Introduction to Cell Biology and Genetics Genetic Engineering and ...

giantsneckignoredBiotechnology

Dec 12, 2012 (4 years and 8 months ago)

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Bio102 :
Introduction to Cell Biology and Genetics

Genetic Engineering

and Gene Therapy


Key Terms
:


Gene Therapy

Retroviral vectors

Genetically Modified Food

Vector

Somatic cell gene therapy

Roundup
-
Ready Crops

Liposome

Germline gene therapy

Bt

crops

Adenoviral vectors




Key Figures:

Figure 20.22

Lecture Outline:

Human Gene Therapy


correcting human genetic diseases

Insertion
, m
odif
ication or removal
of a gene


Vectors


Direct injection of the target cell


Liposome


Viral vectors



DNA viruses
(such as adenovirus)

Retroviruses
(similar to HIV)


First gene therapy experiment in humans began in 1990

Remains expeimental


Problems


Insertional mutagenesis


Immune response


Multigene disorders


Correct tissue targeting


Efficiency


Ethical Issues


wh
at is normal? disease vs. disability

vs. difference
?


somatic vs. germline gene therapy


expense


Genetically Modified Food


Ti
-
plasmid to modify plant cells


any single plant cell can be used to reproduce an entire plant (cloning)


“Roundup
-
Ready” crops


Bt

crops


extremely common