Biotechnology Laboratory Methods and
Laboratory operations, management, equipment, instrumentation, quality control techniques, and
Includes laboratory practice in sampling and storage of bio
pH meters, mixing buffers, performing measurements, preparing solutions, and performing
BITC 1211; CHEM 1405 or 1411; MATH 1314;
or instructor’s approval; ENGL 0305 or 0316
AND ENGL 0307 or 0326, OR higher level course (ENGL 1301), OR placement by testing.
The Lone Star College System
is dedicated to providing the least restrictive learning environment for all
students. The college district promotes equity in academic access through the implementation of reasonable
accommodations as required by the Vocational Rehabilitation Act of 197
3, Title V, Section 504 and the
Americans with Disabilities Act of 1990 (ADA) which will enable students with disabilities to participate in
and benefit from all post
secondary educational programs and activities. If you require reasonable
because of a physical, mental, or learning disability, please notify the instructor of this
course within the first 2 weeks of the term.
Students with disabilities who believe that they need accommodations in this course are encouraged to contact the
ility Services Office at 936
7239; located in Building E, Office 103H as soon as possible to better ensure
that such accommodations are implemented in a timely fashion.
Students will demonstrate the preparation
of various solutions
, including buffers, to make
dilutions and to accurately measure and transfer solutions volumetrically.
Students use the appropriate S.I. units in laboratory calculations and solve problems
involving conversions between unit expressions.
will collect data using a pH meter. Students will adjust the pH of various
solutions and determine the capacity of various buffer solutions by titration.
Students will use a spectrophotometer to perform simple spectroscopy experiments.
demonstrate a mastery of the fundamentals of low pressure column
chromatography, including ion exchange and gel filtration, by performing
mixtures utilizing low pressure column chromatography.
Students will employ safe laboratory techniqu
es and demonstrate prudent laboratory
Bldg B Office 22
Posted on my door, on my website and handed out in class. Other times are available by appointment.
If I am not in my office during the posted time, please check the biotechnology lab, room
See attached. The calendar is
subject to c
. I will give you as much notice as possible when
changes occur or are anticipated.
SEMESTER / LOCATION
8:30 to 11:30 Tue and Thurs
Lisa A. Seidman
Basic Laboratory Methods for Biotechnology.
Lisa A. Seidman, Cynthia J Moore,
Second Edition, 2009.
NOTE: The second edition is substantially different from the first
edition. ISBN: 0
Scientific Notebook Co.
All are available in
Safety Glasses or Goggles
these must be worn in the laboratory.
A Lab Coat
Local purchase (approx. $25)
May be ava
ilable in college bookstore or locally
Waterproof Finepoint Marker blue or black ink.
Attendance is mandatory. If you are unable to attend a class occasionally, you are responsible for
the material presented during that session
as well as any activity. Please be sure to jot down the
phone numbers of one or two classmates for assistance in these instances.
Because of the difficulty of making up complex laboratories spanning multiple class periods, it is
very difficult to make up
missed laboratories. However, if documentation of extenuating
circumstances is present, the student will be responsible for inquiring about alternate arrangements
that may be possible.
Participation in class discussions and activities is crucial since thi
s course emphasizes the
development of technical skills, communication skills, the ability to work responsibly in a group,
and the honing of critical thinking skills.
You will be graded for this participation (
. By definition, you must
be in class
You will receive a score of 0 for any activity that takes place during an unexcused
If you have 3 unexcused absences
from the laboratory
you will be
for the safety of the other students.
s you will fail the course.
NO LONGER ATTENDING CLASS DOES
CONSTITUTE WITHDRAWAL FROM THIS
CLASS, NOR DOES A STUDENT’S NOTIFICATION TO THE INSTRUCTOR THAT THE
STUDENT WISHES TO BE DROPPED. FAILURE OF A STUDENT TO FILL OUT A
“SCHEDULE CHANGE FORM” TO O
FFICIALLY DROP THIS CLASS MAY RESULT IN A
GRADE OF “F.”
THE LAST DAY TO DROP THIS CLASS AND RECEIVE “W” IS
AFTER THIS DATE, NO WITHDRAWALS WILL BE ISSUED.
Professional behavior is required in all lectures and laboratories in this course. This includes
observation of safety procedures, courtesy to your peers, respect for the equipment and reagents and
cooperation in group projects. Students will be expected
to maintain appropriate decorum in the
classroom and may be dropped from the class for unruly or disruptive behavior.
Often research is a cooperative effort. Many of our experiments will be performed of groups of two
(rarely three). It is important that
each individual contribute to completing the experiment. Data
obtained as a group is shared but each person in the group is required to record raw data in their lab
notebook and to write up their own lab report in their own words and to produce their own g
tables etc. Individual grades will reflect individual effort.
Counseling is available for academic, career, and personal matters. Contact either:
Phone: (936) 273
Phone: (936) 273
Three Lecture Tests:
100 points each
= 300 points
= 200 points
10 points (x
60 points (x
0 points (x
Note that a grade of “C” or higher is required in BITC courses in order to obtain the AAS
degree or ATC certificate.
UP EXAMS WILL BE GIVEN ONLY FOR AUTHORIZED ABSENSES; WRITTEN
PROOF (FROM A DOCTOR, FOR EXAMPLE) WILL BE REQUIRED.
THERE WILL BE NO MAKE
Late assignments will lose 10% credit. After one week, a late assignment may be turned in only with
permission of the instructor for 1/2 credit.
Test Corrections: For th
exams (but not the Final exam), an opportunity to make test corrections
will be given for partial credit. Details will be given in class.
: No other extra credit will be offered or allowed.
I regard high profession
al standards as one of the most important lessons you can take from this class.
My expectation for this class is that each of you will act in a responsible and ethical manner. Please
take a look at the sections in our course catalog on Academic Integrity
and Appeals (p.
) for details
on your responsibilities as a student.
You as individuals are responsible for completing all assignments in this course. You may consult
with other members of the class on homework, laboratories, projects and exam
exams themselves), but I expect each of you to do your own work.
If you work with another student,
please note whom you worked with on the assignment.
The laboratory reports
are the responsibility of each student. The bac
kground, in particular,
requires that you do background research. Such research must be reported
IN YOUR OWN WORDS
. Inappropriate use of other work or citation will result in a
grade of “0” for the exercise. There will be
If there is reason to believe you are not meeting those standards, you may expect appropriate
disciplinary actions to be taken, as described in the catalog.
Review the safety procedures described in chapter 2 of th
e Laboratory Manual (Seidman and
Moore). You are responsible for learning and observing safety procedures. You must sign and
date a Biotech Laboratory Safety Agreement before you will be allowed to work in the lab. Be
sure that you check the MSDS (copy in
the lab) before using any chemical.
All students must wear a
lab coat and safety goggles or glasses in the laboratory at all times
unless told to do otherwise. If you fail to bring your lab coat and goggles (glasses), you will be
asked to leave the lab
may be stored
in the lab. You may bring a combination lock
to put on one drawer in the lab to store goggles, gloves etc.
Lone Star College System (LSCS) is committed to maintaining the safety of the students,
faculty, staff, and guests while visiting any of our campuses. See
for details. Register at
to receive emergency notifications.
In the event of an emergency, contact LSCS Police at (281) 290
591 or X5911.
THE LABORATORY NOTEBOOK
Our partner companies have emphasize
d repeatedly how important good notebook skills are for their
employees. To help you meet their standards we will follow a specific set of guidelines for keeping
The notebook must be bound, with numbered pages.
You will write only in ink.
You will not remove pages, erase or deface any entries. You can
make changes by using a single strikeout
and dating the change.
3 will be reserved for the Table of Contents.
Before you walk into the lab for an experiment, the following m
ust be in your notebook:
Title of the experiment
Description of the experiment (3
5 sentences per day)
HYPOTHESIS for the experiment.
You may need to be creative, but you can come up
Flow diagram for the experiment, including anticipated time.
All necessary calculations.
Blanks to fill in data to be acquired.
Do not rewrite the protocol.
Refer to the written protocol, as necessary.
NOTE any changes given to you about the laboratory, written or otherwise.
Entry in the Table of Contents.
er any questions or perform any calculations given to you with the protocol
YOU WILL BE GRADED IMMEDIATELY ON THESE ENTRIES
Modifications and safety notices from the instructor will be written down in the notebook.
All data, all observations, changes, p
roblems etc will be made directly in the notebook.
It is not
necessary to rewrite the protocol.
It's only necessary to describe variations from the protocol.
Include details about all reagents, materials and equipment used.
If you are observed writing any
of this information anywhere but your notebook it will cost you
points on the spot.
At the end of EVERY LAB PERIOD, you AND the instructor will sign and date every new
page of data entry. Missing the signing will cost you credit.
page blank at the end of each experiment. Your lab write
up and answers to
assigned questions will be written up SEPARATELY (see below) and turned in for grading.
The returned, graded write
up will be incorporated into the notebook by stapling in onto t
Lab reports are separate from your lab notebook. Lab reports are due one week after each lab is
All lab reports must be generated using a computer and Microsoft Excel and
Microsoft Word; handwritten reports are not a
Computers are available in the
science/math area of the ELC (Extended Learning Center).
Computers are used by all
biotechnology laboratories and companies want people who know how to use them.
There will be TWO laboratory report formats used th
is semester: full format and
You will download the appropriate answer sheet from the course website and fill in the blanks,
including some short answers. No regrades are allowed on these reports.
FULL LAB REPORTS
must be produced using a word
processing program such as
. All graphs should be produced using
and pasted into the Word document or
otherwise incorporated into your report (see below)
Proofread your lab repor
t before you turn it in. Make the necessary corrections. Well
reports will get better grades than poorly written ones. If you have trouble writing scientific
English for any reason, consult with me early in the semester and we will arrange for ap
These reports will be graded in detail, including a writing grade. You will be allowed the
opportunity to correct these reports and turn them in to obtain a better grade.
Lab Report Format:
Describe the problem and what the lab report is about in one to two pages. Give
one sentence is not enough. You will be expected to include one or more
references. Include a copy of your
(from your notebook) with every lab
Materials and Methods:
Include a detailed protocol. Refer to the protocol that you have followed,
and describe all changes.
This is an important section. Raw data does NOT go here. Try to use your data to tell a
story. Use a computer to make
all graphs. Be sure that each figure has an explanatory legend. Use
This is the place for your interpretations and your conclusions. Please
summarize your experiment here. Describe the results and account for accura
cy, precision, errors
State whether your hypothesis was proven or no
t. What could you do to make this a
better lab or continue with the result you obtained? Answer all questions posed.
Raw Data and Calculations:
Put these at the end. Normall
y these would not be part of a lab
report, but they will allow me to help you trap errors if there are any in your write
up. Put the data
into useful tables.
are responsible for cleaning up after yourself. No one likes
leaves a mess! The
biotechnology program is designed to help give you the training to prepare you for the workplace. I
will assume that you are not ready if you do not keep your area clean and neat or if you leave your
glassware in the sink for someone el
se to do.
will be assigned to check various important areas of the lab each class period to make sure
that the areas are completely cleaned and ready for the next class.
Proper maintenance of individual and group areas is a GRADED ACTIVITY in t
GLASSWARE CLEANING I
are responsible for cleaning and rinsing any glassware with distilled water
that you use before you leave the lab.
Rinse all glassware 3
4 times with tap water before placing the glassware in the detergent.
These rinses will serve to remove chemicals so they are not transferred to the detergent and
then potentially to someone’s hands.
Wash all glassware with dete
rgent. Use a brush to help to remove chemicals.
Immediately rinse each piece of glassware 4
5 times with very warm water. Detergent can
cause problems if it is left to dry on the glassware.
Then, rinse the glassware with
stly, place the glassware on the peg board to drain or, if the peg board is full, place on clean
paper towels, upside down (unless it is unstable), next to the sink so the glassware can drain.
Clean up all of the area that you worked in. Wipe u
p any spills as they occur. Be sure that you have
left equipment in the proper state i.e., pH electrodes should be placed in pH 7 buffer, set for standby
and the air hole closed.
Clean any spills around the balance immediately.
place caps on chemical bottles immediately and wash and rinse any spatulas that you
may have used. Dispose of the weighing boats.
These are disposable. They are to be disposed of in the
Pasteur pipets in the sink
to be washed. They break too easily and someone will receive a cut or
Glass Only Box : Do not
put paper in these boxes.
Biohazardous materials : Place in one of the biohazard bags.
KEEP TRACK OF YOUR C
TENTATIVE CLASS SCHE
Assignments in Seidman and Moore, 2
edition (SM)*, Seidman (Seid), or the on
January 17, 19
1) Introduction to a safe workplace.
2) Working Safely in the Laboratory:
clothing, gloves & eye protection
3) Safety Video: Practicing Safe Science
4) Glassware Washing
The Measurement of
Mass and Volume
6) Introduction to Accuracy and Precision
Math quiz, part 1
exponents and scientific notation; the
Ch 13; 19,
Seid: 1, 3
Laboratory orientation: labwa
reagents, care and maintenance.
chemicals and solutions. Glass, paper
biological waste disposal.
Care and use of laboratory balances.
Use of volumetric glassware.
Using a Pasteur pipette
Using a Mohr
10 mL Mohr pipet.
Use of the balances
1) Things you need to know before you
start the first lab:
2) Solution basics; making a solution at a
solution/dilution, part 1
Water in the lab
5) Theory of acids and bases
pH, pH meters and pH electrodes
Acids & Bases Problem Set
Ch 28 (I
Seid: 2, 11,
Calibration and use of the pH meter
calibration of pH meter.
Making up solutions
of strong acids, strong bases, weak
and weak bases
Use of the pH meter
on the use of the
program, Microsoft Excel for making
graphs and inserting them in a
Microsoft Word document.
Extended Learning Center:
Using the directions on pages 9
complete Lab Assignment 1
January 31, February 2
) Buffer basics
2) Mathematics Review: significant
figures, decimals, scientific notation.
3) Significant figures tutorial
ath Quiz: solution/dilution part 2:
Seid 4, 12,
phosphate buffer stock solutions.
Practice with instructor observation.
Extended Learning Center:
Lab Assignment 2
EXAM 1: Feb
) Before you start the third lab:
Basics of using a visible
opulations, samples, variables and
measurements of central tendency and
dispersion (mean, variance, standard
deviation, coefficient of variation and
Ch 23, 25
up, with statistics
How to write up your data and
Observe individual setup & use of
Making a simple dye
solution and visible
1) Protein purification overview, part 1
Mathematics: review dilutions;
applications of Beer’s La
Ch 29, 33
Micropipetting check using the
1) Protein purification overview, part 2
Mathematics: making a purification
table; math quiz: graphing using
Finish up labs (catch
1) The Centrifuge.
2) The Centrifuge Video: Basic
3) Safety Video: Centrifugation Hazards
calculations and use of a nomogram;
theory of dialysis
CH 31, 32
Overview of Protein Purification
saturated ammonium sulfate
Precipitation of rabbit
on of protein peak
by UV spectroscopy.
Writing a scientific hypothesis.
Writing methods and materials
1) Structure of Proteins
; primary structure
(amino acids) and secondary;
) Molarity calculator
4) Video: Column Chromatography.
. Setting up a DEAE
exchange cellulose column
Purification of rabbit
immunoglobulin by ion exchange
Short Lab Write
EXAM 2: MARCH 22
Structure of Proteins
; tertiary and
quaternary structure and function
Mathematics: pH/pKa of amino acids
and zwitterions; the isoelectric point
3) Mathematics: 3
visualization and manipulation
Large Molecule Problem Set
2) Use of animals in the laboratory;
production of monoclonal/polyclonal sera
Mathematics: serial dilutions;
graphing ELISA data
Ch 17, 24
ELISA in the
identification of proteins.
April 3, 5
1) Upstream processing; preparation of
cells and fermentation
Videos: (Fractionating a Cell
3) Mathematics: fermentation yields
Analysis of IgG
using gel electrophoresis.
(Purification of IgG).
Biotechnology and the Regulation of
Medical and Food Products
2) Enzyme kinetics; enzyme reactions
Ch 3, 4, 5
activity and crystallization of
Introduction to Product Quality
Mathematics: dosage of
Ch 6, 8
EXAM 3: APRIL 22
1) Documentation : The Foundation of
2) Quality Systems in the Laboratory
3) Safety Video, OSHA Safety Video,
Safety in the Research Laboratory
(Radionuclide Hazards, Chemical
Hazards, Emergency Respons
Production of a
product under GMP Conditions.
lab report (GMP)
May 1, 3
Clean up and check out.
May 8, 10
May 8, REVIEW; May 10,