12-13 DNA and genetic engineering.ppt - SBP-Portal

deadstructureBiotechnology

Dec 14, 2012 (4 years and 10 months ago)

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DNA

RNA

Amino
Acids

Creating
Variation

Manipulate
DNA

Bio
-

technology


100

Science Jeopardy!


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Question: What are the four nitrogenous bases?

DNA 100


Answer: Adenine, Thymine, Guanine, and
Cytosine.

Question: What is the principle of base pairing?


Answer: When Adenine and Thymine pair
and Guanine and Cytosine pair.


DNA 200


Answer: Deoxyribose, a phosphate
group, and a base (A/T/C/G).

Question: What is a nucleotide?

DNA 300

DNA 4
00

Question: What is the complementary
strand for the following DNA
-

ATTCTAGCC?


Answer: The complimentary DNA
strand reads
-

TAAGATCGG


Answer: Griffith observed sick mice after
what combination of bacteria?

Question:
What is Griffith mixed heat
-
killed
bacteria with live harmless bacteria?


DNA 500


Answer: This base is unique to RNA.


Question: What is Uracil?

RNA 100




Answer:
Sugar is ribose, composed of single
-
stands,
and contain Uricil
-

U instead of Thymine
-
T


RNA 200

Question: What are the structure differences of RNA compared
to DNA?


Answer:
mRNA (instructions to assemble aa to
proteins); rRNA (proteins assembled); tRNA
(construction of protein, coding/ transfer)


RNA 300

Question: What are the 3 types of RNA?




Answer: The final mRNA cuts out introns but
the ______ are spliced back together.

RNA 400

Question: What are
exons
?



Question: What is a frame shift
mutation.


Answer:

Adding a nucleotide to codons changes the
amino acids from this point on.


RNA 500




Answer: The order of 3 nitrogenous bases.

Question: What is a codon.

Amino Acids
100


Answer: On page 303, this is the sequence for
Methionine.

Answer: What is AUG?

Amino Acids
200



Answer: On page 303 this is the sequence for
tryptophan.

Question: What is UGG?

Amino Acids
300

Amino Acids
400


Answer: On Page 303 these are the
sequences to make Glutatmic Acid.

Question:

What is GAG and GAA?



Answer: This is the job of the stop codon.

Question: What is signify the end of
a polypeptide (many amino acids)?

Amino Acids
500



Creating Variation
100


Answer: The naturally occurring way to
create variation in a population.

Question: What is a mutation?

Creating Variation
200


Answer: The reason eukaryotic gene
regulation is more complex.

Questions: What is cell specialization?

Creating Variation
300


Answer: A group of genes that
work together and are
expressed.

Question: What is the operon?

Creating Variation
400



Answer: These are turned off by
repressors and on by lactose.

Question: What are lac genes?

Creating Variation
500


Answer: To position RNA polymerase and
have a marking point just before
transcription begins.

Question: What is the job of the TATA
box in eukaryotes?

Manipulate DNA
100



Answer: Using naturally occurring
genetic events to select desired
traits for next generation


Question: What is selective breeding?

Manipulate DNA
200


Answer: When crossing dissimilar
organisms to bring together the best
of both


Question: What is hybridization?

Manipulate DNA
300


Answer: The continued breeding of
individuals with similar desired
characteristics but has risks.


Question: What is inbreeding?

Manipulate DNA
400


Answer: A technique where can make many
copies of a particular gene.


Question: What is
PCR (Polymerase Chain Rxn) ?

Question: What is
Gel Electrophoresis ?

Manipulate DNA
500


Answer: Separate DNA and put DNA
in gel, electric voltage is added, and
-

charged move towards + end of
gel.



Answer: This scientist created over 800 varieties
of plants.

Bio
-
technology
100

Question: Who is Burbank?


Answer: When a cell takes in DNA from an
outside cell, and that DNA becomes part of
cell’s DNA.

Bio
-
technology
200

Question: What is Transformation?

Answer:


1 has DNA sequence to promote plasmid
replication (replicates new DNA)


2 has Genetic Marker
-
gene that
distinguishes between bacteria with
plasmid and those without


Bio
-
technology
300


Question: What are reasons we
use plasmids?

Bio
-
technology
400

Answer: Using the following organisms,
containing genes from other species:


Microorganisms: reproduce rapidly/ grow
quickly


Animals: study genes/ improve food supply


Plants: main food supply/ eliminated
pesticides


Question: What is
Transgenic organisms?

Bio
-
technology
500


Answer: A nucleus of an egg fused with cell
from adult was then placed in foster mom
sheep to develop normally.

Question: What is the cloning of
Dolly?