M.Sc. Microbiology - Karpagam University

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Feb 12, 2013 (4 years and 2 months ago)

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Code

Subjects

Ins

Marks

Exam

Hrs

Credit

CIA

ESE

Total

SEMESTER


I

1
1
MBP101

General Microbiology and
Classification


4

40

60

100

3

4

11
MBP102

Microbial P
hysiology and Metabolism

4

40

60

100

3

4

11
MBP103

Molecular genetics

4

40

60

100

3

4

11
MBP104

Bioinstrumentation

4

40

60

100

3

4

11
MBP111

Basic Practical


I

6

40

60

100

9

3

11
MBP112

Basic Practical


II

6

40

60

100

9

3

11
NMP101

Man and Microbes*

2

40

60

100

3

2

Semester total

30

280

420

700


24

SEMESTER


II

11
MBP201

Virology

4

40

60

100

3

4

11
MBP202

Medical Microbiology

4

40

60

100

3

4

11
MBP203

Microbial Technology and
Intellectual Property Rights

4

40

60

100

3

4

11
MBP204

Environmental and A
gricultural
Microbiology

4

40

60

100

3

4

11
MBP211

Advanced Practical


III

6

40

60

100

9

3

11
MBP212

Advanced Practical


IV

6

40

60

100

9

3

1
1
NMP201

Mushroom Cultivation*

2

40

60

100

3

2

Semester total

30

280

420

700


24

SEMESTER


III

11
MBP301

Advan
ced Immunology

4

40

60

100

3

4

11
MBP302

Food and Industrial Microbiology

4

40

60

100

3

4

11
MBP303

Genomics and Proteomics

4

40

60

100

3

4

11
MBP304

Biostatistics and Research
Methodology

4

40

60

100

3

4

11
MBP305

Cell
B
iology
#

-

-

100

100

3

3

11
MBP311

Application Oriented Practical


V

6

40

60

100

9

3

11
MBP312

Application Oriented Practical


VI

6

40

60

100

9

3

11
MBP381

Industrial Training

-

100

-

100

-

3

11
NMP301

Laboratory Animal Care
*

2

40

60

100

3

2

Semester total

30

380

520

900


30

SEMESTER


IV

11
MBP491

Project and Viva Voce

-

80

120

200

-

12

Semester total

-

80

120

200

-

12

Course total

90

1020

1480

2500


90

* Non
-
major Elective subjects for other department students.

# Self
-
study paper

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2

Semester


I

1
1
MBP101


GENERAL MICROBIOLOGY AND CLA
SSIFICATION








4H


4C


SCOPE

Microorganisms play a vital role for the welfare of human. These microbes had to be
manipulated for such benefits. Thus the need arises for the study of microbial techniques
for well exploitation of microbial population.
The basic task of microbial taxonomy is to
differentiate microorganisms. It enables the students to identify any microorganisms.


OBJECTIVE

After studying this paper student could be able to:

* define the science of microbiology and describe some of the g
eneral methods used in the
study of microorganisms

* discuss the fundamental similarities and differences between microorganisms based on
their phenetic and phylogenetic relationships.

* explain the taxonomy and classification of bacteria, fungi, algae, v
irus and protozoa.


UNIT


I

History of Microbiology
-

Milestones of progress. Microbial evolution and Diversity


Taxonomic ranks
-

Classification system


Phenetic and Phylogenetic


Major
characteristics


Classical, Molecular


Assessing microbial phy
logeny
-

The Major
divisions of Life.


UNIT


II

Microscopy


Lenses and the bending of light
-

Microbial resolution
-

Simple
-

compound,
dark
-
field, phase contrast


interference, normaski, fluorescent and electron microscopes
-

SEM, TEM, freeze fraction con
focal microscopy and their applications.


UNIT


III

Classification of bacteria
-

Bergey’s manual and its importance
-

Eukaryotic algae


classification of algae, growth and reproduction of chlamydomonas, volvox,

diatoms,

Red
algae
and

brown algae. Classif
ication of Virus


DNA, RNA viruses.


UNIT


IV

Principles Methods of sterilization. Physical methods
-

Chemical method. Staining
technique
-

Simple staining
-

Differential staining.

Culture technique
-

Maintenance and
Preservation of cultures. Microbial cultu
re collection centers.


UNIT


V

Classification and Taxonomy of fungi


Alexopolous


Distribution


general
characteristics of divisions


Economical importance of Fungi. Classification, nutrition,
reproduction, characteristics and importance of protozoa


Entamoeba histolytica
,
Giardia,
Trichomonas, Plasmodium
.



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TEXT BOOKS

1.

Powar, C.B. and H. F. Daginawala, 2003. General Microbiology. Volume: II, Himalaya
Publishers, New Delhi.

2.

Singh, R.P., 2007.
General Microbiology
. Kalyani Publishers, New Delhi.

3.

Frobis
her, H., Hinsdil, R.D., Crabtree, K.T. and Goodhert, D.R. 2005.
Fundamentals of
Microbiology
, Saunder and Company, London.


REFERENCES

1.

Alcamo, E.I., 2001. Fundamentals of Microbiology. 5
th

Edition, Benjamin / Cummings
Publishing Co., California.

2.

Holt, J.G.
, N.R. Krieg, H.A. Peter, S. James, T. Staley and S.T. Williams, 2000.
Bergey’s Manual of Determinative Bacteriology. 9
th

Edition, Lippincott Williams and
Wilkins Publishers. Baltimore.

3.

Pelczar, Jr. M.J., E.C.S. Chan and N.R. Kreig, 2003. Microbiology 5
th

Edition Tata
McGraw
-
Hill Publishing Company. New Delhi.

4.

Prescott, M., J.P. Harley and D.A. Klein, 2007. Microbiology. 7
th

Edition, McGraw
-
Hill Inc. New York

5.

Salle, A.J., 2007. Fundamental Principles of Bacteriology 6
th

Edition, McGraw Hill
Book Company, I
nc. N.Y, London.

6.

Stanier, R.Y., J.L. Ingraham, M.L.
Wheelis and P.R. Painter, 2007. General
Microbiology. Macmillan Press Ltd. London.

7.

Talaro K. P. & Talaro A. (2006).
Foundations in Microbiology. McGraw
-
Hill College
Dimensi.


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4

Semester


I

1
1
MBP102


MIC
ROBIAL PHYSIOLOGY AND METABOLISM

4H

4C


SCOPE

Activities of microorganisms are very important to almost every sector of concern to
mankind. This paper provides information about all the microbial cellular functions and
various metabolic pathways in microb
es. The students will be able to understand and
predict the intermediate metabolism of any microbe used in Industrial production processes


OBJECTIVE

o

To gain the knowledge with the various inner and outer structures of prokaryotes and
eukaryotes in detail.

o

To impart knowledge on metabolic function and biochemical reaction going on inside
the microbial cell

o

To teach metabolic pathways, their regulation and engineering, and methods used in
their elucidation.


o

To teach students about cell cycle, growth and met
hods to determine microbial growth.

UNIT


I

Microbial cell


Ultra
-
structure of Prokaryotic and Eukaryotic cell


Sub
-
cellular structures
and cell envelope, Slime, Capsules, cell wall, pili, flagella, Cell inclusions, Endospores


Sporogenesis, Cell mem
brane


liposomes.


UNIT


II

Microbial nutrition


common nutrient requirements, growth factors


Uptake of nutrients
by cell
-

Nutritional groups of microorganisms. Growth curve


Measurement of microbial
growth


growth kinetics


Batch, Continuous an
d Synchronous cultures. Influence of
environmental factors on growth.


UNIT


III

Carbohydrate metabolism


EMP, HMP and ED pathway


TCA cycle


Glyoxylate cycle


Aerobic respiration and Anaerobic respiration. Generation of energy
-

substrate level and
oxidative phosphorylation


ATP generation. Lipid catabolism
-

beta
-
oxidation,
Fermentation.


UNIT


IV

Biosynthesis of fatty acids, nucleotide, amino acids, Phospholipids, Archaeal lipids. Cell
wall biosynthesis of gram positive and gram negative bacteri
a. Microbial pathogenesis
-

Toxins
-

characterization, mechanism of action.


UNIT


V

Photosynthesis


Oxygenic and Anoxygenic


The prototrophic prokaryotes
-

Purple
photosynthetic bacteria,
sulfur

oxidizing & reducing

bacteria


Methanogenesis


assimilati
on of carbondioxide


photosynthetic pigments, Bioluminescence.


TEXT BOOKS

1.

Doelle, H.W., 2005. Bacterial Metabolism. Elsevier India Pvt. Ltd., New Delhi.

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5

2.

Berg JM, Tymoczko JL, Stryer L&Clarke ND 2001. Biochemistry. 5th Ed. WH
Freeman & Co.

3.

Moat AG and Fos
ter J W 2003. Microbial Physiology. John Wiley and Sons, New
York.

4.

Nelson D and Cox MM. 2009. Principles of Biochemistry. W.H. Freeman and
Company, New York.


REFERENCES

1.

Atlas, R.M., 1997.
Principles of Microbiology. 2
nd

Edition.
Wm. C. Brown Publishers,
Lowa, US

2.

Caldwell, D.R., 2008. Microbial Physiology and Metabolism. Second edition, Wm C
Brown Publishers, England.

3.

Madigan, M.T., J.M. Martinko and J. Parker, 2003. Brock Biology of Microorganisms.
10
th

Edition. Prentice Hall. New Jersey.

4.

Rose, A.H., 2008
. Chemical Microbiology


An Introduction to Microbial Physiology.
International Edition, Plenum Publishing Corporation.

5.

White, D., 2003. Physiology and Biochemistry of Prokaryotes. 2
nd

Edition. Oxford
University Press. NY.

6.

Voet D and Voet JG. 2003. Bioche
mistry. John Wiley and sons New York.




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6

Semester


I

1
1
MBP103



MOLECULAR GENETICS


4H


4C


SCOPE

The application of molecular genetics has completely transformed the biotechnology
industry, with new possibilities ranging from the treatment of human

diseases to the
development of new forms of crops. Molecular genetics looks set to be the most promising
and exciting science of the next few decades.


OBJECTIVE

The emergence of molecular genetics has revolutionized large areas of modern biological
and b
iochemical research work and has had a huge impact on the biotechnology industry.
Molecular genetics is concerned with the development of biochemical and genetic
techniques for handling the complex nucleic acids that constitute genetic material.


UNIT


I

Genetics


Historical introduction


Mendelian principles


Nucleic acid as Genetic
information carriers: Experimental evidence


The duplex DNA


chemical & physical
structure of DNA


circular and superhelical DNA
-
Different forms of DNA. DNA
replication



Enzymology of DNA replication
-
Different Modes, Models & Types of DNA
replication
-
Eukaryotic DNA replication.


UNIT


II

Inhibitors of DNA replication
-

Mutagen
-

Mutation


Mutagenesis
-

Luria Delbruck
experiments and its significance

Molecular basis of

mutation

spontaneous and induced
mutations
-

Different types of mutation
-

Mutant detection
-

Mutant selection


Carcinogenecity testing. DNA damage
-
Types of damage (deamination, oxidative damage,
alkylation, Pyrimidine dimers)
-

DNA Repair mechanism (Base

excision, Nucleotide
excision, Recombination repair, SOS repair).


UNIT


III

Genetic code
-

DNA transcription in Prokaryotes and Eukaryotes: General
principles,(Initiation, Elongation and Termination), Inhibitors of RNA synthesis,
Trancriptional control
and modification system


RNA translation in Prokaryotes and
Eukaryotes: General principles, (Initiation, Elongation and Termination)


Polypeptide
synthesis (Maturation and Processing of RNA)
-
Translational modification
-

Regulation of
Gene expression


Ope
ron model (Lac, Trp, Ara)
-

Regulation of Gene expression in
Eukaryotes.


UNIT


IV

Genetic recombination in bacteria


Conjugation, Transformation, Transduction,
cotransduction, Abortive transduction, Sexduction. Linkage and genetic mapping. Phage
genetic
s (Replication cycle)


Phage T4 mutants (Detection and Isolation)


Genetic
recombination


Genetic map of T4 phage, Gene mapping
-
Deletion mapping and
Complementation.


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7

UNIT


V


Yeast genetics
-

Life cycle
-

Metabolism


Genomes
-

Extra chromosomal element
-

Genetic nomenclature
-

tetrad analysis
-

Petite mutants (Mutant isolation and
Complementation)
-
Gene conversio
n and Gene mapping in Yeast. Genetic mapping in
Neurospora
. Genetic mapping in
Drosophila
.






TEXTBOOKS

1.

Freifelder, D., 2002. Molecular Biology
. Narose Publishing House. New Delhi.

2.

Verma, P. S. and Agarwal, V. K.,2008 :
Cell Biology, Genetics, Molecular Biology and
Evolution.

S. Chand & Company Ltd, New Delhi

3.

Gardner, E.J., M.J. Simmons and D.P. Snustad, 2001. Principles of Genetics. 8
th

Edi
tion. John Wiley and Sons, NY.

4.

Guthrie, C. and G. Fink, 2002. Guide to Yeast Genetics and Molecular Cell Biology.
Elsevier Publication, USA.

5.

Klug, W.S. and M.R. Cummings, 2001. Essentials of Genetics. 4
th

Edition Prentice
Hall, New Jersey.

6.

Maloy, S.R.,
J.E. Cronan Jr and D. Freifelder, 2001. Microbial Genetics. Narosa
Publishing House. New Delhi.

7.

Weaver, R.F., 2002. Molecular Biology. 2
nd

Edition. McGraw
-
Hill, New York.


REFERENCES

1.

Tamarin, R.H., 2001. Principles of Genetics. 7
th

Edition. Wm. C. Brown
Publishers.
England

2.

Snustad & Simmons, Principles of Genetics, 4th Edition, Wiley, 2005.

3.

Griffiths et al, 2002. Modern genetic analysis, 2nd Edition, Freeman.

4.

Hartl and Jones, 1998. Genetics
-
Principles and Analysis, 4th Edition, Jones & Bartlett.

5.

Nelson
et al, 2006.Lehninger’s Principles of Biochemistry, 4th Edition, McMillan.

6.

Alberts et al, 2007.Molecular Biology of The Cell, 2nd Edition , Garland.

7.

Lewin, 2007 Genes IX, 9th Edition , Jones & Bartlett.

8.

Watson, J.D., and Baker, T.A., Bell, S.P. Gannm, A. a
nd Levine, M. 2004.
MolecularBiology of Genes. 5
th

Edition, Pearson Education.

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,


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2
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1
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.


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U
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r
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i
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,
,


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b
a
a
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o
r
r
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e
,
,


T
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N
,
,


I
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d
d
i
i
a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


8


Semester


I

1
1
MBP104





BIOINSTRUMENTATION


4
H


4C


S
COPE

This course will explore both theoretical and practical concepts of bioinstrumentation and
its application in a
variety of scientific disciplines. Analytical experiments will be
performed in the laboratory portion of the class.


OBJECTIVES

This course highlights the basic laboratory skills that are essential for beginning
-
level
employment in clinical, pharmaceutica
l, microbiology, biochemistry and biotechnology
laboratories. Upon successful completion of this course, students are expected to be able to
explain bioinstrumentation techniques, design and application.


UNIT


I

Spectroscopy


Properties of electromagne
tic radiations, Instrumentation and
applications of


UV Visible light spectroscopy, Spectrofluorimeter, atomic
spectroscopy, NMR spectroscopy and MALDI

TOF.


UNIT


II


Centrifugation


Principle, types of centrifuges, Principles and applications of
anal
ytical and preparative centrifuge, density gradient and ultra centrifuge. Relative
molecular mass determination and sedimentation coefficient. Sub
-
cellular Fractionation
of cellular components.


UNIT


III


Chromatography: Principle, instrumentation and ap
plications of Ion exchange
chromatography, affinity, gel filtration and column chromatography. Low pressure
liquid chromatography (LPLC) and High performance liquid chromatography (HPLC)
and Fast protein liquid chromatography (FPLC), Gas
-
liquid chromatogra
phy mass
spectroscopy (GC


MS).


UNIT


IV


Electrophoresis: Principle, instrumentation and applications of agarose gel
electrophoresis, sodium doecyl sulphate


polyacrylamide gel electrophoresis (SDS
-
PAGE), native PAGE, isoelectric focusing, immuno elec
trophoresis, Pulse field gel
electrophoresis, capillary electrophoresis, gel documentation


Applications.


UNIT


V

Radioisotopic techniques


introduction, nature of radio activity, types and rate of radio
active decay, units of radio activity, detection

and measurement of radio activity.
Principle, instrument and applications of Geiger
-
Muller counter, solid and liquid
scintillation counter and autoradiography. Biosafety methods in Radio active
laboratory.


REFERENCES

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,


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o
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g
y
y
,
,


2
2
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0
1
1
1
1
.
.


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m


U
U
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r
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s
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i
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y
y
,
,


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o
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e
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,


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,


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a






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4
1
1


0
0
2
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.
.


9

1.

Boyer, R., 2000. Modern Experimental
Biochemistry. 3
rd

Edition. Addison Wesley
Longman. New Delhi.

2.

Chatwal, G.R. and S. K. Anand, 2003. Instrumental Methods of Chemical Analysis.
5th Edition, Himalaya Publishing House, Mumbai

3.

Friedfelder, D., 2001. Physical Biochemistry. 5
th

Edition Oxford P
ublishers. New
York.

4.

Sharma, B.K., 2004.Instrumental Methods of Chemical Analysis, 24
th

Edition, Goel
Publishing House, Meerut.

5.

Wilson, K and J. Walker, 2006. Principles and Techniques of Biochemistry and
Molecular Biology, 6
th

Low Price Edition, Cambridge

University Press, India.

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,


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,
,


2
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.


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U
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,
,


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,


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,


I
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i
a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


10

Semester


I

1
1
MBP111




BASIC PRACTICAL


I



6H


3C


1.

Micrometry

2.

Measurement of pH

3.

Staining techniques: Simple, Gram, Negative and Endospore

4.

Motility determination
-

Hanging drop and SIM inoculation

5.

Cultivation of anaerobic m
icroorganisms


Wrights tube


Mc Intosh anaerobic jar
-

methods.

6.

Permanent Slide preparation

7.

Lactophenol cotton blue mounting of fungi
-

Aspergillus
sp
, Mucor
sp
, Rhizopus
sp
,
Fusarium
sp
, Penicillium
sp

8.

Measurement of microbial growth


Viable count


Direct count


Turbidity methods

9.

Biochemical characterization

a)

Indole

b)

MR

c)

VP

d)

Citrate utilization tests

e)

TSI test

f)

Catalase

g)

Oxidase

h)

Urease

i)

Nitrate

j)

Hydrogen sulphide production test

k)

Litmus milk reduction test

l)

Carbohydrate fermentation tests

m)

Amino acid utilizati
on

n)

Hydrolysis of polymers

Starch utilization

Lipid hydrolysis

Casein hydrolysis

Gelatin liquefaction


REFERENCES

1.

Aneja, K.R., 2001. Experiments in Microbiology, Plant Pathology, Tissue Culture and
Mushroom Production Technology, 3
rd

Edition, New Age Intern
ational (P) Limited
Publishers, New Delhi.

2.

Cappucino, J.G. and N. Sherman, 2001. Microbiology A Laboratory Manual,
6
th

Edition
, Benjamin Cummings, New York.

3.

Dubey, R.C. and D.K. Maheshwari, 2002. Practical Microbiology,
1
st

Edition
, S.
Chand and Company L
td, New Delhi.

4.

Gunasekaran, P., 1996. Lab Manual in Microbiology, 1
st

Edition, New Age
International P Ltd, Publishers , New Delhi.

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,


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o
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y
,
,


2
2
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1
1
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1
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.


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U
U
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,
,


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,


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a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


11

Semester


I

1
1
MBP112




BASIC PRACTICAL


II



6H


3C


1.

Spontaneous Mutation


gradient plate technique

2.

Induced Mutagen
esis
-
chemical and physical
-

UV

3.

Replica plating technique.

4.

Isolation of Mutants & Revertants

5.

Transformation in Bacteria

6.

Bacterial Conjugation

7.

Induction of lac Operon

8.

Spectrophotometric estimation of protein


BSA

9.

Analysis of amino acid by Paper chromatogra
phy

10.

Analysis of amino acid by Thin layer chromatography

11.

Analysis of amino acid by HPLC


REFERENCES

1.

Arora, B. and D.R. Arora, 2007. Practical Microbiology, 1
st

Edition, CBS Publishers
and Distributors, Bangalore.

2.

Benson, H.J., 1998. Microbiological Applicat
ion (Laboratory Manual in General
Microbiology), 7
th

Edition, WCB.

3.

Palanivelu, P., 2004. Analytical Biochemistry and Separation Techniques, 3
rd

Edition,
Twenty First Century Publication, Madurai.

4.

Chakraborthy, P. and N.K. Pal, 2008. Manual of Practical Mi
crobiology and
Parasitology, New Central Book Agency (P) Ltd, India.

5.

Gaud, R.S. and G.D. Gupta, 1999. Practical Microbiology, 1
st

Edition, Nirali
Prakashan, Pune.

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,


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,
,


2
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1
1
1
1
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.


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U
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,
,


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,


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,


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a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


12

Semester
-

I

1
1
NMP101



MAN AND MICROBES



2H


2C


M
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,


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,
,


2
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1
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.
.


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m


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U
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,
,


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,


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,


I
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6
6
4
4
1
1


0
0
2
2
1
1
.
.


13

Sem
ester


II

1
1
MBP201




VIROLOGY




4H


4C


SCOPE

Virologist are highly demanded in the medical research companies, Pharmaceutical
companies, governmental agencies, laboratory testing companies, or cancer treatment or
research companies depending upon the specialization


OBJECTIVE

Virolog
y, often considered a part of microbiology or of pathology, is the study of
biological viruses and virus like agents: their structure, classification and evolution, their
ways to infect and exploit cells of virus reproduction, the disease they cause, the t
echniques
to isolate and culture them and their potential uses in research and therapy.


UNIT


I

Historical perspective of virology
-

Scope of virology
-
Viral classification and properties of
viruses
-

cultivation of viruses (animal inoculation, Embroyana
ted egg and tissue culture)
-

properties of viroids and Prions.


UNIT


II

Morphology, replication, pathogenesis and laboratory diagnosis of Pox virus, Adeno virus,
Herpes simplex virus, EB virus, HIV virus
-

Oncogenic viruses
-

Papova virus, Retro virus
-

o
ncogenes and Oncogenesis.


UNIT


III

Morphology, replication, pathogenesis and laboratory diagnosis of Poliovirus, Hepatitis
viruses


type A, B and D, Rabies virus, influenza virus, mumps virus, Measles virus and
rubella virus.


UNIT


IV

Plant viruses



RNA viruses


TMV, cowpea mosaic virus, Bromomosaic viruses, Satellite
viruses


Double stranded DNA viruses
-

CaMV


Single stranded DNA viruses


Gemini
virus. Structure and replication of Bacteriophage (T4)


Filamentous phage (ΦX174).


UNIT


V

Viral Vaccines and interferons
-

Antiviral drugs
-

Strategies to develop AIDS vaccines
-

Rabies vaccines preparation (animal and cell culture) and their immunization dosage.


TEXTBOOKS

1.

Ananthanarayanan, R. and C.K.J.

Panicker, 2005. Text book of Microbiology. 7
th

Edition. Orient Longman. New Delhi.

2.

Chakraborty, P., 2003. A Text book of Microbiology. 2
nd

Edition. New Central Book
Agency (P) Ltd., Calcutta.

3.

Dubey, R.C. and D.K. Maheswari, 2004. A Text book of Microbiol
ogy. 1
st

Edition, S.
Chand and Company Ltd, New Delhi.

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,


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,
,


2
2
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1
1
1
.
.


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m


U
U
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e
r
r
s
s
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i
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t
y
y
,
,


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C
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m
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b
a
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e
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,


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,
,


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6
6
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4
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1


0
0
2
2
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1
.
.


14

4.

Pelczar, Jr. M.J., E.C.S. Chan and K. R. Kreig, 2003. Microbiology 5
th

Edition Tata
McGraw
-
Hill Publishing Company. New Delhi.

5.

Carter J & Saunders V. 2007.
Virology: Principles and Applications
. 1
st
E
d. Wiley.


REFERENCES

1.

Dimmock, N.J., A.J. Easton and K.N. Leppard, 2007. Introduction to Modern Virology,
6
th

Edition, Blackwell Scientific Publications, Oxford, UK.

2.

Jawetz, E., J.L. Melnic and E.A. Adelberg, 2001. Review of Medical Microbiology.
22
nd

Ed
ition. Lange Medical Publishers, NY.

3.

Prescott, M., J.P. Harley and D.A. Klein, 2007. Microbiology. 7
th

Edition, McGraw
-
Hill Inc. New York.

4.

Stanier, R.Y., J.L. Ingraham, M.L. Wheelis and P.R. Painter, 2007.
General

Microbiology, Macmillan Press Ltd. London.


5.

Flint, S. J.,V. R. Racaniello, L. W. Enquist, V. R. Rancaniello, and A. M.
Skalka.,2003.Principles of Virology: Molecular Biology, Pathogenesis, and Control of
Animal Viruses. American Society Microbiology.

6.

Acheson NH. 2006.
Fundamentals of Molecular Vir
ology
. Wiley.

7.

Knipe DM, Howley PM, Griffin DE. 2006.
Fields Virology
. 5th Ed. Vols. I,II.
Lippincott, Williams & Wilkins.

8.

A.J. Cann,2005.
Principles of Molecular Virology,
Academic Press.

9.

White
,
D. O.
,
Fenner
, F. J. 1994.
Medical Virology
, 4th
edn
.
Academi
c Press
, New
York.

10.

Jay A. Levy
,
Heinz Fraenkel
-
Conrat
, and Oliver S. Owens.,

1994.
Virology .3rd Edition

Benjamin Cummings.


M
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,


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g
y
y
,
,


2
2
0
0
1
1
1
1
.
.


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a
a
g
g
a
a
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m


U
U
n
n
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v
e
e
r
r
s
s
i
i
t
t
y
y
,
,


C
C
o
o
i
i
m
m
b
b
a
a
t
t
o
o
r
r
e
e
,
,


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T
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N
,
,


I
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d
d
i
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a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


15

Semester
-

II

1
1
MBP202



MEDICAL MICROB
IOLOGY


4H


4C


SCOPE

Aimed to incorporate the knowledge about medically important pathogens and their
pathogenicity.


OBJECTIVES

Medical Microbiology introduces basic principles and then applies clinical relevance of
many etiological agents responsible
for global infectious diseases. The infectious disease
cycle of the pathogens enables to solve the epidemics. The territory covered by infections
and the immune response expands each year; we focus on pathogenic mechanisms in order
to foster a student’s ab
ility to solve problems in their future clinical career.


UNIT


I

Laboratory precaution and guidelines


collection


transportation


handling and
examination of pathological specimens


methods of isolation, identification and
interpretation of pathogen
ic organisms


Antibiotic susceptibility testing. Infections


types


methods


Infectious disease cycle. Quality control in microbiology lab and automation
in medical microbiology.


UNIT


II

Morphology, cultural characteristics, antigenic property, pat
hogenecity, laboratory
diagnosis and Treatment


Gram positive organisms


Staphylococcus
sp.,

Streptococcus
sp.,

Bacillus
sp
., Corynebacterium
sp.,

Clostridium sp.
and
Mycobacterium
sp
.


UNIT


III

Morphology, cultural characteristics, antigenic property
, pathogenecity, laboratory
diagnosis and Treatment


Gram negative organisms


E.coli, Klebsiella sp., Proteus sp.,
Pseudomonas sp., Vibrio sp., Salmonella sp., Shigella sp., Treponema sp., Neisseria sp. and
Haemophilus sp.


UNIT


IV

Superficial mycosis

-

Pityriasis versicolor, Tinea nigra, piedra. Cutaneous mycosis
Dermatophytes. Systemic mycosis
-

Coccidiomycosis
-

Blastomycosis


Histoplasmosis.
Opportunistic mycosis
-

candidosis
-

Aspergillosis


Zygomycosis. Subcutaneous mycosis
-

Sporotrichosis
-

C
hromoblastomycosis


Mycetoma.


UNIT


V

Protozoan infections

-

Entamoeba histolytica, Plasmodium vivax, Plasmodium falciparum,
Giardia intestinalis Trichomonas vaginalis, Taenia solium.
Trematodes

-

Fasciola
hepatica, Schistosoma haematobium,
Nematodes

-

Trichuris trichiura, Ascaris
lumbricoides, Wuchereria Bancrofti.




M
M
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r


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o
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c
e
e
,
,


M
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c
c
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16

REFERENCES

1.

Ananthanarayanan, R. and C.K.J. Panicker, 2005. Text Book of Microbiology 7
th

Edition. Orient Longman, New Delhi.

2.

Brook,G.F., J. S. Butel, A. Stephen and Morse, 2003. Medical

Microbiology, 22
nd

Edition. Mc Graw Hill.

3.

Chakraborty, P., 2003. A Text book of Microbiology. 2
nd

Edition. New Central Book
Agency (P) Ltd., Calcutta.

4.

Chander, J., 2002. A Text book of Medical Mycology. Interprint Mehta Publishers,
New Delhi.

5.

Chatterjee,
K.D., 1980. Parasitology in relation to medicine. 12
th

Edition, Chatterjee
Medical Publishers, Calcutta.

6.

Chunin, J., 2000. Parasitology. New York Publishers, London.

7.

Dismukes, W.E., P.G. Pappas and D. Sobel, 2003. Clinical Mycology. Oxford
University Pres
s, UK.

8.

Jawetz, E., J.L. Melnic and E.A. Adelberg, 2001. Review of Medical Microbiology.
22
nd

Edition. Lange Medical Publishers. New York.

9.

Mehrotra, R.S. and K.R. Aneja, 2007. Introduction to Mycology. New Age
International Ltd. New Delhi.

10.

Panjarathinam,
R., 2007. Text book of Medical Parasitology, 2
nd

Edition. Orient
Longman Publishers. New Delhi.

11.

Parija, S.C., 2000. A Text book of Medical Parasitology .All India Publishers and
Distributors, New Delhi.

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17











Semester


II

1
1
MBP203

MICROBIAL TECHNOLO
GY AND


INTELLECTUAL PROPERTY RIGHTS

4H


4C


SCOPE

Microbial technology is concerned with the industrial processing of materials by
microorganisms to provide desirable products or serve other useful purposes. This paper
emphasizes the application

of biological systems to the manufacturing and service
industries or the use of biological processes within the framework of technical operations
and industrial production. It
creates awareness on the Intellectual property rights and
patenting of biotechn
ological processes.


OBJECTIVE

To learn the basic tools in recombinant technology

To understand the various concepts of cloning vectors

To learn the cloning strategies

To familiarize the students, with the principles of bioethical concepts

To emphasiz
e on IPR issues and need for knowledge in patents in biotechnology


UNIT


I

Introduction to microbial technology, Restriction enzymes


nomenclature


types


and its
properties, Isolation of DNA, plasmids and RNA, Handling and quantification of nucleic
acids, radiolabelling and non radiolabelling of nucleic acids, Gel electrophoresis
-

Blotting
techniques.


UNIT


II

Plasmid as Cloning Vectors
-

pBR322, Bacteriophage
-


, M13; Yeast vector. Prokaryotic
hosts:
E.coli
, Eukaryotic hosts: Yeast cell. Gene c
loning
-

basic steps, cloning construction
of cDNA, selection and screening method of recombinants.


UNIT


III

Methodology, development of herbicide resistance plants, delayed fruit ripening,

Biocontrol agents
-

Insecticidal toxin of BT, cry gene and bac
ulovirus.
Methodology,
development and use of transgenic animals, transgenic mice


its application.

DNA
diagnostic in medical forensics.

Biosafety and Bioethics.



UNIT


IV

Patenting


fundamental requirements


patenting multicellular organisms


paten
ting and
fundamental research. Patenting of biological materials, Product patents, conditions for
patenting, Patenting of liveforms,
regulating

recombinant technology, Food and food
ingredients.


UNIT


V

Discrepancies in biotechnology / chemical patentin
g. IPR


historical perspective


recent
developments


IPR in India, IPR and the rights of farmers in developing countries.


M
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.


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,


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18

TEXT BOOKS

1.

Sathyanarayana, U., 2005. Biotechnology. 1
st

Edition. Books and Allied (P) Ltd.,
Kolkata, India.

2.

Dubey, R.C., 2002.
Tex
t book of Biotechnology. S. Chand and Company Ltd., New
Delhi.

3.

Ramawat, K.G., 2003. Text book of Plant Biotechnology. S. Chand and Company Ltd.
New Delhi.

4.

Watson, J.D., M. Gilman and J. Wikowski, 2001. Recombinant DNA. 2
nd

Edition,
Scientific American Boo
ks. W.H. Freeman and Co. NY.

5.

Verma, A. and Podila, G.K. 2005. Biotechnological Applications of Microbes.

I.K.
International Publishing House. New Delhi.

REFERENCES

1.

Brown, T.A., 2001. Gene Cloning and DNA analysis: An Introduction. 4
th

Edition.
Blackwell P
ublishing, USA.

2.

Glick, B.K. and J.J. Pasternak, 2003. Molecular Biotechnology. Principles and
Applications of Recombinant DNA. 3
rd

Edition. ASM Press, Washington.

3.

Old, R.M. and S.B. Primrose, 2003. Principles of Gene Manipulation. 6
th

Edition.
Blackwell
Scientific Publication. London.

4.

Primrose, S.B., 2001. Molecular Biotechnology 2
nd

Edition. Blackwell Scientific
Publishers, Oxford Press, London.

5.

Winnacker, E.L., 2003. From Genes to Clones: Introduction to Gene Technology. 1
st

Edition, VCH. Weinhein. Ger
many.

6.

Slater, A. and N. Scott, 2003. Plant Biotechnology
-

The Genetic Manipulations of
plants. 2
nd

Edition, Oxford University Press. New York.


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,


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19

Semester
-

II

11
MBP204

ENVIRONMENTAL AND AGRICULTURAL
MICROBIOLOGY





4H


4C


SCOPE

This course has been de
signed to provide the student insights into these invaluable areas of
Environmental microbiology, which play a crucial role in determining its future use and
applications in environmental management. Provides detailed idea about biofertilizer
production a
nd plant disease.


OBJECTIVES

To educate the students about concepts of designs of water distribution systems, sewer
networks, working principles and design of various physical, chemical and biological
treatment systems of water and waste water. To study a
bout the biofertilizers, plant disease
and

increasing soil fertility.


UNIT


I

Aquatic environment
-

microbiology of water
-

water pollution and water borne pathogens.
Bacteriological examination of water, indicator organism. Microbiology of sewage.
Che
mical and biochemical characteristic of sewage. methods of sewage treatment
-
physical
screening, chemical, Biological (sludge digestion; activated sludge, aerating filters,
oxidation pond).


UNIT


II

Microbiology of air, Microbial contaminants of air, so
urces of contamination, Microbial
indicators of air pollution. Enumeration of bacteria in air. Air samplers & Sampling
techniques. Air sanitation.


UNIT


III

Bioremediation


Contaminated soil


aquifers


Marine pollutants


Air Pollutants,
stimulation
of oil spills degradation. Bioremediation of air pollutants. Bioleaching


recovery of metal from ores


oxidation of minerals


testing for biodegradability.


UNIT


IV

Biological Nitrogen fixation
-

symbiotic and non
-
symbiotic microorganisms, Root nodule

formation
-

Nitrogen fixers


Hydrogenase


Nitrogenase


Nif gene


regulation
-

Biochemistry of Nitrogen fixation,Rhizosphere
-

R: S ratio, Interaction of microbes with
plants. Bioconversion of agricultural wastes.


UNIT


V

Biofertilizer
-

Rhizobium, Az
ospirillum, Azotobacter ,

Gluconacetobacter, Azorhizobium
,
phosphobacteria, Plant Growth Promoting Rhizobacteria ( PGPR)
-

Blue Green Algae
(BGA) and
Azolla

-

Production and quality control of biofertilizers, field application and
crop response. Biopestici
de
-

Bacillus thuringiensis
, insect viruses, entemopathogenic
fungi.

M
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,
,


2
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.


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,


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20


TEXT BOOKS

1.

Joseph C. Deniel, 1996, Environmental aspects of microbiology, British Sun
Publication, Chennai.

2.

Abbasi, S.A. 1998.
Environmental pollution and its control. Cogent Internation
al
publishers, Pondicherry.

3.

Keya Sen and Nicholas J. Ashbolt 2010.
Environmental Microbiology: Current
Technology and Water Applications.

4.

Josdand, S.N., 1995. Environmental Biotechnology. Himalaya Publishing House,
Bombay.

5.

Maier, R.M., Pepper, I,L., Gerba,

C.P. 2009. Environmental Microbiology IInd
Edition
Elsevier Publisher.

6.

Metcalf, R.L. and Luckmann, W.H. 1994. Introduction to insect pest management 3
rd
edn. John Willey and Sons, Inc.

7.

Subba Rao, N.S., 1999. Biofertilizers in Agriculture and Agroforestry.

Oxford and
IBH, New Delhi.

8.

Rangaswami, G. and D.J. Bhagyaraj, 2001. Agricultural Microbiology. 2
nd

Edition.
Prentice Hall, New Delhi.

9.

Rao, N.S., 1995. Soil Microorganisms and plant Growth. Oxford and IBH Publishing
Co., New Delhi.


REFERENCES

1.

Alexan
der, M., 1977. Introduction to soil Microbiology, John Wiley and Sons. New
York

2.

Atlas, R.M. and M. Bartha, 2000. Microbial Ecology
-

Fundamental and Applications.
3
rd
Edition. Redwood City CA. Benjamin/Cumming Science Publishing Co.,New Delhi.

3.

Maier, R.M.,
I.L. Pepper and C.P. Gerba, 2000. Environmental Microbiology.1
st

Edition. Academic Press. New York.

4.

Mitchell, R., 1992. Introduction to Environmental Microbiology; Prentice Hall. Inc.
Englewood Clifs
-

New Jersey.

5.

Motsara, M.R., P. Bhattacharyya and B. Sriv
astava, 1995.
Biofertilizer
-

Technology,

6.

Marketing and Usage. Fertilizer Development and Consultant Organization, New
Delhi.

7.

Pelzar, M.J. and M. Reid,
2003. Microbiology. 5
th

Edition. Tata Mc Graw
-
Hill. New
York.

8.

Reinheimer, G., 1991: Aquatic Microbiolo
gy. 4
th

Edition. John Wiley and Sons. New
York.

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,


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.


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,


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,


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.


21

Semester


II

1
1
MBP211



ADVANCED PRACTICAL


III


6H


3C


SCOPE

This practical is to provide the student with a basic knowledge in microbial biotechnology
and acquire knowledge of the advanced techniques
at molecular levels


OBJECTIVE

To o
btain a good outstanding practical skill in various techniques in microbial
biotechnology and agricultural microbiology


1.

Isolation of plasmid DNA from Bacteria

2.

Isolation of chromosomal DNA from Bacteria

3.

Estimation of m
olecular weight by SDS Polyacrylamide gel
electrophoresis

4.

Restriction mapping of Lambda (

) or vector DNA.

5.

Isolation of microbes from soil

6.

Isolation of free
-
living N
2

fixation

7.

Isolation of symbiotic nitrogen fixers

8.

Isolation of phosphate solublisers

9.

Determination of Dissolved oxygen of water

10.

Determination of BOD (Biochemical Oxygen Demand) of water

11.

Determination of COD (Chemical Oxygen Demand) of water


REFERENCES

1.

Aneja K.R., 2001. Experiments in Microbiology, Plant Pathology, Tissue Culture and

2.

Mush
room Production Technology, 3
rd

Edition, New Age International (P) Limited

Publishers, New Delhi

3.

Cappucino, J.G. and N. Sherman, 2001. Microbiology A Laboratory Manual, 6th
Edition,Benjamin Cummings, New York.

4.

Chirikjan, J.G., E.C. Kisailus, B. King, R.
Krasner and H. Mortensen,
1995.Biotechnology.

5.

Theory and Techniques, Vol II, Jones and Bartlett Publishers, London.

6.

Palanivelu, P., 2004. Analytical Biochemistry and Separation Techniques, 3
rd

Edition,Twenty First Century Publication, Madurai.
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,


2
2
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1
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.


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,


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.


22


Semester
-

II

1
1
MBP212



ADVANCED PRACTICAL


IV


6H


3C


SCOPE

This practical provides provides the current medical aspects on the clinical diagnosis of
infection providing the combined treatment of bacteriology and virology.


OBJECTIVE


To acquire practical kn
owledge in numerous diagnostic tests and procedures used in the
microbiology laboratory.


1.

Laboratory diagnosis of pyogenic infections


tuberculosis


enteric fever


diarrhea




UTI


anaerobic infections

2.

Isolation and identification of clinically

important bacteria
Staphylococcus
sp
., E.

coli,


Salmonella
sp
., Pseudomonas
sp.,

Proteus
sp.,

Klebsiella
sp.

3.

Isolation and identification of
Candida albicans

4.

Antibiotic sensitivity test disc preparation

5.

Antibiotic sensitivity test


Kirby
-

Bauer,

Stroke’s method

6.

MIC determination by Broth dilution technique

7.

MIC determination by filter paper disc assay

8.

Wet mount preparation of parasites
-
Saline, iodine

9.

Identification of parasites
-
formal ether concentration, floatation methods

10.

Morphological examinati
on of fungi in tissues

11.

Germ tube technique

12.

Cultivation of viruses
-
Egg inoculation

13.

Isolation of coli phage from sewage using membrane filter technique


REFERENCES

1.

Arora, B. and D.R. Arora, 2007. Practical Microbiology, 1
st

Edition, CBS Publishers
and Distr
ibutors, Bangalore.

2.

Cappucino, G.J., and N. Sherman, 2001. Microbiology A Laboratory Manual. 6
th

Edition. Benjamin Cummings, New York.

3.

Ellenj O
Baron and Sydneym
Finegold, 1990.
Bailey and

Scott’s Diagnostic Microbiology.
8th Edition,

C V Mosby Company,

St Louis.

4.

Gaud, R.S. and G.D. Gupta, 1999. Practical Microbiology.
1
st

Edition. Nirali
Prakashan, Pune.

5.

Mukherjee, K.L. 2005. Medical Laboratory Technology, Vol. III, Tata McGraw
-
Hill
Publishing Company Ltd, New Delhi.

6.

Reddy, S. M. and S. R. Reddy, 200
4. Microbiology A Laboratory Manual. 3
rd

Edition.
Sri Padmavathi Publication, Hyderabad.

7.

Vandepilte, J., J. Verhaegan, K. Engbaek, P. Rohner, P. Prot and C.C. Heuck, 2004.
Basic Laboratory Procedures in Clinical Bacteriology. 2
nd

Edition, A.I.T.B.S Publis
hers
and Distributors, Delhi.

8.

Sundararaj,T. Microbiology laboratory manual. Aswathy Sundararaj Publishers.
Chennai.

M
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,


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,
,


2
2
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0
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1
.
.


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U
U
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r
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s
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,
,


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6
6
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0
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2
2
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.
.


23

Semester


II

1
1
NMP201



MUSHROOM CULTIVATION



2H


2C


SCOPE

To create awareness on mushroom cultivation technology, spawn production a
nd
utilization.


OBJECTIVES


1. Create better understanding of indigenous mushroom species with a potential for
domestication and
commercialization
.

2. Conserve genetic resources of cultivated mushroom species for public distribution and
research withi
n southern Africa.

3. Demonstrate value addition of cultivated medicinal mushrooms through production of
immunity
-
boosting mushroom nutriceuticals.


UNIT
-

I

Historical Development


Introduction


Types of Mushrooms.


UNIT
-

II

Mushroom fermentation
-

Su
bstrate Fermentation


Carbon to Nitrogen ratio (C / N)


water


Acidity requirement.


UNIT
-

III

Substrate Preparation


Phase


I; Composting
-

Windrow formation


Temperature


Oxygen Requirement


Bed preparation for Spawn production.


UNIT
-

IV

Subs
trate Preparation


Phase
-

II; Composting
-

Elimination of ammonia


Pasteurization
of composting


Spawn production


Spawning.


UNIT
-

V

Induction of fruit bodies
-

Mushroom formation
-

Fruiting body formation


Picking and
packaging
-
Processing problem
s
-

Summary.


REFERENCES

1.

Aneja, K.R., 2001. Experiments in Microbiology Plant Pathology, Tissue culture and
Mushroom Production Technology New Age International (P) Limited. New Delhi.

2.

Frazier, W.C. and D.C. Westhoff, 1995. Food Microbiology. Tata McGraw
-

Hill
Publishing Company Limited. New Delhi.

3.

Jay, J.M., 2000. Modern Food Microbiology. CBS Publishers and Distributors. New
Delhi.


M
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6
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.


24

Semester
-

III

1
1
MBP301



ADVANCED IMMUNOLOGY


4H


4C


SCOPE



Imparting advanced technological knowledge through a det
ailed study of topics such as
immunodiagnosis, assessment of cell mediated immunity and current trends in immunology
of diseases.


OBJECTIVES

To strengthen the knowledge of students through a detailed study on the immune system,
their structure and classif
ication, genetic control of antibody production, Types, structure
of antigens and immunodiagnostics, Molecular immunology, hypersensitive immune
reaction and Latest trends in immunology


UNIT


I

Immunity


types. Cells of the immune system
-

Lymphoid c
ells, Mononuclear cells
,
Granulocytic cells and Mast cells
. T & B


cell maturation
,

a
ctivation and differentiation.
Organs of the immune system
-

Primary & Secondary lymphoid organs


Cutaneous /
Mucosal
-

associated lymphoid tissues


UNIT


II

Antigens
-

Factor influence immunogenicity
-

Epitopes
-

Haptens
-

Study of antigenicity.
Immunoglobulins


structure


Isotypes


Biological activities. Antigenic determinants, Ig
superfamily and Organization & expression of Immunoglobulin genes. Monoclonal
antibod
ies


UNIT


III

Antigen processing and presentation. Transplantation immunology, Transplantation
antigens, HLA typing. Tumor immunology, treatment of tumors. Immune response to
infectious disease. Inbred mouse and Cell lines used in immunologic research


U
NIT


IV

Hypersensitive reactions


Type. Complement System
-

classical, alternative and lectin
pathways, biological consequences. T
-

cell receptor. Cytokines


Structure, functions and
Receptors. Major Histocompatibility complex, Classes, Structure and i
ts functions


UNIT


V

Antigens
-

Antibody reactions: Agglutination and Precipitation. Complement fixation test,
Immunofluorescence, ELISA, RIA, Immuno electron microscopy. Forensic serology,
Immunohaematology


ABO, RH incompatibility


REFERENCES

1.

Coleman
, R.M., M.F. Lombard and R.E. Sicard, 2000. Fundamentals of Immunology
4
th

Edition. WMC Publications. London.

M
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,


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.


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U
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,


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,


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25

2.

Goldsby, R.A., T.J. K. Barbara and A. Osborne, 2006. Kuby Immunology. 6
th

Edition.
W.H. Freeman and Company, New York.

3.

Hyde, R.M., 2000. NMS
-

Im
munology. 4
th

Edition, Lippincott Williams and Wilkins,
Baltimore.

4.

Janeway, Jr. C.A., P.T. M. Walport and M. J. Shlomchick, 2001. Immunobiology
-

The
Immune System in Health and Disease. 5
th

Edition. Churchill Livingstone
-

Garland
Publishing Company, New
York.

5.

Pathaka, S. and U. Palan, 2005. Immunology


Essentials and Fundamentals. 2
nd

Edition. Capital Publishing Company, New Delhi.

6.

Roitt, I.M., J.J Brostaff and D.K. Male, 2002. Immunology. 6
th

Edition. C.V. Mosby
Publishers. St. Louis.

7.

Ananthanarayanan,

R. and J. Panicker, 2004. Text Book of Microbiology
-

Orient
Longman. New Delhi.


M
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2
2
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.


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,


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,


I
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a






6
6
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4
1
1


0
0
2
2
1
1
.
.


26

Semester
-

III

11
MBP302


FOOD AND INDUSTRIAL MICROBIOLOGY

4H


4C


SCOPE

This paper adds information about the role of microorganisms in many food, beverage and
pharma in
dustries both in production and spoilage processes.


OBJECTIVE
S

To encode the importance of the role of microorganisms in food industries both in
beneficial and harmful ways.

To o
btain a good understanding of industrial microbiology
and become qualified a
s microbiologist in food and other industries.


UNIT


I

Food and microorganisms


Important microorganisms in food


Fungi, Bacteria; Intrinsic
and extrinsic parameters of food affecting microbial growth


Sources of contamination of
food. Food sanitation



indicators of food safety


Coliform bacteria.


UNIT


II

Food preservation


principles


Factors affecting preservation


Food preservation using
temperature


low temperature food preservation


characteristics of psychrotrophs


high
temperature foo
d preservation


characteristics of thermophiles


preservation of foods by
drying chemicals and radiation


limitations


commercial application.


UNIT


III

Food borne diseases
-

Food poisoning
-

Food borne infection and Intoxication
-

Food
control agenci
es
-
Microbiological criteria for food, microbial quality control and food laws,
Hazard Analysis Critical Control Point (HACCP).


UNIT


IV

History and chronological development of industrial microbiology. Industrially important
strains


Isolation and prese
rvation.
-

Inoculum development for various fermentation
processes
-

Strain development


mutation, recombinant DNA technology and protoplast
fusion. Fermentation


submerged, solid state, batch and continuous.


UNIT


V

Fermentor design


scale
-
up process
. Types of fermentors
-

Tower, cylindroconical, airlift
and Components of CSTR. Down stream process


intracellular and extracellular product
separation column chromatography, affinity. Production of beverages


beer, vitamins
-

vitamin B12, Riboflavin, an
tibiotics.


TEXT BOOKS

1.

Frazier, W.C. and D.C. Westhoff, 1995. Food Microbiology. Tata McGraw
-
Hill
Publishing Company Limited. New Delhi.

2.

Banwart, G.J., 2004. Basic Food Microbiology. 2
nd

Edition. CBS Publishers and
Distributors New Delhi.

M
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,


2
2
0
0
1
1
1
1
.
.


K
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a
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U
U
n
n
i
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e
r
r
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i
i
t
t
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,
,


C
C
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o
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m
m
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b
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,
,


T
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,
,


I
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d
i
i
a
a






6
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0
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.


27

3.

Patel, A.H., 20
03. Industrial Microbiology, Macmillan India Ltd. New Delhi.

4.

Casida, L.E. Jr., 2003. Industrial Microbiology, New Age International Publishers,
New Delhi.

5.

Shuler, M.L. and F. Kargi, 2005. Bioprocess Engineering Basic Concepts. Pearson
Education, New Del
hi.

6.

Doyle, M.P., R. L. Beuchat and T. J. Montuille, 2001. Food Microbiology


Fundamentals and Frontiers. ASM press.


REFERENCES

1.

Atlas, R.N. and Bartha, 2000. Microbial Ecology
-

Fundamental and Applications.
3rd
Edition. Redwood City CA. Benjamin/Cumming
Science Publishing Co., New Delhi.

2.

Gould, G.W., 1996. New Methods of Food Preservation. Blackie Academic and
Professional, Madras.

3.

Jay, J.M., 2000. Modern Food Microbiology. CBS Publishers and Distributors. New
Delhi.

4.

Mansi,E.M.T.and C.F.A. Bryce, 2002.Fe
rmentation Microbiology and Biotechnology.
Taylor and Francis, New York.

5.

Reed, G., (Editor) 2002. Presscott and Dunn’s Industrial Microbiology. 5
th

Edition.
CBS Publishers, New Delhi.

6.

Stanbury, P.T. and A. Whittaker, 2005. Principles of Fermentation Techno
logy.
Pergamon Press, NY.

7.

Waites, M.J. 2007. Industrial Microbiology, Blackwell Publishing. UK.

M
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,


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2
2
0
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1
1
1
1
.
.


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U
U
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,


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,


I
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a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


28

Semester


III

1
1
MBP303


GENOMICS AND PROTEOMICS


4H


4C


SCOPE

Offers advanced level training on gene sequencing, mapping, expression and
proteomic
techni
ques
.


OBJECTIVES

To emphasize and lay focus on the principle of gene sequencing, mapping, expression,
experimental and analytical proteomics.


UNIT


I

Genome Sequencing: Gene, Genomes, Sequencing Genomes
-

methodology, chain
termination method, chemical

degradation method, shotgun sequencing and assembly of
contiguous DNA sequence. cDNA and genomic library construction.


UNIT


II

Genomic Mapping


Different types of Genome maps and their uses, Genetic and Physical
mapping techniques. Map resources. Pra
ctical uses of genome maps, Genetic Markers
-
RFLP, Mini and Micro satellite, STS and EST, SSCP, RAPD, AFLP, SNPs.


UNIT


III

Gene Expressions and Microarrays

-

Gene structure and pseudo genes. Concepts of
microarrays, spotter analysis, Normalization


to
tal intensity, using regression techniques,
ratio statistics. Clustering Gene expression profiles
-
hierarchical, single
-
linkage, complete
linkage, and average linkage. Tools for microarray analysis
-

MADAM, spot finder, SAGE
Applications of Microarrays
-

Bioi
nformatics challenges in micro array design and analysis.


UNIT


IV

Experimental Proteomics

-

Proteome analysis
-

2
-
D gel electrophoresis: general strategy,
immobilized pH gradients, sample preparation, isoelectric focussing, second dimension
PAGE, staini
ng, transfer of proteins from 2D gels, image acquisition and analysis of 2
-
D
gels.


UNIT


V

Analytical Proteomics:
-
RP
-
HPLC, Mass Spectrometry


ESI MS and MALDI techniques
and applications. Characterization of protein complexes


protein
-
protein interac
tions,
yeast two
-
hybrid system and protein micro arrays.


REFERENCES

1.

Bourneand, P.E. and H. Weissig, 2003. Structural Bioinformatics. John Wiley & Sons
(Asia), Singapore.

2.

Brown, T.A., 2002. Genomes. John Wiley & Sons (Asia) Pvt. Ltd. Singapore.

3.

Cantor, C.R
. and
C.L. Smith
, 1999. Genomics: The Science and Technology behind the
Human Genome Project, John Wiley and Sons (Asia) Pvt. Ltd. Singapor
e.

M
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o
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,


M
M
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o
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b
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g
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,
,


2
2
0
0
1
1
1
1
.
.


K
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a
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a
a
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a
a
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U
U
n
n
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v
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e
e
r
r
s
s
i
i
t
t
y
y
,
,


C
C
o
o
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i
m
m
b
b
a
a
t
t
o
o
r
r
e
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,
,


T
T
N
N
,
,


I
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d
i
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a
a






6
6
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4
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1


0
0
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.


29

4.

Dov Stekal, 2003. Microarray Bioinformatics, Cambridge University Press,

Cambridge,
UK.

5.

Gibson and Muse, 2003. A Primer of Genome Science. Sinauer Associates Inc.

Publishers, Sunderlands, New York.

6.

Liebler, 2001. Introduction to Proteomics, Tools for th
e New Biology. Humana Press,
New Jersey.

7.

Pennington, S. and M.J. Dunn, 2001. Proteomics: From Sequence to Function. Bios
Scientific Pub.Ltd. Oxford, U.K.

8.

Primrose and Twyman, 2003. Principles of Genome Analysis. Blackwell Publishing,
Oxford, UK.

9.

Simpson, R
.P., 2004. Proteins and Proteomics. A Laboratory Manual. Cold

Spring
Harbor Laboratory Press, NY.

10.

Westermeier, R. and T. Naven, 2002. Proteomics in Practice. Wiley


VCH, Weinheim.

M
M
a
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r
r


o
o
f
f


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c
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,
,


M
M
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c
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o
o
b
b
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o
o
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o
o
g
g
y
y
,
,


2
2
0
0
1
1
1
1
.
.


K
K
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a
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p
p
a
a
g
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a
a
m
m


U
U
n
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e
r
r
s
s
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i
t
t
y
y
,
,


C
C
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o
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m
m
b
b
a
a
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o
o
r
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,
,


T
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N
,
,


I
I
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d
i
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6
6
4
4
1
1


0
0
2
2
1
1
.
.


30



Semester
-

III

11
MBP304



BIOSTATISTICS AND RESEARCH METHODOLOGY











4H


4C


Scope

: This course has been intended to provide the student insights into helpful
areas of Statistics , which plays an essential role in present , future use and
applications of Biology.


Objective:
Students get an idea about collection, i
nterpretation and presentation of
statistical data.


UNIT
-
I

Definitions
-
Scope of

Biostatistics
-

Variables in biology, collection, classification and
tabulation of data
-

Graphical and diagrammatic representation.

Measures of central tendency


Arithmetic
mean, median and mode. Measures of
dispersion
-
Range, standard deviation, Coefficient of variation.


UNIT


II

Correlation


Meaning and definition
-

Scatter diagram

Karl pearson’s correlation
coefficient. Rank correlation.

Regression: Regression in two v
ariables


Regression coefficient problems


uses of
regression.


UNIT


III

Test of significance: Tests based on Means only
-
Both Large sample and Small sample tests
-

Chi square test
-

goodness of fit. Analysis of variance


one way and two way
classifica
tion. CRD, RBD Designs.

UNIT


IV

Research: Scope and significance


Types of Research


Research Process


Characteristics of good research


Problems in Research


Identifying research problems.
Research Designs


Features of good designs.

UNIT


V

Sampl
ing Design: Meaning


Concepts


Steps in sampling


Criteria for good sample
design. Scaling measurements


Techniques


Types of scale.


TEXT BOOK:

Jerrold H.Zar, 2003, Biostatistical Analysis, Fourth Edition, Pearson Education (Pte ) .Ltd,
New Delhi.

K
othari. C.R., 2004.
Research Methodology



Methods and Techniques, Second edition,
New Age International Pvt. Ltd, New Delhi.

REFERENCES:

Gupta.S.P., 2001.
Statistical Methods
, Sultan Chand & Co, New Delhi.

Sundar Rao. P.S.S., and J.Richard., 2006.
Introdu
ction to Biostatistics and Research
Methods
, PHI Publication, New Delhi.

Sandhu. T., 1990.
Research Techniques in Biological Sciences
, Anmol Publishers, New
Delhi.

M
M
a
a
s
s
t
t
e
e
r
r


o
o
f
f


S
S
c
c
i
i
e
e
n
n
c
c
e
e
,
,


M
M
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i
c
c
r
r
o
o
b
b
i
i
o
o
l
l
o
o
g
g
y
y
,
,


2
2
0
0
1
1
1
1
.
.


K
K
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a
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r
p
p
a
a
g
g
a
a
m
m


U
U
n
n
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i
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v
e
e
r
r
s
s
i
i
t
t
y
y
,
,


C
C
o
o
i
i
m
m
b
b
a
a
t
t
o
o
r
r
e
e
,
,


T
T
N
N
,
,


I
I
n
n
d
d
i
i
a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


31

Semester


III

1
1
MBP305




CELL BIOLOGY




3C


SCOPE

To understand the basic concep
t of cell structure, membrane, cellular functions of different
types of cell, modes of cellular signaling and signal amplification.


OBJECTIVES

To study cell structure and functions of organelle

Exposure on transportations through cell membrane

To focus

on different receptors and model of signaling

To introduce the concept of cell signaling


UNIT


I



Cell as a basic unit of living systems, t
he cell t
heory, ultrastructure of eukaryotic cell (both
plant and animal). Cell wall structure


bacteria, algae, fungi and plants and their functions.
Extra cellular materials.


UNIT


II





Plasma membrane: structure, origin and functions. Basement membrane, tight junctions,
gap junctions and desmosomes. Cell adhesion and Intercellular recognition. Cytoskeleton
elements: Structure and functions of microtubules, microfilaments and intermediate

filaments.


UNIT


III

Structure and functions of cell organelles


Endoplasmic reticulum, Golgi complex,
Mitochondria, Chloroplast, Ribosomes, Ly
sosomes, Peroxisomes, Nucleus and Vacuoles.


UNIT


IV





Discovery, morphology and structural organization
-

centromere, secondary constriction,
telomere, chromonema, euchromatin and heterochromatin, chemical composition and
karyotype.


UNIT


V





Cell cycle: Mitosis: General events of interphase, five phases of mitosis, physiology and
significance. Meiosis: Phases of meiosis and significance
-

Cellular aging: Morphological,
physiological and sub
-
cellular changes during aging.


REFER
ENCES

1.

Albert, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J.D. Watson, 1989. Molecular
Biology of the Cell, Garland Publishing Inc, London.

2.

Sadava, D.E., 1993. Cell biology: Organelle structure and functions. 1
st

Edition, Jones
and Bartlett Publishers.
USA.

3.

Karp, G., 1984. Cell biology, 2
nd

Edition, Mc Graw
-
Hill Publications. USA.

4.

Gupta, M.L. and M.L. Jangir, 2001. Cell Biology: Fundamentals and Applications, 1
st

Edition, Agrobios, Jodhpur, India.

M
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n
n
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c
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,
,


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c
r
r
o
o
b
b
i
i
o
o
l
l
o
o
g
g
y
y
,
,


2
2
0
0
1
1
1
1
.
.


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K
a
a
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p
a
a
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a
a
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U
U
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e
r
r
s
s
i
i
t
t
y
y
,
,


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b
a
a
t
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o
o
r
r
e
e
,
,


T
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N
,
,


I
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d
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i
a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


32

5.

Verma, P.S. and V.K. Agarwal, 2005. Cell Biology, 24
th

Ed
ition, S. Chand and
Company Limited. India.




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6
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33

Semester
-

III

1
1
MBP311


APPLICATION ORIENTED PRACTICAL


V


6H


3C


SCOPE

This practical is to provide the student with a basic knowledge of immunology and its
significance to human disease.


OBJE
CTIVE
S

The general objectives of the lab will be to introduce immunology and basic serological
techniques.


1.

ABO Blood grouping
-

RH typing and cross matching.

2.

Agglutination tests.



WIDAL
-

slide and tube test



RA test.



RPR test.



ASO test.



CRP test.




-
HCG te
st

3.

ELISA

4.

Ouchterlony’s Double Immunodiffusion test (ODD)

5.

Counter immunoelectrophoresis (CIE)

6.

Antigen Preparation.

7.

Preparation of antisera


REFERENCES

1.

Ellenj O
Baron and Sydneym Finegold, 1990.
Bailey and Scott’s Diagnostic
Microbiology. 8
th

Edition,

C V
Mosby Company, St Louis.

2.

Benson, H.J., 1998. Microbiological Application
-

Laboratory Manual in General
Microbiology. 7
th

Edition. WCB McGraw


Hill, New York.

3.

Talwar, G.P. and S.K. Gupta, 1993. A Handbook of Practical and Clinical
Immunology, 2
nd

Editio
n. Vol. 2, CBS Publishers and Distributors, New Delhi.

4.

Thomas J. Kindt
,
Barbara Anne Osborne
,
Richard A. Goldsby
. 2007. Immunology.
W.H.Freeman.

5.

Coleman, R.M.,Lombard, M.F. and Sic
ard, R.E. 1992. Fundamental Immunology, 2
nd

ed, Dubuque, Iowa:Wm. C.Brown.

6.

Darla J. Wise
,
Gordon R. Carter
.
2002. Immunology: a comprehensive review. Wiley
-
Blackwell.

7.

Janeway, C.A., and Travers, P. 1997, Immunobiology: The immune system in health
and disease, 3d ed. New York, Garland Publishing.

8.

Kuby, J. 1997, Immunology, 3d ed. New York, W.H. Freeman.

9.

Male, D., Champion, B., Cooke, A. and Owen, M. 1991. Advanced immunology.
Mosby publication, Baltimore.

10.

Roitt, I., Brustoff, J. and Male, D. 1999. Immunology, 5
th

Edn. Harcourt Brace and Co.
Asia P
TE Ltd.

M
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,
,


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c
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o
o
b
b
i
i
o
o
l
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o
o
g
g
y
y
,
,


2
2
0
0
1
1
1
1
.
.


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U
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y
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,
,


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C
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,
,


T
T
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N
,
,


I
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d
i
i
a
a






6
6
4
4
1
1


0
0
2
2
1
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.
.


34

Semester
-

III

1
1
MBP312



APPLICATION ORIENTED PRACTICAL


VI 6H


3C


SCOPE

This practical adds a good understanding of industrial microbiology and become qualified
as microbiologist in food and beverage industries.


OBJECTIVE
S

This prov
ides information on fermented food product production in food industries.
T
o
know the possible contamination of food products which may include bacteria and fungi.


1.

Production of enzymes


Solid state & Submerged fermentation


GUS assay


Amylase

2.

Pro
duction of protease from submerged fermentation

3.

Production of cellulase from solid state fermentation

4.

Production of sauerkraut

5.

Production of yoghurt

6.

Production of wine

7.

Enumeration of Microorganisms from Food samples by spread or pour plate method

8.

Detecti
on and enumeration of Microorganisms present in Utensils.

9.

Analysis of Milk quality by MBRT

10.

Detection of coliforms from water
-

MPN test

11.

Isolation of plant pathogens


Bacteria and fungi

12.

Citric acid production

13.

Mushroom Cultivation


REFERENCES

1.

Arora, B. and
D.R. Arora, 2007. Practical Microbiology, 1
st

Edition, CBS Publishers
and Distributors, Bangalore.

2.

Arora, B. and D.R. Arora, 2007. Practical Microbiology, 1
st

Edition, CBS Publishers
and Distributors, Bangalore.

3.

Cappucino, G.J. and N. Sherman, 2001. Micr
obiology A Laboratory Manual. 6
th

Edition. Benjamin Cummings, New York.

4.

Harry, W., Seeley,Jr. and Paul Van Denmark, 1984. Microbes in Actions: A lab Manual
of Microbiology. D. B. Taraporwalla and Sons.

5.

Demain, A.L. and Davies, J.E. 1999. Manual of Industr
ial Microbiology and
Biotechnology IInd Edition. ASM Press, Washington.

6.

Julian E Davies

and
Arnol
d L Demain

2009 Manual of Industrial Microbiology and
Biotechnology ASM Publisher

7.

Richard H Baltz
,
Julian E Davies

and
Arnold L Demain

2010.
Manual of Industrial
Microbiology and Biotechnology 3e ASM Publisher

8.

Ahmed E.Y. and Carlstrom C.

2003.
Food Microbiology: A Labor
atory Manual,
John
Wiley and Sons, Inc. New Jeresy.

M
M
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o
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f


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n
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,
,


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o
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l
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o
g
g
y
y
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,


2
2
0
0
1
1
1
1
.
.


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U
U
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,


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C
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,
,


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,
,


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a






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0
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.
.


35

9.

James M. Jay, Martin J. Loessner, David A. Golden
2005
.
Modern Food Microbiology.
Springer Science, U.S.A.



10.

Garg, N., Garg, K.L. and Mukerji, K.G. 2010. Laboratory Manual of Food
Microbiology. I.K. Inter
national Publishing House. New Delhi.

11.

Adams M. R. and Moss M. O.
2000
Food Microbiology.

Royal Society of Chemistry.
Cambridge, U.K.

M
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1
1
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1
.
.


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U
U
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,


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,
,


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a






6
6
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4
1
1


0
0
2
2
1
1
.
.


36

Semester
-

III

11
NMP301


LABORATORY ANIMAL CARE



2H


2C


SCOPE

Aimed to provide training on various methods of handlin
g, concerning the care and use of
laboratory animal.


OBJECTIVES

Laboratory animal care provides the proper handling and care for various species of
animals used in research, testing, and in education. It extensively deals with the amended
act on the Anima
l Welfare and the concept, availability, and use of research or testing
methods that limit the use of animals or minimize animal distress.


UNIT


I

Modern methods of care, management breeding and maintenance of Laboratory animals


rabbit.


UNIT


II


Modern methods of care, management breeding and maintenance of Laboratory animals


mice.


UNIT


III


Modern methods of care, management breeding and maintenance of Laboratory animals


rat.


UNIT


IV


Modern methods of care, management breeding and m
aintenance of Laboratory animals


guinea pig. Specific pathogen free animal


gnotobiotic animal


UNIT


V

Handling


various routes of inoculation and bleeding. Laboratory use of animals in
microbiology
-

antibody production. Disposal of animal house was
tes and carcasses
.


REFERENCES

1.

Nalinasundari, M.S. and R. Santhi, 2006. Entomology. MJP Publishers, Chennai.

2.

Pelczar, Jr. M.J., E.C.S. Chan and N.R. Kreig, 1993. Microbiology McGraw
-
Hill Inc.
New York.

3.

Prescott, M., J.P. Harley and D.A. Klein, 1993. Microb
iology, 2
nd

Edition. McGraw
-
Hill Inc. New York.

4.

Roy, D.N. and A.W.A. Brown, 2003. Entomology


Medical and Veterinary. 1
st

Edition. Part


I, Biotech Books, New Delhi.

5.

Warren, D. M., 2002. Small Animal Care and Management. 2
nd

Edition. Delmar


Thomson L
earning, Columbia, NY.

6.

Yadav, M., 2004. Applied Entomology. 1
st

Edition. Discovery Publishing house, New
Delhi.

M
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,


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o
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g
y
y
,
,


2
2
0
0
1
1
1
1
.
.


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m


U
U
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y
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,
,


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r
r
e
e
,
,


T
T
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N
,
,


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n
d
d
i
i
a
a






6
6
4
4
1
1


0
0
2
2
1
1
.
.


37

Semester


III


1
1
MBP491



PROJECT AND VIVA VOCE




12C