology Resource Center


Services


Equipment


Training

Outline

Provides advanced research, training, support,

and university
-
industry interaction

in biotechnology.

Established in 1983, the
BioTechnology Institute is a joint
venture of the College of Biological
Sciences and the College of Science
and Engineering.


29 years of
Progress in
Biotechnology

The BioTechnology Institute Continues to
be a Cornerstone of University
-
Industry
Interaction in the LifeSciences

Renewed support from the
University of Minnesota

Continuous improvement
and transformation


The College of Biological
Sciences (CBS)


The College of Science and
Engineering (CSE)


The Academic Health Center
(AHC)


The College of Food,
Agricultural, and Natural
Resource Sciences (CFANS)

The BioTechnology Institute
faculty represent four colleges
at the University of Minnesota:

COLLEGES



Biochemistry Molecular Biology and
Biophysics



Bioproducts & Biosystems Eng.



Center for Drug Design



Chemistry



Chemical Engineering and


Materials Science



Civil Engineering



Ecology, Evolution, & Behavior



Genetics, Cell Biology, and Development



Mechanical Engineering



Microbiology



Plant Biology



Soil, Water & Climate

The BioTechnology Institute draws
faculty from the departments of:

DEPARTMENTS

BioTechnology Institute faculty
have broad expertise in the areas
of:


Biocatalysis



Metabolic engineering/


microbial physiology



Combinatorial biology



Synthetic ecology



Population dynamics


Molecular Biology



Proteomics, and



Focused expertise in defined
areas


such as bioremediation,


biomaterials, biosensors, and


bioinformatics.



Yiannis Kaznessis

Ping Wang

Michael

Sadowsky

Director

Daniel Bond

Robert Brooker

Antony Dean

Mark Distefano

Jeffrey Gralnick

Wei
-
Shou Hu

Romas Kazlauskas

Arkady Khodursky

C. Schmidt
-
Dannert

Burckhard Seelig

Friedrich Srienc

Michael Travisano

Lawrence Wackett

FACULTY

Gary Dunny

The BioTechnology Institute
faculty have broad expertise in the
areas of:



Bringing together this expertise to
expand knowledge in new and
developing fields of commercially
relevant life sciences.


Finding new applications for
existing technology


Alptekin Aksan

Brett Barney

Raymond Hozalski

George Karypis

Timothy LaPara

Igor Libourel

Paige Novak

Christine Salomon

Fransico Diez
-
Gonzalez

Kechun Zhang

Bo Hu

Gary Muehlbauer

BTI faculty conduct research

over a broad spectrum of

disciplines, including:




Biocatalysis



Metabolic pathway






engineering



Genetics and cell biology



Functional genomics



Biodegradation of hazardous materials



Molecular evolution



Biological diversity



Green chemistry



Protein engineering



Development of biofuels and biopolymers
from renewable resources.

RESEARCH

Important aspects of the
BioTechnology Institute
include:




The Biocatalysis Initiative
-

Synthetic Ecology




Graduate Program in

Microbial Engineering




Scientist Exchange Program




Biotechnology NIH Training
Grant

INITIATIVES AND PROGRAMS

CORE VALUES

The BioTechnology Institute
is a multidisciplinary and
multi
-
departmental institute
guided by three core values:



Conduct innovative,
relevant research




Provide professional
training




Serve as a resource for
the University and industry


The BioTechnology Institute has
invested significantly in
facilities with updated
equipment.


Facilities and services available to
students and researchers
include the:



The Biotechnology Resource
Center


Pilot plant


Development lab




High Throughput Biological
Analysis Facility

FACILITIES & SERVICES

Serves as a Resource

The BRC

Biotechnology Resource Center

A service provided by the Biotechnology Institute

Fermentation/Cell Growth


Cultivation of bacteria, yeast &
algae


Process development &
optimization


Pilot
-
scale production

Recombinant Protein Expression


E. coli

& other bacterial expression
systems


Pichia pastoris

&
S. cerevisiae

Downstream Processing


Recovery and purification of
proteins & small molecules

GMP Capabilities


Fully established quality system


Production of Phase I and Phase II
clinical trial material

Facility

The
5000
-
square
-
foot
facility is
located on the University of
Minnesota’s St. Paul campus.

Equipment includes:


14 fermenters with volumes of

6L, 10L, 20L, 75L, 300L, and
550L


5 x 20L light
-
ring
photobioreactors


batch and continuous
-
flow
centrifuges


microfluidizer, high
-
pressure
homogenizer, & French press


bench
-

to pilot
-
scale ultra
-
filtration units


bench
-

to pilot
-
scale
chromatography


freeze dryer

The Biotechnology Resource
Center

Services:


Molecular biology

(cloning, site
-
directed
mutagenesis, expression vector construction,
sequence verification)


Recombinant protein expression


(e.g.
E. coli, Pichia pastoris
)


Fermentation process development

&
scale
-
up


(bacteria, yeast & fungi)


Animal Cell Culture


Downstream processing

(centrifugation, cell
breakage,

ultra
-

& micro
-
filtration, chromatography, freeze
drying)


Customers


Ajinomoto


Aldeveron


Alvine
Pharmaceuticals


Monsanto


Training Through the BRC


MicE 5355


Week long lab course


MT 3141


Half the semester in the lab


Custom programs


Multiple proposals

The Next Generation of
Biotechnologists


Work Study Program for undergrads



Worthington Middle School 8
th

grade
Science Club

Partners


Biomedical Genomics Center


Center for Mass Spectrometry


Proteomics Analysis Core


Imaging Center

Biomedical Genomics Center

Services & Instrumentation :


DNA sequencing ABI PRISM™ 3730xl DNA Analyzer ,
Illumina GAIIx and HiSeq 2000, Roche GS FLX (454
Sequencing)


Single
-
nucleotide polymorphism (SNP) analysis &
mutation detection


Real Time PCR (ABI 7700)


Micro/Macro array spotting and scanning (Biorobotics
Total Array System, Packard Scanner, Affymetrix
system)


RNA analysis (Agilent Bioanalyzer 2100)



Plasmid preparations (Qiagen 8000 robot, Hydra liquid
handlers)


Center for Mass
Spectrometry in the Life
Sciences

Services
:


Identification, and quantification of proteins and small
molecules


Protein & peptide sequencing


Identification of posttranslational modification of proteins

Instrumentation:


2x Thermo LC
-
Q w/ APCI & ESI


1x Thermo LT
-
Q, linear ion trap, ESI


3x ABI Qstar (2x ESI, 1x MALDI), 2D Nanoflow HPLC


ABI Qtrap, linear ion trap, capillary LC


LC Packings Probot MALDI Spotter w/ Michrome LC


Bruker MALDI
-
TOF up to 350 kD



Kratos high resolution GC
-
MS (w/ direct inlet tube)


Varian GC
-
MS (ion trap w/ EI and CI)

Proteomics Analysis

Core

Services & Instrumentation:


2D gel electrophoresis


DIGE with Typhoon Scanner (red & green laser)


High
-
throughput spot picking (Genomics Solution, ProPic 2)


Automated protein digestion (Genomics Solution, ProPrep 2)


2D HPLC analysis (ProteoSep)


Isotope Coded Affinity Tagging (ICAT)


All units work in conjunction with mass spec analysis in the
Center for Mass Spectrometry in the Life Sciences

Summary


BTI
-

Leading life science researchers


BRC
-

Leading Biotechnology services


Resources


Teaching


Industry interaction



Please come visit