Using bioinformatics for better understanding of genes amplify

dasypygalstockingsBiotechnology

Oct 2, 2013 (3 years and 8 months ago)

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Using bioinformatics for
better understanding of
genes

Becky
Seipelt

Karla Marriott

Arlin

Toro

Vilma

Martinez

Roger
Lui


http://artedi.ebc.uu.se/course/BioInfo
-
10p
-
2006/projects/katarzyna/bioinformatics.jpg


DNA


RNA


PROTEIN


Function


phenotype

Promoters

Gene finding


Variation

Probability


DOGMA


Transcription

Splicing

Editing

Modifications


Human Gene
Variation

Translation

Editing

Modifications

Structure

Complexes


TRANSLATE

Structure

Complexes

1. Gene finding

2. Case study using a normal and
a known mutant gene

Ideas

A FEW Resources

Proposed activities

Becky Seipelt, Middle
Tennessee State University

rseipelt@mtsu.edu



Active learning activity


Perhaps animation comparison


Give
a small sequence file with 2
-
5
genes within.


Ask
students to figure out how
the
cell “knows
” where a gene is
, i.e.,
where to start
and stop
transcription
, and where to
start
and stop translation.


Introduce ‘gene finding’
algorithms for comparison
(
DOGMA
)


Ask students to compare how
“we” and nature differ?


Honors Genetics (BIOL3250H)


2 blocks of 3 hours


Sophomore
-
seniors, mostly
sophomores


Transcription and translation
“learned” at least twice, perhaps
more


Goals:


deeper understanding of
processes
and

regulation


more engaged students


Lab exercise
-
Mitochondrial DNA Analysis


http://www.edvotek.com/images/332.jpg

Students will use
DOGMA to identify
genes that they
amplify with PCR



Working with the Sequence

Blast and other analysis

evolutionary problems

How this project using DOGMA will help me teaching
my genetics course


The

next

time

I

teach

the

part

of

genomes

and

proteomes

in

my

genetics

course,

in

the

explanation

of

c o m p a r a t i v e

g e n o m i c s,

I

c a n

s h o w

s i m i l a r i t i e s

b e t w e e n

d i f f e r e n t

g e n o m e s

a n d

i n t r o d u c e

t h e m

t h e

e v o l u t i o n a r y

r e l a t i o n s h i p s

b e t w e e n

o r g a n i s m s

of

different

species

and

the

information

that

some

s p e c i e s

a r e

m o r e

r e l a t e d

t h a n

o t h e r s
.


Computational and systems biology

7
-
week course.

For comp. bio. part, either do sequence
alignment problem if more math majors OR


bioinformatics using data and software
available on the web like what I have
learned in this conference.



Development of new course targeting seniors and
juniors


Involves lecture and laboratory


Research
-

DNA sequencing, translation and
transcription to protein


Using software such as DOGMA, Translate, BLAST