Biocore 304: Cellular Biology Laboratory
-
Spring
201
1
Schedule
Your grade will be based on the assignments listed below. Note that the Subcellular Fractionation paper and Signal Transduct
ion
Poster are group grades. Check (√) assignments are scored simply adequate or inadequate; papers are graded using the criteria
d
escribed in the
Biocore Writing Manual
. Papers & assignments must be handed in on time unless you have contacted your TA
ahead
of time
to request an extension due to emergency or extenuating circumstances. Otherwise, we will deduct one grade per weekday
it is
late from the grade you would have received (
e.g.
, A
-
>AB for one day late). Note that even an F paper (one week late) counts more
than 0 (not handed in at all) when we total the final grades at the end of the semester. Late reports should be given
directly to your
TA, Janet Batzli or Michelle Harris (not put in a mailbox or submitted electronically).
Lecture topics
Week/
(date)
Lab Topic
Lab Activities/Assignments
Weight
Imaging, cell
composition
1 (Jan.
1
8
-
2
1
)
Microscopy:
Image Formation
Realign messed up microscope
–
do in lab
Cell observations/diagrams
–
do in lab
Macromolecule
structure/function,
organelles,
bioenergetics
2 (Jan.
2
4
-
2
8
)
Subcellular
Fractionation
Subcellular Fractionation pre
-
lab
due in disc.
Microscopy assignment
(produce hand drawings
with figure legends) due in lab
2
%
3%
Enzyme catalysis,
membranes &
transport, metabolic
pathways & role of
ATP
3 (
Jan
31
-
Feb
4
)
Enzyme I:
Alkaline
Phosphatase &
MM Kinetics
Enzyme pre
-
lab assignment due in disc.
Teams decide final
[
S
]
in disc
Subcell. Fract. Paper
(1/pair)
due in lab
Graphs & calculations for K
m
, V
max
–
do in lab
2
%
5
%
Glycolysis, anaerobic
energy metabolism, fat
metabolism, citric acid
cycle
4 (Feb.
7
-
1
1
)
Enzyme II:
Extended
Literature
Search/
Bioinformatics/
Statistics
Complete BLAST
&
ClustalW eTeach
es
and
Jmol
tutorial
& turn in answers to questions
at
beginning
of disc
Extended literatu
re search
(in disc)
Data Analysis pre
-
lab Part I assignment
due
beginning of lab
PL
AP physical
models
; Consurf analysis
(in lab)
Molecular structure/function:
E. coli
AP mutation
activity
(in lab)
Introduce t
-
tests, online stats materials (in lab)
Generate
enzyme
question, brief rationale,
hypothesis, tentative expt. design (1/team)
Teams sign up
for formal presentation feedback
2%
Electron transport &
ATP generation,
chloroplast &
photop
hosphoryl
-
ation, photosynthesis
5 (Feb.
1
4
-
1
8
)
Enzyme III:
Proposal
Feedback
Presentation /
Pilot Studies
Data Analysis pre
-
lab Part II:
focus on your
enzyme protocol and expected data (due in disc).
Focus on experimental design with team (in disc)
Enzyme proposal feedback presentation (1/team) in
lab
Enzyme Research Proposal
due 48h after
presentation
Enzyme question/methods/schedule (1
/team)
2%
7
%
Metabolic integration
;
DNA & chromosome
structure & replication
EXAM I
Wedne
sday
Feb.
2
3
7:15pm
6 (Feb.
2
1
-
2
5
)
Enzyme IV:
Final Experiment
Focus on Peer Review: our expectations (in disc.)
–
peer review practice exercise
Enzyme proposal returned with comments
Teams collect data (open lab)
Transcription & RNA
processing, translation
& genetic code,
mutation & DNA
repair
7 (
Feb
28
-
Mar
4
)
Molecular
Genetics I: Intro
to
C. elegans
,
heat shock
proteins, and
RNAi / GFP to
study gene
expression
Peer review
conference
Final Enzyme papers in
disc.
Start to introduce Worm unit in disc, observe
wildtype worms
Final Enzyme paper (13%)
+
peer review (2%)
+
author’s response + GEA forms
due in lab or
lecture
View Podcast on worm
morph
(Part 1)
& life
-
cycle,
RNAi, GFP
(Part 2)
before
lab
15%
In
-
class
stations:
C. elegans
gene expression
worksheet
Develop study question and tentative biorationale
Bacterial genetics,
transposable elements
8 (Mar
7
-
1
1
)
Molecular
Genetics II:
Developing
experiments on
gene expression
in
C. elegans
Worm stress
/
gene expression
+ statistics
(ANOVA, Chi
-
square)
Prelab
due in disc.
Worm proposal feedback presentations
in lab
Materials and schedule sheet
-
detailing how, when,
and who will perform each step of experiment
Mid
-
semester evaluations
3
%
Spring Recess March 14
-
18
Genetic engineering,
prokaryotic gene
regulation
9 (Mar
21
-
25)
Molecular
Genetics III:
Worm
experiments
Worm proposal mini
-
poster peer review in
discussion
Worm research proposal
poster
due in lab
Do worm experiments
-
establish treatments
, pilot
studies,
and RNAi set up, gather data
8%
Prokaryotic &
eukaryotic gene
regulation, genomics
10
(Mar.
28
-
Apr
1)
Molecular
Genetics IV:
Data
Analysis
and Poster
Development
Biocore 324 preview in lab (in disc.)
Worm data collection
and poster development
Worm data analysis
(chi
-
square)
: what do your data
indicate?
Worm proposal posters returned with comments
Protein trafficking,
cell
signaling
EXAM II
Tu
esday
Apr.
5
7:15pm
11
(Apr.
4
-
8)
Signal
Transduction I:
Intro to Yeast
System
Do genetic transformations in discussion
,
introduction to cell shape and
B
-
gal assay
Generate tentative question for Sign. Trans project
(1/team) due in
lab
C. elegans
mini
-
posters + GEA
due
in Fri. lecture
15%
G
-
linked receptors,
second messengers,
growth factors &
receptor tyrosine
kinases
12
(Apr.
11
-
15)
Signal
Transduction II:
Present
Proposals (disc
& lab);
Signal Transduct. prelab
due in disc.
Signal Transduction feedback presentation (1/team)
in lab
Intro to 2
-
way ANOVA during presentations
Materials & schedule for signal transduction project
(1/team) due in lab
3%
Ras signaling,
hormones, cytoskeletal
proteins
13
(Apr.
1
8
-
2
2
)
Signal
Transduction III:
Pilot Studies &
Your Experiment
Peer reviewers meet to discuss signal transduction
proposal papers (in disc)
Signal Transduction Proposal Paper (1
3
%) +
peer review (2%)
+ author’s response
–
due
Wed
lecture or
lab
Data collection I in lab
1
5
%
Cell movement, cell
adhesion, cell cycle
14
(Apr.
25
-
29)
Signal
Transduction IV:
Data Analysis
Genetics Concept Assessment in disc
Data collection II in lab
Cell cycle & apoptosis,
cancer
15
(May
2
-
6)
Signal
Transduction V:
Poster Presen.
Practice presentation & group poster peer review
during
discussion
Signal transduction Posters Formal
Presentations (1/team)+ GEA
forms
Final course evaluation
15%
Team work, class participation and check
assignments
3
%
Grade Breakdown
Percentage
Letter Grade
90
-
100
A
80
-
89
B
70
-
79
C
Assignments, due dates, and individual assignment weight in percent are detailed above.
Biocore 304 is a
Comm
-
B course and therefore is writing intensive. Grades on assignments
will not be curved. For those few individuals that are on the borderline at the end of the
semester, we will assign intermediate grades (AB and BC) based on our evaluation of your
part
icipation (in both lab AND discussion), teamwork and your effort.
60
-
69
D
Percentage
Letter Grade
90%
A
80%
B
70%
C
60%
D
Percentage
Letter Grade
90
-
100
A
80
-
89
B
70
-
79
C
60
-
69
D
Enter the password to open this PDF file:
File name:
-
File size:
-
Title:
-
Author:
-
Subject:
-
Keywords:
-
Creation Date:
-
Modification Date:
-
Creator:
-
PDF Producer:
-
PDF Version:
-
Page Count:
-
Preparing document for printing…
0%
Comments 0
Log in to post a comment