2012 Minicourse Syllabus - NIAID/NIH Filariasis Research Reagent ...

fabulousgalaxyBiotechnology

Oct 1, 2013 (4 years and 14 days ago)

94 views

NIH Filariasis Research Reagent Resource Center (FR3)

Experimental Techniques in Filariasis Research


21
-
25 May 2012


University of Georgia, College of Veterinary Medicine


Class Schedule



Instructors:

Andy Moorhead, DVM, MS, PhD


University of Georgia

Steve
n

Williams, PhD


Smith College

Shelly Michalski, MS, PhD


University of Wisconsin
-
Oshkosh

Mike Dzimianski, MS, DVM


University of Georgia

Prasit Supakorndej, BS(Pharm), MS, PhD

University of Georgia

Sue Haynes, BS


Smith College

John McCall, MS, PhD


University of Georgia

Steve Harvey, DVM, MS


University of Georgia

Leanne Alworth, DVM, MS


University of Georgia

Caroline Decker, AB


Smith College

Katy Griffiths, CVT, AB, MS


University of Wisconsin Oshkosh


Support Personnel:

Bob Storey, MS, RVT

Erica

Burkman, MS

LaDonna Allen


Location:


Lectures:

Room 312 in Driftmier Engineering Building, Room 236 in Veterinary Building


Labs:

Room G5 in the Wildlife Health Building


Day

Time

Event


Location


Monday

8:30
-
8:45

Welcome and Introductions 236




8:45
-
9:
15

Lecture

(Moorhead):

FR3 parasite resources available 236




9:15
-
9:30

Lecture

(
William
):

Introduction for molecular labs

236




9:30
-
10:00


Lab
(Dzimianski/Supakorndej)
: Use blood from cat Insectary




with
B. malayi

microfilariae (mf) to set up




membrane
-
feeders

and start infecting mosquitoes.






10:00
-
10:15

Break

G2



10:
15
-
10:50

Lab
(Dzimianski/Supakorndej):

Collect L3 from

G5




mosquitoes, examine L3 under microscope, inject



L3 into jirds.


11:00
-
12:00

Special Seminar S175 Coverdell




12:00
-
12:15

Group Photo




12:15
-
1:30

Lunch




1:30
-
2:30

Lab
(Dzimianski/Supakorndej)
:

Necropsy
B.
G5





malayi

infected jirds;
Collect
L4

and microfilariae





from peritoneal cavity and examine under microscopy.



2:30
-
3:00

Lab
(Haynes/Decker):

Set up PCR Reaction

G5






3:00
-
3:30

Lecture

(Williams):

PCR and Sequencing to Identify 312




Filarioid Nematodes




3:30
-
3:45

Break (Refreshments)

G2




3:45
-
4:30

Lecture

(Michalski):

Overview of nematode

312





biology and introduction to filarioid nematodes.






4:30
-

5:30

Lecture

(Michalski):

Lymphatic Filariasis 312




Research in the Field.





5:30
-
6:30

Lab
(Haynes/Decker):

Load Gel

G5

(Meet in G5)


Tuesday

8:30
-
9:30

Lab
(Dzimianski/Supakorndej)
: Collect adult

G5




worms and microfilariae from peritoneal cavity




and examine under microscopy. Identify and





sort male and female worms.




9:30
-
10:30

Lecture
(Griffiths):

A. viteae

lecture

312



10:30
-
10:45

Break
(Refreshments)

G2



10:45
-
11:15

Lab
(Haynes/Decker):

Set up DNA sequencing reaction

G5



11:15
-
12:15

Lecture

(Williams):

PCR and DNA Sequencing to Study

312



Filarioid Nematodes



12:15
-
1:45

Lunch



1:45
-
4:30

Lab

(Griffiths):

Anti
-
Wolbachia

surface protein

G5
Immunohistochemistry.



4:30
-
5:30

Lecture

(McCall):

B. malayi / B. pahangi

mosquito 236


vector phases.

(Meet in
312
)


Wednesday

8:30
-
9:30

Lecture

(Harvey/Alworth):

Using gerbils in 312




biomedical research
.




9:30
-
10:30

Lecture

(Michalski):

Transcriptome analysis
-

312




Applications and Methods.



10:30
-
10:45

Break (Refreshments)

G2




10:45
-
11:15

Lab Demonstration

(Dzimianski/Supakorndej):

Insectary



Pick mosquito pupae; put mosquito eggs in water




for hatching into larvae.




11:15
-
12:15

Lab
(Haynes/Decker):

Set up qRT
-
PCR

G5






reactions.

.


12:15
-
1:15

Lunch



1:15
-
4:15

Lab
(Michalski):

Dissect freshly fed (2 h
ours prior)
G5



mosquitoes and quantify midgut penetration of
L1.



Dissect day 5.5 and day 9.5 mosquitoes


recover



and examine L2 and L3 stages



4:15
-
5:15

Lecture

(McCall):

B. malayi / B. pahangi


236



vertebrate host(s) phase.



7:00
-
until …

Dinner at
Square One Seafood

(sponsored by Merial).


Thursday

8:30
-
9:00

Lab

(Dzimianski/Supakorndej):

Set up mosquito Insectary




larval cultures.



9:00
-
9:45

Lecture

(Williams):

G
ene Expression 312



Analysis Continued



9:45
-
10:00

Break
(Refreshments)

G2



10:00
-
11:
30

Lecture

(
Williams
):

The global effort

to
312



eliminate lymphatic filariasis.






11
:
45
-
1:30

Lunch



1:30
-
2:30

Lecture

(Williams
): qPCR
and Bioinformatics 312




2:30
-
3:30

Lab
(Dzimianski/Supakorndej):

Necropsy of G5



Brugia
-
infected jirds with lymphatic infection.



3:30
-
3:45

Break (Refreshments)




G2



3:45
-
4:45

Lecture
(McCall)
: Wolbachia 236



4:45
-
5:45

Lecture

(McCall):

D. immitis

mosquito vector

236



phase,
D. immitis

vertebrate host(s) phase.


______________________________________________________________________________






(Location TBA)



Friday

8:30
-
9:30

Lecture

(Williams):

Interpretation of PCR, qPCR, and sequencing
data



9:30
-
10:15

Lecture
(Williams):

Finding clones in cDNA and genomic libraries.
Bioinformatics.






10:15
-
10:30

Break (Refreshments)





10:30
-
11:15
Lecture

(Williams):

Bioinformatics of filarial data sets. Accessing
genomic data.



11:15
-
12:00

Lecture

(Williams):

Using online
bioinformatics resources



12:00
-
1:30

Lunch



1:30
-
4:00

Bioinformatics lab

(Library computer room):

Individual
bioinformatics problems.



4:00
-
4:30

Closing remarks and evaluations